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![Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase A regulatory subunit I alpha monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-6C7-Western-Blot-NBP1-47935-img0009.jpg "Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase A regulatory subunit I alpha monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase A regulatory subunit I alpha.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-6C7-Immunocytochemistry-Immunofluorescence-NBP1-47935-img0010.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (OTI6C7) [NBP1-47935] - Staining of paraffin-embedded carcinoma of bladder tissue (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, Dilution 1:50).](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-OTI6C7-Immunohistochemistry-Paraffin-NBP1-47935-img0012.jpg)
![Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase A regulatory subunit I alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protein Kinase A regulatory subunit I alpha.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-6C7-Western-Blot-NBP1-47935-img0001.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-6C7-Immunohistochemistry-Paraffin-NBP1-47935-img0002.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded colon tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-6C7-Immunohistochemistry-Paraffin-NBP1-47935-img0004.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded endometrium tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-6C7-Immunohistochemistry-Paraffin-NBP1-47935-img0005.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded Ovary tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-6C7-Immunohistochemistry-Paraffin-NBP1-47935-img0006.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody (6C7) [NBP1-47935] - Staining of paraffin-embedded prostate tissue using anti-Protein Kinase A regulatory subunit I alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-6C7-Immunohistochemistry-Paraffin-NBP1-47935-img0007.jpg)
![Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-33585] - Lane 1: Marker [kDa] 250,130,100,70,55,35,25,15,10. Lane 2: Human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Western-Blot-NBP2-33585-img0005.jpg "Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-33585] - Lane 1: Marker [kDa] 250,130,100,70,55,35,25,15,10. Lane 2: Human cell line SK-MEL-30.")
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-33585] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-33585-img0010.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-33585] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-33585-img0001.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-33585] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-33585-img0007.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-33585] - Staining of human pancreas shows low positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-33585-img0008.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-33585] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-33585-img0009.jpg)
![Simple Western: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-33585] - Lane view shows a specific band for Protein Kinase A regulatory subunit I alpha using 200 ug/mL of Sk-Mel-28 cell lysate and 4 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 54 kDa. Image from an internal validation.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Simple-Western-NBP2-33585-img0011.jpg)
![Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-06561] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:43 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Western-Blot-NBP3-06561-img0001.jpg "Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-06561] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:43 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-06561] - Staining of PRKAR1A in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human PRKAR1A polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06561-img0003.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-06561] - Staining of human PRKAR1A in human brain with rabbit polyclonal antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Immunohistochemistry-Paraffin-NBP3-06561-img0002.jpg)
![Immunoprecipitation: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-06561] - Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-PRKAR1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PRKAR1A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size :45 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Immunoprecipitation-NBP3-06561-img0004.jpg)
![Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289] - Anti-Protein Kinase A regulatory subunit I alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 49 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Western-Blot-NBP3-00289-img0005.jpg "Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289] - Anti-Protein Kinase A regulatory subunit I alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 49 kDa (We are unsure as to the identity of these extra bands).")
![<b>Genetic Strategies Validation. </b>Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289] - Anti-Protein Kinase A regulatory subunit I alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: Protein Kinase A regulatory subunit I alpha knockout Hela Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 49 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Western-Blot-NBP3-00289-img0006.jpg)
![Immunoprecipitation: Protein Kinase A regulatory subunit I alpha Antibody [NBP3-00289] - Protein Kinase A regulatory subunit I alpha was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. Lane C: 0.5 mg 293T Whole Cell Lysate. 2 ul anti-Protein Kinase A regulatory subunit I alpha rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Protein Kinase A regulatory subunit I alpha rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Immunoprecipitation-NBP3-00289-img0004.jpg)
![Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-93193] - Western blot analysis of extracts from normal (control) and Protein Kinase A regulatory subunit I alpha knockout (KO) 293T cells, using Protein Kinase A regulatory subunit I alpha antibody (NBP2-93193) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Western-Blot-NBP2-93193-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-93193] - Western blot analysis of extracts from normal (control) and Protein Kinase A regulatory subunit I alpha knockout (KO) 293T cells, using Protein Kinase A regulatory subunit I alpha antibody (NBP2-93193) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-93193] - Analysis of NIH/3T3 cells using Protein Kinase A regulatory subunit I alpha at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93193-img0001.jpg)
![Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [NBP2-93193] - Analysis of extracts of various cell lines, using Protein Kinase A regulatory subunit I alpha at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-Western-Blot-NBP2-93193-img0003.jpg)
![Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (1C3) [NBP3-15192] - Positive WB detected in: U87 whole cell lysate. All lanes: Protein Kinase A regulatory subunit I alpha antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 39 KDa. Observed band size: 43 KDa.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-1C3-Western-Blot-NBP3-15192-img0002.jpg "Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (1C3) [NBP3-15192] - Positive WB detected in: U87 whole cell lysate. All lanes: Protein Kinase A regulatory subunit I alpha antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 39 KDa. Observed band size: 43 KDa.")
![Immunocytochemistry/Immunofluorescence: Protein Kinase A regulatory subunit I alpha Antibody (1C3) [NBP3-15192] - Immunofluorescence staining of Hela cells with at Protein Kinase A regulatory subunit I alpha antibody 1:36, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-1C3-Immunocytochemistry-Immunofluorescence-NBP3-15192-img0001.jpg)
![SDS-Page: Recombinant Human Protein Kinase A regulatory subunit I alpha Protein [H00005573-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005573-P01-1.jpg "SDS-Page: Recombinant Human Protein Kinase A regulatory subunit I alpha Protein [H00005573-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5) [NBP3-15086] - Western blot detection of Protein Kinase A regulatory subunit I alpha/PRKAR1A in C6, Rat Brain, Jurkat cell lysates using Protein Kinase A regulatory subunit I alpha/PRKAR1A Rabbit mAb (1:500 diluted). Predicted band size: 43kDa. Observed band size: 43kDa](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Antibody-S06-8A5-Western-Blot-NBP3-15086-img0001.jpg "Western Blot: Protein Kinase A regulatory subunit I alpha Antibody (S06-8A5) [NBP3-15086] - Western blot detection of Protein Kinase A regulatory subunit I alpha/PRKAR1A in C6, Rat Brain, Jurkat cell lysates using Protein Kinase A regulatory subunit I alpha/PRKAR1A Rabbit mAb (1:500 diluted). Predicted band size: 43kDa. Observed band size: 43kDa")
![SDS-Page: Recombinant Human Protein Kinase A regulatory subunit I alpha Protein [H00005573-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005573-Q01-1.jpg "SDS-Page: Recombinant Human Protein Kinase A regulatory subunit I alpha Protein [H00005573-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Protein Kinase A regulatory subunit I alpha Overexpression Lysate (Adult Normal) [NBP2-04485] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein Kinase A regulatory subunit I alpha.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04485-img0001.jpg "Western Blot: Protein Kinase A regulatory subunit I alpha Overexpression Lysate (Adult Normal) [NBP2-04485] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein Kinase A regulatory subunit I alpha.")
![Western Blot: Protein Kinase A regulatory subunit I alpha Overexpression Lysate (Adult Normal) [NBP2-04233] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein Kinase A regulatory subunit I alpha.](http://images.novusbio.com/fullsize/Protein-Kinase-A-regulatory-subunit-I-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04233-img0001.jpg "Western Blot: Protein Kinase A regulatory subunit I alpha Overexpression Lysate (Adult Normal) [NBP2-04233] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein Kinase A regulatory subunit I alpha.")