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![Immunohistochemistry-Paraffin: PRPF6 Antibody [NBP1-92298] - Staining in human fallopian tube and pancreas tissues using anti-PRPF6 antibody. Corresponding PRPF6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PRPF6-Antibody-Immunohistochemistry-Paraffin-NBP1-92298-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PRPF6 Antibody [NBP1-92298] - Staining in human fallopian tube and pancreas tissues using anti-PRPF6 antibody. Corresponding PRPF6 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PRPF6 Antibody [NBP1-92298] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/PRPF6-Antibody-Western-Blot-NBP1-92298-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PRPF6 Antibody [NBP1-92298] - Staining of human cell line CACO-2 shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PRPF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92298-img0005.jpg)
![Immunohistochemistry-Paraffin: PRPF6 Antibody [NBP1-92298] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/PRPF6-Antibody-Immunohistochemistry-Paraffin-NBP1-92298-img0006.jpg)
![Immunohistochemistry-Paraffin: PRPF6 Antibody [NBP1-92298] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PRPF6-Antibody-Immunohistochemistry-Paraffin-NBP1-92298-img0007.jpg)
![Western Blot: PRPF6 Antibody [NBP1-46212] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PRP6 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody NBP1-46213, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/PRPF6-Antibody-Western-Blot-NBP1-46212-img0005.jpg "Western Blot: PRPF6 Antibody [NBP1-46212] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PRP6 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody NBP1-46213, which recognizes a downstream epitope.")
![Immunohistochemistry: PRPF6 Antibody [NBP1-46212] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PRPF6-Antibody-Immunohistochemistry-NBP1-46212-img0003.jpg)
![Western Blot: PRPF6 Antibody [NBP1-46213] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PRP6 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody NBP1-46212, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/PRPF6-Antibody-Western-Blot-NBP1-46213-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PRPF6 Antibody [NBP1-46213] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-PRP6 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody NBP1-46212, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: PRPF6 Antibody [NBP1-46213] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PRPF6-Antibody-Immunohistochemistry-NBP1-46213-img0002.jpg)
![Western Blot: PRPF6 Antibody [NBP2-19965] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PRPF6-Antibody-Western-Blot-NBP2-19965-img0001.jpg "Western Blot: PRPF6 Antibody [NBP2-19965] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: PRPF6 Antibody [NBP1-57544] - Transfected 293T cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/PRPF6-Antibody-Western-Blot-NBP1-57544-img0003.jpg "Western Blot: PRPF6 Antibody [NBP1-57544] - Transfected 293T cell lysate, concentration 2.5 ug/ml.")
![Western Blot: PRPF6 Overexpression Lysate (Adult Normal) [NBL1-14824] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRPF6.](http://images.novusbio.com/fullsize/PRPF6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14824-img0002.jpg "Western Blot: PRPF6 Overexpression Lysate (Adult Normal) [NBL1-14824] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRPF6.")