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![Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RFC1-Antibody-Western-Blot-NB100-230-img0089.jpg "Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFC1 antibody (lot was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: RFC1 Antibody [NB100-230] - Samples: Whole cell lysate (50, 15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RFC1-Antibody-Western-Blot-NB100-230-img0088.jpg)
![Immunocytochemistry/Immunofluorescence: RFC1 Antibody [NBP2-54960] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RFC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54960-img0001.jpg "Immunocytochemistry/Immunofluorescence: RFC1 Antibody [NBP2-54960] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
![Western Blot: RFC1 Antibody [NB600-961] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RFC1 antibody NB600-961 (lot 2 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. RFC1 was also immunoprecipitated by prior lot 1 of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/RFC1-Antibody-Western-Blot-NB600-961-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: RFC1 Antibody [NB600-961] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RFC1 antibody NB600-961 (lot 2 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. RFC1 was also immunoprecipitated by prior lot 1 of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Immunocytochemistry/Immunofluorescence: RFC1 Antibody [NBP2-56364] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/RFC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56364-img0001.jpg "Immunocytochemistry/Immunofluorescence: RFC1 Antibody [NBP2-56364] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")