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![Western Blot: SMG1 Antibody (1A8) [H00023049-M02] - SMG1 monoclonal antibody (M02), clone 1A8 Analysis of SMG1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/SMG1-Antibody-1A8-Western-Blot-H00023049-M02-img0007.jpg "Western Blot: SMG1 Antibody (1A8) [H00023049-M02] - SMG1 monoclonal antibody (M02), clone 1A8 Analysis of SMG1 expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: SMG1 Antibody (1A8) [H00023049-M02] - Analysis of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SMG1-Antibody-1A8-Immunohistochemistry-Paraffin-H00023049-M02-img0005.jpg)
![Sandwich ELISA: SMG1 Antibody (1A8) [H00023049-M02] - Detection limit for recombinant GST tagged SMG1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMG1-Antibody-1A8-Sandwich-ELISA-H00023049-M02-img0006.jpg)
![Western Blot: SMG1 Antibody [NB100-2320] - Detection of Human SMG1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMG1 antibody NB100-2320 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SMG1-Antibody-Western-Blot-NB100-2320-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMG1 Antibody [NB100-2320] - Detection of Human SMG1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMG1 antibody NB100-2320 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: SMG1 Antibody [NB100-2320] - Detection of Human SMG1 on HeLa whole cell lysate using NB100-2320. IP of SMG1 was successful when using affinity purified rabbit anti-SMG1 antibodies NB100-2321 and another SMG1 antibody.](http://images.novusbio.com/fullsize/SMG1-Antibody-Western-Blot-NB100-2320-img0001.jpg)
![Western Blot: SMG1 Antibody (1C12) [H00023049-M03] - Analysis of SMG1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/SMG1-Antibody-1C12-Western-Blot-H00023049-M03-img0005.jpg "Western Blot: SMG1 Antibody (1C12) [H00023049-M03] - Analysis of SMG1 expression in Hela S3 NE (Cat # L013V3).")
![Immunohistochemistry-Paraffin: SMG1 Antibody (1C12) [H00023049-M03] - Analysis of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/SMG1-Antibody-1C12-Immunohistochemistry-Paraffin-H00023049-M03-img0004.jpg)
![ELISA: SMG1 Antibody (1C12) [H00023049-M03] - Detection limit for recombinant GST tagged SMG1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMG1-Antibody-1C12-ELISA-H00023049-M03-img0003.jpg)
![Western Blot: SMG1 Antibody [NB100-2321] - Detection of human SMG1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMG1 antibody NB100-2321 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/SMG1-Antibody-Western-Blot-NB100-2321-img0004.jpg "Western Blot: SMG1 Antibody [NB100-2321] - Detection of human SMG1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMG1 antibody NB100-2321 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunoprecipitation: SMG1 Antibody [NB100-2321] - Detection of human SMG1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMG1 antibody NB100-2321 (lot 2) used for IP at 6 ug per reaction. SMG1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated SMG1, NB100-2321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SMG1-Antibody-Immunoprecipitation-NB100-2321-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SMG1 Antibody (1G5) [H00023049-M01] - Analysis of monoclonal antibody to SMG1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SMG1-Antibody-1G5-Immunocytochemistry-Immunofluorescence-H00023049-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: SMG1 Antibody (1G5) [H00023049-M01] - Analysis of monoclonal antibody to SMG1 on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: SMG1 Antibody (1G5) [H00023049-M01] - Detection limit for recombinant GST tagged SMG1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMG1-Antibody-1G5-Sandwich-ELISA-H00023049-M01-img0005.jpg)
![Western Blot: SMG1 Antibody [NB100-77278] - HeLa whole cell lysate using NB100-77278. SMG1 was also immunoprecipitated by rabbit anti-SMG1 antibodies NB100-2320 and NB100-2321.](http://images.novusbio.com/fullsize/SMG1-Antibody-Western-Blot-NB100-77278-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SMG1 Antibody [NB100-77278] - HeLa whole cell lysate using NB100-77278. SMG1 was also immunoprecipitated by rabbit anti-SMG1 antibodies NB100-2320 and NB100-2321.")
![Western Blot: SMG1 Antibody (5N10C7) [NBP3-15716] - Western blot analysis of extracts of various cell lines, using SMG1 antibody (NBP3-15716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SMG1-Antibody-5N10C7-Western-Blot-NBP3-15716-img0001.jpg "Western Blot: SMG1 Antibody (5N10C7) [NBP3-15716] - Western blot analysis of extracts of various cell lines, using SMG1 antibody (NBP3-15716) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: SMG1 Antibody (SR1684) [NBP3-22354] - Western blot analysis using NBP3-22354 in Saos2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22354_rabbit-smg1-mab-sr1684-672023106246.jpg "Western Blot: SMG1 Antibody (SR1684) [NBP3-22354] - Western blot analysis using NBP3-22354 in Saos2 cell lysate.")
![SDS-Page: Recombinant Human SMG1 Protein [H00023049-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00023049-Q01-1.jpg "SDS-Page: Recombinant Human SMG1 Protein [H00023049-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: SMG1 Antibody (2B10) [H00023049-M04] - Analysis of SMG1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/SMG1-Antibody-2B10-Western-Blot-H00023049-M04-img0003.jpg "Western Blot: SMG1 Antibody (2B10) [H00023049-M04] - Analysis of SMG1 expression in Hela S3 NE (Cat # L013V3).")
![ELISA: SMG1 Antibody (2B10) [H00023049-M04] - Detection limit for recombinant GST tagged SMG1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMG1-Antibody-2B10-ELISA-H00023049-M04-img0002.jpg)
![Western Blot: SMG1 Antibody (4E4) [H00023049-M05] - Analysis of SMG1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/SMG1-Antibody-4E4-Western-Blot-H00023049-M05-img0004.jpg "Western Blot: SMG1 Antibody (4E4) [H00023049-M05] - Analysis of SMG1 expression in Hela S3 NE (Cat # L013V3).")
![Sandwich ELISA: SMG1 Antibody (4E4) [H00023049-M05] - Detection limit for recombinant GST tagged SMG1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SMG1-Antibody-4E4-Sandwich-ELISA-H00023049-M05-img0003.jpg)
![Immunohistochemistry-Paraffin: SMG1 Antibody [NBP1-88913] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SMG1-Antibody-Immunohistochemistry-Paraffin-NBP1-88913-img0004.jpg "Immunohistochemistry-Paraffin: SMG1 Antibody [NBP1-88913] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.")