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![Western Blot: Cyclophilin 40 Antibody (PSH02-71) [NBP3-32238] - Western blot analysis of Cyclophilin 40 on different lysates with Rabbit anti-Cyclophilin 40 antibody (NBP3-32238) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Jurkat cell lysate Lane 7: COS-1 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 41 kDa Observed band size: 47 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32238) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32238_rabbit-cyclophilin-40-mab-psh02-71-278202415212476.jpg "Western Blot: Cyclophilin 40 Antibody (PSH02-71) [NBP3-32238] - Western blot analysis of Cyclophilin 40 on different lysates with Rabbit anti-Cyclophilin 40 antibody (NBP3-32238) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: K-562 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: Jurkat cell lysate Lane 7: COS-1 cell lysate Lane 8: Human liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 41 kDa Observed band size: 47 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32238) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cyclophilin 40 Antibody (PSH02-71) [NBP3-32238] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cyclophilin 40 antibody (NBP3-32238) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32238) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32238_rabbit-cyclophilin-40-mab-psh02-71-29820241492647.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyclophilin 40 Antibody (PSH02-71) [NBP3-32238] - Immunocytochemistry analysis of 293T cells labeling Cyclophilin 40 with Rabbit anti-Cyclophilin 40 antibody (NBP3-32238) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cyclophilin 40 antibody (NBP3-32238) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32238_rabbit-cyclophilin-40-mab-psh02-71-2920241114830.jpg)
![Flow Cytometry: Cyclophilin 40 Antibody (PSH02-71) [NBP3-32238] - Flow cytometric analysis of 293T cells labeling Cyclophilin 40. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32238, 1:100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32238_rabbit-cyclophilin-40-mab-psh02-71-29202416372171.jpg)
![Western Blot: Cyclophilin 40 Antibody [NBP1-85364] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-Western-Blot-NBP1-85364-img0004.jpg "Western Blot: Cyclophilin 40 Antibody [NBP1-85364] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: Cyclophilin 40 Antibody [NBP1-85364] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & cytosol.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85364-img0006.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin 40 Antibody [NBP1-85364] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-Immunohistochemistry-Paraffin-NBP1-85364-img0002.jpg)
![Western Blot: Cyclophilin 40 Antibody (4C7) [H00005481-M01] - Analysis of PPID expression in transfected 293T cell line by PPID monoclonal antibody (M01), clone 4C7.Lane 1: PPID transfected lysate (Predicted MW: 40.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-4C7-Western-Blot-H00005481-M01-img0004.jpg "Western Blot: Cyclophilin 40 Antibody (4C7) [H00005481-M01] - Analysis of PPID expression in transfected 293T cell line by PPID monoclonal antibody (M01), clone 4C7.Lane 1: PPID transfected lysate (Predicted MW: 40.8 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Cyclophilin 40 Antibody (4C7) [H00005481-M01] - Analysis of monoclonal antibody to PPID on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-4C7-Immunocytochemistry-Immunofluorescence-H00005481-M01-img0002.jpg)
![Sandwich ELISA: Cyclophilin 40 Antibody (4C7) [H00005481-M01] - Detection limit for recombinant GST tagged PPID is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-4C7-Sandwich-ELISA-H00005481-M01-img0003.jpg)
![Western Blot: Cyclophilin 40 Antibody [NBP1-85363] - Analysis in control (vector only transfected HEK293T lysate) and PPID over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-Western-Blot-NBP1-85363-img0008.jpg "Western Blot: Cyclophilin 40 Antibody [NBP1-85363] - Analysis in control (vector only transfected HEK293T lysate) and PPID over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Cyclophilin 40 Antibody [NBP1-85363] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-Immunohistochemistry-Paraffin-NBP1-85363-img0007.jpg)
![Western Blot: Cyclophilin 40 Antibody [NBP1-85363] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-Western-Blot-NBP1-85363-img0006.jpg)
![Western Blot: Cyclophilin 40 Antibody (4O7P5) [NBP3-16552] - Western blot analysis of extracts of various cell lines, using Cyclophilin 40 Rabbit mAb (NBP3-16552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-4O7P5-Western-Blot-NBP3-16552-img0002.jpg "Western Blot: Cyclophilin 40 Antibody (4O7P5) [NBP3-16552] - Western blot analysis of extracts of various cell lines, using Cyclophilin 40 Rabbit mAb (NBP3-16552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Cyclophilin 40 Antibody (4O7P5) [NBP3-16552] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Cyclophilin 40 Rabbit mAb (NBP3-16552) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-4O7P5-Immunohistochemistry-Paraffin-NBP3-16552-img0001.jpg)
![Western Blot: Cyclophilin 40 Antibody (SR1688) [NBP3-22135] - Western blot analysis using NBP3-22135 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22135_rabbit-cyclophilin-40-mab-sr1688-5720231523496.jpg "Western Blot: Cyclophilin 40 Antibody (SR1688) [NBP3-22135] - Western blot analysis using NBP3-22135 in K562 cell lysate.")
![Western Blot: Cyclophilin 40 Antibody [NB120-3567] - Analysis of rat heart extract.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-Western-Blot-NB120-3567-img0008.jpg "Western Blot: Cyclophilin 40 Antibody [NB120-3567] - Analysis of rat heart extract.")
![Western Blot: Cyclophilin 40 Antibody [NB120-3567] - Analysis of rat brain extract.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-Western-Blot-NB120-3567-img0009.jpg)
![Western Blot: Cyclophilin 40 Antibody (1B8) [NBP2-60239] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPID antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: K562 cell lysateLane 2.: Jurkat cell lysate Lane 3.: HeLa cell lysate Lane 4.: HepG2 cell lysateLane 5.: A549 cell lysateLane 6.: MCF7 cell lysateLane 7.: SK-OV-3 cell lysateLane 8.: PC3 cell lysate](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-1B8-Western-Blot-NBP2-60239-img0001.jpg "Western Blot: Cyclophilin 40 Antibody (1B8) [NBP2-60239] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPID antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: K562 cell lysateLane 2.: Jurkat cell lysate Lane 3.: HeLa cell lysate Lane 4.: HepG2 cell lysateLane 5.: A549 cell lysateLane 6.: MCF7 cell lysateLane 7.: SK-OV-3 cell lysateLane 8.: PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: Cyclophilin 40 Antibody (1B8) [NBP2-60239] - Staining in Hep3B cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-1B8-Immunocytochemistry-Immunofluorescence-NBP2-60239-img0002.jpg)
![Western Blot: Cyclophilin 40 Antibody [NB100-61665] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cyclophilin-40-Antibody-Western-Blot-NB100-61665-img0001.jpg "Western Blot: Cyclophilin 40 Antibody [NB100-61665] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Western Blot: Cyclophilin 40 Overexpression Lysate (Adult Normal) [NBL1-14648] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cyclophilin 40.](http://images.novusbio.com/fullsize/Cyclophilin-40-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14648-img0002.jpg "Western Blot: Cyclophilin 40 Overexpression Lysate (Adult Normal) [NBL1-14648] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cyclophilin 40.")
![Western Blot: Cyclophilin 40 Overexpression Lysate (Adult Normal) [NBL1-14648] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for PPID](http://images.novusbio.com/fullsize/Cyclophilin-40-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14648-img0001.jpg)
![SDS-Page: Recombinant Human Cyclophilin 40 His Protein [NBC1-18427] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human Cyclophilin 40 His Protein-SDS-Page-NBC1-18427-img0003.jpg "SDS-Page: Recombinant Human Cyclophilin 40 His Protein [NBC1-18427] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![SDS-Page: Recombinant Mouse Cyclophilin 40 Protein [NBP2-61451] - 15% SDS-Page (3ug)](http://images.novusbio.com/fullsize/Recombinant-Mouse-Cyclophilin-40-Protein-SDS-Page-NBP2-61451-img0001.jpg "SDS-Page: Recombinant Mouse Cyclophilin 40 Protein [NBP2-61451] - 15% SDS-Page (3ug)")