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![Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Type: 293T. Antibody Dilution: 1.0ug/mlPITX3 is supported by BioGPS gene expression data to be expressed in HEK293T](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85481-img0002.jpg "Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Type: 293T. Antibody Dilution: 1.0ug/mlPITX3 is supported by BioGPS gene expression data to be expressed in HEK293T")
![Western Blot: PITX3 Antibody [NBP2-85481] - WB Suggested Anti-PITX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysate](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85481-img0001.jpg)
![Western Blot: PITX3 Antibody [NBP2-85481] - Host: Rabbit. Target Name: PITX3. Sample Tissue: Human Hela Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85481-img0003.jpg)
![Western Blot: PITX3 Antibody [NB100-96910] - (1ug/ml) staining of Human Cer(35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NB100-96910-img0003.jpg "Western Blot: PITX3 Antibody [NB100-96910] - (1ug/ml) staining of Human Cer(35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PITX3 Antibody [NBP2-85479] - Host: Rabbit. Target Name: PITX3. Sample Type: Human Fetal Liver. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85479-img0002.jpg "Western Blot: PITX3 Antibody [NBP2-85479] - Host: Rabbit. Target Name: PITX3. Sample Type: Human Fetal Liver. Antibody Dilution: 1.0ug/ml")
![Western Blot: PITX3 Antibody [NBP2-85479] - WB Suggested Anti-PITX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85479-img0001.jpg)
![Western Blot: PITX3 Antibody [NBP2-85479] - Host: Rabbit. Target Name: PITX3. Sample Tissue: Human RPMI 8226 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85479-img0003.jpg)
![Western Blot: PITX3 Antibody (SR1536) [NBP3-22365] - Western blot analysis using NBP3-22365 in U87-MG cell lysate.](http://images.novusbio.com/fullsize/nbp3-22365_rabbit-pitx3-mab-sr1536-5720231456545.jpg "Western Blot: PITX3 Antibody (SR1536) [NBP3-22365] - Western blot analysis using NBP3-22365 in U87-MG cell lysate.")
![Western Blot: PITX3 Antibody [NBP2-94198] - Analysis of extracts of various cell lines, using PITX3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-94198-img0001.jpg "Western Blot: PITX3 Antibody [NBP2-94198] - Analysis of extracts of various cell lines, using PITX3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Western Blot: PITX3 Antibody (3P4U8) [NBP3-15674] - Western blot analysis of extracts of various cell lines, using PITX3 antibody (NBP3-15674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PITX3-Antibody-3P4U8-Western-Blot-NBP3-15674-img0001.jpg "Western Blot: PITX3 Antibody (3P4U8) [NBP3-15674] - Western blot analysis of extracts of various cell lines, using PITX3 antibody (NBP3-15674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: PITX3 Antibody (28A9) [NBP3-26380] - Staining of A549 with PITX3 Antibody (28A9) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 520-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26380_rabbit-pitx3-mab-28a9-28220241312236.jpg "Immunocytochemistry/Immunofluorescence: PITX3 Antibody (28A9) [NBP3-26380] - Staining of A549 with PITX3 Antibody (28A9) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 520-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: PITX3 Antibody (28A9) [NBP3-26380] - Overlay Peak curve showing SH-SY5Y cells stained with PITX3 Antibody (28A9) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26380_rabbit-pitx3-mab-28a9-282202414183631.jpg)
![Western Blot: PITX3 Antibody [NBP2-85482] - WB Suggested Anti-PITX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85482-img0001.jpg "Western Blot: PITX3 Antibody [NBP2-85482] - WB Suggested Anti-PITX3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate")
![Western Blot: PITX3 Antibody [NBP2-85483] - Host: Rabbit. Target Name: PITX3. Sample Type: NCI-H226 Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/PITX3-Antibody-Western-Blot-NBP2-85483-img0001.jpg "Western Blot: PITX3 Antibody [NBP2-85483] - Host: Rabbit. Target Name: PITX3. Sample Type: NCI-H226 Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: PITX3 Overexpression Lysate (Adult Normal) [NBL1-14449] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PITX3.](http://images.novusbio.com/fullsize/PITX3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14449-img0002.jpg "Western Blot: PITX3 Overexpression Lysate (Adult Normal) [NBL1-14449] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PITX3.")