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![Western Blot: SYK Antibody [NBP1-32945] - SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody at a dilution of 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0012.jpg "Western Blot: SYK Antibody [NBP1-32945] - SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody at a dilution of 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32945-img0002.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded rat spleen dilution: 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0010.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - Sample (30 ug of whole cell lysate) A: BMDM (bone marrow-derived macrophage) cell B: Syk null cell 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0004.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0005.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - Mouse tissue extracts (50 ug) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with SYK diluted by 1:1000.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0007.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody at a dilution of 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NBP1-32945-img0011.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded MDA-MB468 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0003.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human cervical cancer diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0008.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human endometrium diluted at 1:500.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-32945-img0009.jpg)
![Immunoprecipitation: SYK Antibody [NBP1-32945] - Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 ug of SYK antibody. Western blot analysis was performed using SYK antibody.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunoprecipitation-NBP1-32945-img0013.jpg)
![Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Western-Blot-NBP3-09107-img0001.jpg "Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control")
![Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunocytochemistry-Immunofluorescence-NBP3-09107-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunocytochemistry-Immunofluorescence-NBP3-09107-img0002.jpg)
![Knockout Validated: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Knockout-Validated-NBP1-03250-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Western blot of extracts from Human PBMC, SYK Antibody (SYK-01)](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Western-Blot-NBP1-03250-img0002.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody (SYK-01) [NBP1-03250] - Staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunohistochemistry-Paraffin-NBP1-03250-img0001.jpg)
![Western Blot: SYK Antibody (2G11E6) [NBP2-61893] - Analysis using SYK mAb against human SYK (AA: 217-356) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Western-Blot-NBP2-61893-img0004.jpg "Western Blot: SYK Antibody (2G11E6) [NBP2-61893] - Analysis using SYK mAb against human SYK (AA: 217-356) recombinant protein. (Expected MW is 41.4 kDa)")
![Immunocytochemistry/Immunofluorescence: SYK Antibody (2G11E6) [NBP2-61893] - Analysis of Hela cells using SYK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Immunocytochemistry-Immunofluorescence-NBP2-61893-img0003.jpg)
![Flow Cytometry: SYK Antibody (2G11E6) [NBP2-61893] - Analysis of Hela cells using SYK mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Flow-Cytometry-NBP2-61893-img0002.jpg)
![Western Blot: SYK Antibody (2G11E6) [NBP2-61893] - Analysis using SYK mAb against HEK293 (1) and SYK (AA: 217-356)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Western-Blot-NBP2-61893-img0005.jpg)
![ELISA: SYK Antibody (2G11E6) [NBP2-61893] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-ELISA-NBP2-61893-img0001.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-86178] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-86178-img0007.jpg "Immunohistochemistry-Paraffin: SYK Antibody [NBP1-86178] - Staining of human tonsil shows high expression.")
![Immunohistochemistry-Paraffin: SYK Antibody [NBP1-86178] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-86178-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SYK Antibody [NBP1-86178] - Staining in human tonsil and liver tissues using anti-SYK antibody. Corresponding SYK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SYK-Antibody-Immunohistochemistry-Paraffin-NBP1-86178-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: SYK [p Tyr526, p Tyr525] Antibody (PSH03-70) [NBP3-32800] - Immunocytochemistry analysis of Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes labeling SYK with Rabbit anti-SYK antibody (NBP3-32800) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SYK antibody (NBP3-32800) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32800_rabbit-syk-p-tyr526-p-tyr525-mab-psh03-70-29202410234235.jpg "Immunocytochemistry/ Immunofluorescence: SYK [p Tyr526, p Tyr525] Antibody (PSH03-70) [NBP3-32800] - Immunocytochemistry analysis of Ramos cells serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes labeling SYK with Rabbit anti-SYK antibody (NBP3-32800) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SYK antibody (NBP3-32800) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: SYK [p Tyr526, p Tyr525] Antibody (PSH03-70) [NBP3-32800] - Western blot analysis of SYK on different lysates with Rabbit anti-SYK antibody (NBP3-32800) at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Ramos serum starved for 16 hours add 12μg/mL human IgM (diluted in serum free medium) for 2 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-2 (left): 1 minute 2 seconds; Lane 1-2 (right): 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32800_rabbit-syk-p-tyr526-p-tyr525-mab-psh03-70-3920248453519.jpg)
![Western Blot: SYK Antibody [NB100-1796] - Analysis of SYK in Human Spleen lysate using this antibody at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SYK-Antibody-Western-Blot-NB100-1796-img0003.jpg "Western Blot: SYK Antibody [NB100-1796] - Analysis of SYK in Human Spleen lysate using this antibody at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: SYK Antibody (M373) [NBP3-23427] - Western blot of adult mouse spleen lysate. The blot was probed with mouse monoclonal anti-SYK (Central region) antibody at 1:250.](http://images.novusbio.com/fullsize/nbp3-23427_mouse-syk-mab-m373-199202312523615.jpg "Western Blot: SYK Antibody (M373) [NBP3-23427] - Western blot of adult mouse spleen lysate. The blot was probed with mouse monoclonal anti-SYK (Central region) antibody at 1:250.")
![Western Blot: SYK Overexpression Lysate (Adult Normal) [NBL1-16637] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SYK.](http://images.novusbio.com/fullsize/SYK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16637-img0002.jpg "Western Blot: SYK Overexpression Lysate (Adult Normal) [NBL1-16637] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SYK.")