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![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Staining of human cerebral cortex, kidney, liver and pancreas using Anti-Phenylalanine Hydroxylase antibody NBP2-48615 (A) shows similar protein distribution across tissues to independent antibody NBP1-80917 (B).](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0014.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-NBP2-48615-img0017.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0016.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0003.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0013.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0015.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human cerebral cortex, kidney, liver and pancreas using Anti-Phenylalanine Hydroxylase antibody NBP1-80917 (A) shows similar protein distribution across tissues to independent antibody NBP2-48615 (B).](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0021.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Analysis using Anti-PAH antibody NBP1-80917 (A) shows similar pattern to independent antibody NBP2-48615 (B).](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-NBP1-80917-img0013.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0025.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0019.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0022.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0023.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Staining of Rat Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-NBP1-52084-img0002.jpg "Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Staining of Rat Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-NBP1-52084-img0001.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [H00005053-B01P] - Analysis of PAH expression in human liver.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-H00005053-B01P-img0002.jpg "Western Blot: Phenylalanine Hydroxylase Antibody [H00005053-B01P] - Analysis of PAH expression in human liver.")
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [H00005053-B01P] - Analysis of purified antibody to PAH on formalin-fixed paraffin-embedded human kidney. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-H00005053-B01P-img0001.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [H00005053-B01P] - Analysis of PAH expression in transfected 293T cell line by PAH polyclonal antibody. Lane 1: PAH transfected lysate(49.72 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-H00005053-B01P-img0003.jpg)
![Immunohistochemistry: Phenylalanine Hydroxylase Antibody (17D10) [NBP3-26443] - Image of Phenylalanine Hydroxylase Antibody (17D10) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26443_rabbit-phenylalanine-hydroxylase-mab-17d10-262202415475628.jpg "Immunohistochemistry: Phenylalanine Hydroxylase Antibody (17D10) [NBP3-26443] - Image of Phenylalanine Hydroxylase Antibody (17D10) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunocytochemistry/Immunofluorescence: Phenylalanine Hydroxylase Antibody (17D10) [NBP3-26443] - Staining of MCF-7 cell with Phenylalanine Hydroxylase Antibody (17D10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 598-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26443_rabbit-phenylalanine-hydroxylase-mab-17d10-282202412493628.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody (S06-3H4) [NBP3-19516] - Western blot detection of Phenylalanine Hydroxylase in 3T3, C6, Rat Brain, K562 cell lysates using NBP3-19516 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-S06-3H4-Western-Blot-NBP3-19516-img0001.jpg "Western Blot: Phenylalanine Hydroxylase Antibody (S06-3H4) [NBP3-19516] - Western blot detection of Phenylalanine Hydroxylase in 3T3, C6, Rat Brain, K562 cell lysates using NBP3-19516 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.")
![SDS-Page: Recombinant Human Phenylalanine Hydroxylase Protein [H00005053-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Phenylalanine-Hydroxylase-Protein-SDS-Page-H00005053-P01-img0001.jpg "SDS-Page: Recombinant Human Phenylalanine Hydroxylase Protein [H00005053-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric) [NBP2-81206] - Samples were spiked with high concentrations of Human Phenylalanine Hydroxylase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81206_human-phenylalanine-hydroxylase-elisa-kit-colorimetric-132202416184862.jpg "ELISA: Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric) [NBP2-81206] - Samples were spiked with high concentrations of Human Phenylalanine Hydroxylase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Phenylalanine Hydroxylase ELISA Kit (Colorimetric) [NBP2-81206] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81206_human-phenylalanine-hydroxylase-elisa-kit-colorimetric-4320248595182.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [H00005053-D01P] - Analysis of PAH expression in transfected 293T cell line by PAH polyclonal antibody.Lane 1: PAH transfected lysate(51.80 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-H00005053-D01P-img0002.jpg "Western Blot: Phenylalanine Hydroxylase Antibody [H00005053-D01P] - Analysis of PAH expression in transfected 293T cell line by PAH polyclonal antibody.Lane 1: PAH transfected lysate(51.80 KDa).Lane 2: Non-transfected lysate.")