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![Ablation of AAV-PdynP+ spinal dorsal horn (SDH) neurons does not affect behavioral responses evoked by the optical stimulation of A beta fibers, nociceptive mechanical force, and heat in rats. (A) Enhanced green fluorescent protein (EGFP) expression (green) in the L4 SDH at 4 weeks after intra-SDH microinjection of AAV-PdynP-DTR-EGFP (indicated by an upper schematic illustration). Colocalization of EGFP and PAX2 (red) in the SDH (indicated by arrowheads). Scale bar, 100 μm. The percentage of co-expressing neurons was quantified (n = 3 rats tested, 94 total EGFP+ cells quantified, and 339 total PAX2+ cells quantified). (B) Diphtheria toxin receptors (DTR) immunofluorescence in the SDH after administration of diphtheria toxin (DTX: 50 μg/kg, i.p., two injection 72 h apart) or PBS. Scale bar, 200 μm. (C) Immunohistochemical analysis and quantification of PAX2+ neurons in the superficial laminae of the ipsilateral and contralateral SDH at 4 weeks after DTX injection (n = 6 rats tested, PAX2+ neurons: 992 [ipsi] and 1574 [contra] cells quantified). Scale bar, 50 μm. ****P < 0.0001, Unpaired t-test. (D) Paw withdrawal behaviors by light (score), von Frey filaments (threshold), and heat (latency) at 4 weeks after DTX or PBS injection (n = 7–9 rats). Data shown as mean ± SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35813063), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3364_human-pax2-affinity-purified-polyclonal-ab-22820241721190.jpg)
![Ablation of AAV-PdynP+ spinal dorsal horn (SDH) neurons does not affect behavioral responses evoked by the optical stimulation of A beta fibers, nociceptive mechanical force, and heat in rats. (A) Enhanced green fluorescent protein (EGFP) expression (green) in the L4 SDH at 4 weeks after intra-SDH microinjection of AAV-PdynP-DTR-EGFP (indicated by an upper schematic illustration). Colocalization of EGFP and PAX2 (red) in the SDH (indicated by arrowheads). Scale bar, 100 μm. The percentage of co-expressing neurons was quantified (n = 3 rats tested, 94 total EGFP+ cells quantified, and 339 total PAX2+ cells quantified). (B) Diphtheria toxin receptors (DTR) immunofluorescence in the SDH after administration of diphtheria toxin (DTX: 50 μg/kg, i.p., two injection 72 h apart) or PBS. Scale bar, 200 μm. (C) Immunohistochemical analysis and quantification of PAX2+ neurons in the superficial laminae of the ipsilateral and contralateral SDH at 4 weeks after DTX injection (n = 6 rats tested, PAX2+ neurons: 992 [ipsi] and 1574 [contra] cells quantified). Scale bar, 50 μm. ****P < 0.0001, Unpaired t-test. (D) Paw withdrawal behaviors by light (score), von Frey filaments (threshold), and heat (latency) at 4 weeks after DTX or PBS injection (n = 7–9 rats). Data shown as mean ± SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35813063), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3364_human-pax2-affinity-purified-polyclonal-ab-22820241723636.jpg)
![Western Blot: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of PAX2 expression in transfected 293T cell line by PAX2 monoclonal antibody (M01), clone 3C7.Lane 1: PAX2 transfected lysate(47.41 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-Western-Blot-H00005076-M01-img0006.jpg "Western Blot: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of PAX2 expression in transfected 293T cell line by PAX2 monoclonal antibody (M01), clone 3C7.Lane 1: PAX2 transfected lysate(47.41 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of monoclonal antibody to PAX2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 0.5 ug/ml.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-Immunohistochemistry-Paraffin-H00005076-M01-img0004.jpg)
![ELISA: Pax2 Antibody (3C7) [H00005076-M01] - Detection limit for recombinant GST tagged PAX2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-ELISA-H00005076-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Analysis in human kidney and prostate tissues using NBP2-88934 antibody. Corresponding PAX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pax2-Antibody-CL9469-Immunohistochemistry-Paraffin-NBP2-88934-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Analysis in human kidney and prostate tissues using NBP2-88934 antibody. Corresponding PAX2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Pax2 Antibody (CL9469) [NBP2-88934] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/Pax2-Antibody-CL9469-Western-Blot-NBP2-88934-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of EFO-21 cells using the Anti-PAX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Pax2-Antibody-CL9469-Immunocytochemistry-Immunofluorescence-NBP2-88934-img0001.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Pax2-Antibody-CL9469-Immunohistochemistry-Paraffin-NBP2-88934-img0002.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human kidney shows strong nuclear positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Pax2-Antibody-CL9469-Immunohistochemistry-Paraffin-NBP2-88934-img0003.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Pax2-Antibody-CL9469-Immunohistochemistry-Paraffin-NBP2-88934-img0004.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Pax2-Antibody-CL9469-Immunohistochemistry-Paraffin-NBP2-88934-img0005.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (CL9469) [NBP2-88934] - Staining of human renal cancer shows moderate nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/Pax2-Antibody-CL9469-Immunohistochemistry-Paraffin-NBP2-88934-img0006.jpg)
![Western Blot: Pax2 Antibody [NBP2-99013] - Lane A: K562 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:44 kDa](http://images.novusbio.com/fullsize/Pax2-Antibody-Western-Blot-NBP2-99013-img0008.jpg "Western Blot: Pax2 Antibody [NBP2-99013] - Lane A: K562 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:44 kDa")
![Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-99013] - Immunofluorescence staining of Pax2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Pax2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99013-img0001.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013] - Staining of human Pax2 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-99013-img0006.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013] - Staining of human Pax2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-99013-img0004.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013] - Staining of human Pax2 in human renal carcinoma with rabbit polyclonal antibody at 1:1000.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-99013-img0005.jpg)
![Immunoprecipitation: Pax2 Antibody [NBP2-99013] - Pax2 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 4 ul anti-Pax2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Pax2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 45 kDa](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunoprecipitation-NBP2-99013-img0007.jpg)
![Western Blot: Pax2 Antibody (2E4) [H00005076-M02] - PAX2 monoclonal antibody (M02), clone 2E4 Analysis of PAX2 expression in PC-12.](http://images.novusbio.com/fullsize/Pax2-Antibody-2E4-Western-Blot-H00005076-M02-img0003.jpg "Western Blot: Pax2 Antibody (2E4) [H00005076-M02] - PAX2 monoclonal antibody (M02), clone 2E4 Analysis of PAX2 expression in PC-12.")
![Sandwich ELISA: Pax2 Antibody (2E4) [H00005076-M02] - Detection limit for recombinant GST tagged PAX2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Pax2-Antibody-2E4-Sandwich-ELISA-H00005076-M02-img0002.jpg)
![Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of Pax2 on human kidney tissue lysates with Rabbit anti-Pax2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Pax2-Antibody-JJ082-08-Western-Blot-NBP2-67849-img0003.jpg "Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of Pax2 on human kidney tissue lysates with Rabbit anti-Pax2 antibody at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-Pax2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Pax2-Antibody-JJ082-08-Immunohistochemistry-Paraffin-NBP2-67849-img0005.jpg)
![Western Blot: Pax2 Antibody (JJ082-08) [NBP2-67849] - Western blot analysis of Pax2 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Pax2-Antibody-JJ082-08-Western-Blot-NBP2-67849-img0002.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of paraffin-embedded human kidney tissue using anti-Pax2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Pax2-Antibody-JJ082-08-Immunohistochemistry-Paraffin-NBP2-67849-img0001.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (JJ082-08) [NBP2-67849] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Pax2 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Pax2-Antibody-JJ082-08-Immunohistochemistry-Paraffin-NBP2-67849-img0004.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (PAX2/1104) [NBP2-79742] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Pax2 Antibody (PAX2/1104).](http://images.novusbio.com/fullsize/Pax2-Antibody-PAX2-1104-Immunohistochemistry-Paraffin-NBP2-79742-img0003.jpg "Immunohistochemistry-Paraffin: Pax2 Antibody (PAX2/1104) [NBP2-79742] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Pax2 Antibody (PAX2/1104).")
![Immunohistochemistry-Paraffin: Pax2 Antibody (PAX2/1104) [NBP2-79742] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Pax2 Antibody (PAX2/1104).](http://images.novusbio.com/fullsize/Pax2-Antibody-PAX2-1104-Immunohistochemistry-Paraffin-NBP2-79742-img0001.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody (PAX2/1104) [NBP2-79742] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Pax2 Antibody (PAX2/1104).](http://images.novusbio.com/fullsize/Pax2-Antibody-PAX2-1104-Immunohistochemistry-Paraffin-NBP2-79742-img0002.jpg)
![Protein Array: Pax2 Antibody (PAX2/1104) [NBP2-79742] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Pax2 Antibody (PAX2/1104). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Pax2-Antibody-PAX2-1104-Protein-Array-NBP2-79742-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-57700] - Staining of human cell line U-2 OS shows localization to nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57700-img0001.jpg "Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-57700] - Staining of human cell line U-2 OS shows localization to nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-57700] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-57700-img0003.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-57700] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-57700_rabbit-polyclonal-pax2-antibody-immunohistochemistry-paraffin-1142024122412..jpg)
![Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-99012] - Immunofluorescence staining of Pax2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Pax2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99012-img0001.jpg "Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-99012] - Immunofluorescence staining of Pax2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Pax2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.")
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99012] - Immunochemical staining Pax2 in human renal carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-99012-img0004.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99012] - Immunochemical staining Pax2 in human kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-99012-img0003.jpg)
![Western Blot: Pax2 Antibody [NBP2-99012] - Lane A: K562 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:44 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-99012_rabbit-polyclonal-pax2-antibody-western-blot-2102023101258..jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-33496] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-33496-img0005.jpg "Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-33496] - Staining of human epididymis shows high expression.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-33496] - Analysis in human epididymis and endometrium tissues using Anti-PAX2 antibody. Corresponding PAX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-33496-img0003.jpg)
![Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-33496] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/Pax2-Antibody-Immunohistochemistry-Paraffin-NBP2-33496-img0004.jpg)
![Western Blot: Pax2 Overexpression Lysate (Adult Normal) [NBP2-09796] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pax2.](http://images.novusbio.com/fullsize/Pax2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09796-img0001.jpg "Western Blot: Pax2 Overexpression Lysate (Adult Normal) [NBP2-09796] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pax2.")
![Western Blot: Pax2 Overexpression Lysate (Adult Normal) [NBP2-07925] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pax2.](http://images.novusbio.com/fullsize/Pax2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07925-img0001.jpg "Western Blot: Pax2 Overexpression Lysate (Adult Normal) [NBP2-07925] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pax2.")