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![Knockout Validated: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot. The total protein (20 ug) of the dentate gyrus, extracted from the wild-type (lanes 1 and 3) and Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, and transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta-Tubulin is a positive control for the Western blot. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-B01P-img0006.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot. The total protein (20 ug) of the dentate gyrus, extracted from the wild-type (lanes 1 and 3) and Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, and transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta-Tubulin is a positive control for the Western blot. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: TDO2 Antibody [H00006999-B01P] - Analysis of TDO2 expression in transfected 293T cell line by TDO2 polyclonal antibody. Lane 1: TDO2 transfected lysate(44.66 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-B01P-img0005.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, and the cerebellum. In the cerebellum, the immunoreactivity for TDO was observed in granule cells and Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDO2-Antibody-Immunocytochemistry-Immunofluorescence-H00006999-B01P-img0009.jpg)
![Western Blot: TDO2 Antibody [H00006999-B01P] - Analysis of TDO2 expression in human liver.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-B01P-img0004.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, and the cerebellum. A TDO was not stained in the SVZ. The immunoreactivities for TDO in wild type and Tdo-/- mice were background level. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDO2-Antibody-Immunocytochemistry-Immunofluorescence-H00006999-B01P-img0007.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, and the cerebellum. TDO was not stained in the olfactory bulb (OB). The immunoreactivities for TDO in wild type and Tdo-/- mice were background level. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDO2-Antibody-Immunocytochemistry-Immunofluorescence-H00006999-B01P-img0008.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by immunofluorescent staining. The hippocampal sections of wild-type and Tdo-/- mice were stained with anti-TDO antibody. In sections of Tdo -/- mice, immunofluorescence signals were not detected in granule cells, interneurons (arrowhead), CA1, or CA3 cells, suggesting that the secondary antibody used in this study bound specifically to the primary antibody (mouse IgG). g, granule cell layer; h, hilus. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TDO2-Antibody-Immunohistochemistry-H00006999-B01P-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, & the cerebellum. A & B: TDO was not stained in the SVZ (A) & the olfactory bulb (OB) (B). The immunoreactivities for TDO in wild type & Tdo-/- mice were background level. C: In the cerebellum, the immunoreactivity for TDO was observed in granule cells & Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. E, external plexiform layer; G, granule cell layer; Gm, glomerular layer; I, internal plexiform layer; LV, lateral ventricle; Mi, mitral cell layer; Mo, molecular layer; P, Purkinje cell layer; St, striatum; Su, subependymal zone. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415384127.jpg)
![Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, & the cerebellum. A & B: TDO was not stained in the SVZ (A) & the olfactory bulb (OB) (B). The immunoreactivities for TDO in wild type & Tdo-/- mice were background level. C: In the cerebellum, the immunoreactivity for TDO was observed in granule cells & Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. E, external plexiform layer; G, granule cell layer; Gm, glomerular layer; I, internal plexiform layer; LV, lateral ventricle; Mi, mitral cell layer; Mo, molecular layer; P, Purkinje cell layer; St, striatum; Su, subependymal zone. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415171317.jpg)
![Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, & the cerebellum. A & B: TDO was not stained in the SVZ (A) & the olfactory bulb (OB) (B). The immunoreactivities for TDO in wild type & Tdo-/- mice were background level. C: In the cerebellum, the immunoreactivity for TDO was observed in granule cells & Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. E, external plexiform layer; G, granule cell layer; Gm, glomerular layer; I, internal plexiform layer; LV, lateral ventricle; Mi, mitral cell layer; Mo, molecular layer; P, Purkinje cell layer; St, striatum; Su, subependymal zone. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415394527.jpg)
![Western Blot: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot (A) & immunofluorescent staining (B). A: The total protein (20 μg) of the dentate gyrus, extracted from the wild-type (lanes 1 & 3) & Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, & transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta -Tubulin is a positive control for the Western blot. B: The hippocampal sections of wild-type & Tdo-/- mice were stained with anti-TDO antibody. In sections of Tdo-/- mice, immunofluorescence signals were not detected in granule cells, interneurons (arrowhead), CA1, or CA3 cells, suggesting that the secondary antibody used in this study bound specifically to the primary antibody (mouse IgG). g, granule cell layer; h, hilus. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415483615.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot (A) & immunofluorescent staining (B). A: The total protein (20 μg) of the dentate gyrus, extracted from the wild-type (lanes 1 & 3) & Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, & transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta -Tubulin is a positive control for the Western blot. B: The hippocampal sections of wild-type & Tdo-/- mice were stained with anti-TDO antibody. In sections of Tdo-/- mice, immunofluorescence signals were not detected in granule cells, interneurons (arrowhead), CA1, or CA3 cells, suggesting that the secondary antibody used in this study bound specifically to the primary antibody (mouse IgG). g, granule cell layer; h, hilus. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415485113.jpg)
![Western Blot: TDO2 Antibody (OTI2A4) [NBP2-45995] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDO2.](http://images.novusbio.com/fullsize/TDO2-Antibody-2A4-Western-Blot-NBP2-45995-img0010.jpg "Western Blot: TDO2 Antibody (OTI2A4) [NBP2-45995] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDO2.")
![Immunohistochemistry-Paraffin: TDO2 Antibody (OTI2A4) [NBP2-45995] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-TDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/TDO2-Antibody-2A4-Immunohistochemistry-NBP2-45995-img0009.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (OTI2A4) [NBP2-45995] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/TDO2-Antibody-2A4-Immunohistochemistry-NBP2-45995-img0001.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (OTI2A4) [NBP2-45995] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.,](http://images.novusbio.com/fullsize/TDO2-Antibody-2A4-Immunohistochemistry-NBP2-45995-img0002.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (OTI2A4) [NBP2-45995] - Staining of paraffin-embedded Human breast tissue within the normal limits using anti-TDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/TDO2-Antibody-2A4-Immunohistochemistry-NBP2-45995-img0003.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (OTI2A4) [NBP2-45995] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-TDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/TDO2-Antibody-2A4-Immunohistochemistry-NBP2-45995-img0004.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (OTI2A4) [NBP2-45995] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-TDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/TDO2-Antibody-2A4-Immunohistochemistry-NBP2-45995-img0005.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (OTI2A4) [NBP2-45995] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/TDO2-Antibody-2A4-Immunohistochemistry-NBP2-45995-img0006.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (OTI2A4) [NBP2-45995] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/TDO2-Antibody-2A4-Immunohistochemistry-NBP2-45995-img0007.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (OTI2A4) [NBP2-45995] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TDO2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/TDO2-Antibody-2A4-Immunohistochemistry-NBP2-45995-img0008.jpg)
![Western Blot: TDO2 Antibody (1D1) [NBP2-02002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDO2.](http://images.novusbio.com/fullsize/TDO2-Antibody-1D1-Western-Blot-NBP2-02002-img0001.jpg "Western Blot: TDO2 Antibody (1D1) [NBP2-02002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDO2.")
![Western Blot: TDO2 Antibody [H00006999-D01P] - Analysis of TDO2 expression in Jurkat.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-D01P-img0003.jpg "Western Blot: TDO2 Antibody [H00006999-D01P] - Analysis of TDO2 expression in Jurkat.")
![Western Blot: TDO2 Antibody [H00006999-D01P] - Analysis of TDO2 expression in transfected 293T cell line by TDO2 polyclonal antibody.Lane 1: TDO2 transfected lysate(47.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-D01P-img0004.jpg)
![Western Blot: TDO2 Antibody (OTI1D1) - Azide and BSA Free [NBP2-74485] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDO2.](http://images.novusbio.com/fullsize/TDO2-Antibody-OTI1D1-Azide-and-BSA-Free-Western-Blot-NBP2-74485-img0001.jpg "Western Blot: TDO2 Antibody (OTI1D1) - Azide and BSA Free [NBP2-74485] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDO2.")
![Western Blot: TDO2 Overexpression Lysate (Adult Normal) [NBL1-16799] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TDO2.](http://images.novusbio.com/fullsize/TDO2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16799-img0002.jpg "Western Blot: TDO2 Overexpression Lysate (Adult Normal) [NBL1-16799] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TDO2.")
![SDS-Page: Recombinant Human TDO2 Protein [NBP1-51014] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-TDO2-Protein-SDS-Page-NBP1-51014-img0001.jpg "SDS-Page: Recombinant Human TDO2 Protein [NBP1-51014] - 15 % SDS-PAGE (3ug)")