Hu,
Mu,
Bv,
Ca,
Eq,
Pm,
Rt,
Po,
Pm,
Pm-Or,
RMSpecies Glossary
Reactivity: Hu, Mu, Bv, Ca, Eq, Pm, Rt, Po, Pm, Pm-Or, RM
Applications: WB, IHC
Host: Rabbit Polyclonal
Alt Catalog# IMG-6059A
Sample Size Available
Sizes: Sample Size Available
![Western Blot: STRAP Antibody [NBP2-24740] - Analysis of human STRAP in Raw cell lysate in the 1) absence and 2) presence of immunizing peptide using this NBP2-24740 at 1 ug/ml.](http://images.novusbio.com/fullsize/STRAP-Antibody-Western-Blot-NBP2-24740-img0002.jpg "Western Blot: STRAP Antibody [NBP2-24740] - Analysis of human STRAP in Raw cell lysate in the 1) absence and 2) presence of immunizing peptide using this NBP2-24740 at 1 ug/ml.")
![Immunohistochemistry-Paraffin: STRAP Antibody [NBP2-24740] - IHC analysis of human spleen using NBP2-24740 at 10 ug/ml.](http://images.novusbio.com/fullsize/STRAP-Antibody-Immunohistochemistry-Paraffin-NBP2-24740-img0001.jpg)
![Western Blot: STRAP Antibody [NBP2-20521] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/STRAP-Antibody-Western-Blot-NBP2-20521-img0008.jpg "Western Blot: STRAP Antibody [NBP2-20521] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: STRAP Antibody [NBP2-20521] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: STRAP protein stained by STRAP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/STRAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20521-img0010.jpg)
![Immunohistochemistry-Paraffin: STRAP Antibody [NBP2-20521] - Paraffin-embedded mouse stomach. STRAP antibody [N1C3] dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/STRAP-Antibody-Immunohistochemistry-Paraffin-NBP2-20521-img0009.jpg)
![Western Blot: STRAP Antibody [NBP2-20521] - A. 30 ug MCF-7 whole cell lysate/extract B. 30 ug MDA-MB-231 whole cell lysate/extract 10% SDS-PAGE STRAP antibody [N1C3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/STRAP-Antibody-Western-Blot-NBP2-20521-img0002.jpg)
![Western Blot: STRAP Antibody [NBP2-20521] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/STRAP-Antibody-Western-Blot-NBP2-20521-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: STRAP Antibody [NBP2-20521] - Wild-type (WT) and STRAP knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/STRAP-Antibody-Western-Blot-NBP2-20521-img0011.jpg)
![Western Blot: STRAP Antibody [NBP2-20521] - STRAP antibody [N1C3] detects STRAP protein by western blot analysis.A. 30 ug MCF-7 whole cell lysate/extractB. 30 ug MDA-MB-231 whole cell lysate/extract 10% SDS-PAGESTRAP antibody [N1C3] (NBP2-20521) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20521_rabbit-polyclonal-strap-antibody-910202420134017.jpg)
![Western Blot: STRAP Antibody [NBP1-82796] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4.](http://images.novusbio.com/fullsize/STRAP-Antibody-Western-Blot-NBP1-82796-img0005.jpg "Western Blot: STRAP Antibody [NBP1-82796] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4.")
![Immunohistochemistry-Paraffin: STRAP Antibody [NBP1-82796] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/STRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-82796-img0009.jpg)
![Western Blot: STRAP Antibody [NBP1-82796] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/STRAP-Antibody-Western-Blot-NBP1-82796-img0006.jpg)
![Immunohistochemistry-Paraffin: STRAP Antibody [NBP1-82796] - Staining of human cerebellum shows moderate cytoplasmic and strong nucleolar positivity in Purkinje cells.](http://images.novusbio.com/fullsize/STRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-82796-img0007.jpg)
![Immunohistochemistry-Paraffin: STRAP Antibody [NBP1-82796] - Staining of human kidney shows moderate cytoplasmic, moderate membranous and strong nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/STRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-82796-img0008.jpg)
![Immunohistochemistry-Paraffin: STRAP Antibody [NBP1-82796] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/STRAP-Antibody-Immunohistochemistry-Paraffin-NBP1-82796-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: STRAP Antibody [NBP3-37924] - Immunofluorescence analysis of L929 cells using STRAP Rabbit pAb at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37924_rabbit-strap-pab-912202415133494.jpg "Immunocytochemistry/ Immunofluorescence: STRAP Antibody [NBP3-37924] - Immunofluorescence analysis of L929 cells using STRAP Rabbit pAb at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: STRAP Antibody [NBP3-37924] - Immunofluorescence analysis of H9C2 cells using STRAP Rabbit pAb at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37924_rabbit-strap-pab-912202414163067.jpg)
![Immunocytochemistry/ Immunofluorescence: STRAP Antibody [NBP3-37924] - Immunofluorescence analysis of U2OS cells using STRAP Rabbit pAb at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37924_rabbit-strap-pab-912202414154325.jpg)
![Immunoprecipitation: STRAP Antibody [NBP3-37924] - Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug STRAP antibody. Western blot was performed from the immunoprecipitate using STRAP antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-37924_rabbit-strap-pab-912202414112878.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: STRAP Antibody [NBP3-37924] - Western Blot analysis of lysates from wild type(WT) and STRAP knockout (KO) HeLa cells, using STRAP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-37924_rabbit-strap-pab-31120251704761.jpg%3Ft=1702606360)
![Western Blot: STRAP Antibody [NBP2-38757] - Analysis using Anti-STRAP antibody NBP2-38757 (A) shows similar pattern to independent antibody NBP2-56502 (B).](http://images.novusbio.com/fullsize/STRAP-Antibody-Western-Blot-NBP2-38757-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: STRAP Antibody [NBP2-38757] - Analysis using Anti-STRAP antibody NBP2-38757 (A) shows similar pattern to independent antibody NBP2-56502 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: STRAP Antibody [NBP2-38757] - Staining of human cell line CACO-2 shows positivity in cytoplasm and nucleus but excluded from the nucleoli.](http://images.novusbio.com/fullsize/STRAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38757-img0001.jpg)
![Immunohistochemistry-Paraffin: STRAP Antibody [NBP2-38757] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/STRAP-Antibody-Immunohistochemistry-NBP2-38757-img0002.jpg)
![Western Blot: STRAP Antibody [NBP2-56502] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/STRAP-Antibody-Western-Blot-NBP2-56502-img0002.jpg "Western Blot: STRAP Antibody [NBP2-56502] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: STRAP Antibody [NBP2-56502] - Staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/STRAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56502-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: STRAP Antibody [NBP2-56502] - Analysis using Anti-STRAP antibody NBP2-56502 (A) shows similar pattern to independent antibody NBP2-38757 (B).](http://images.novusbio.com/fullsize/STRAP-Antibody-Western-Blot-NBP2-56502-img0003.jpg)
![Immunoprecipitation:STRAP AntibodyNBP3-30067] -Detection of human STRAP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-STRAP antibody NBP3-30067 (lot NBP3-30067-1) used for IP at 6 µg per reaction. STRAP was also immunoprecipitated by rabbit anti-STRAP antibody BL18472. For blotting immunoprecipitated STRAP, NBP3-30067 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30067_rabbit-strap-pab-301220241515496.jpg "Immunoprecipitation:STRAP AntibodyNBP3-30067] -Detection of human STRAP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-STRAP antibody NBP3-30067 (lot NBP3-30067-1) used for IP at 6 µg per reaction. STRAP was also immunoprecipitated by rabbit anti-STRAP antibody BL18472. For blotting immunoprecipitated STRAP, NBP3-30067 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
