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![Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- Paf1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunohistochemistry-Paraffin-NB600-274-img0010.jpg "Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- Paf1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Paf1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunohistochemistry-Paraffin-NB600-274-img0009.jpg)
![Immunoprecipitation: PAF1 Antibody [NB600-274] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-274 Lot 2 used for IP at 3 ug/mg lysate. Paf1 was also immunoprecipitated by rabbit anti-Paf1 Lot 1 of the same product. For blotting immunoprecipitated Paf1, NB600-274 Lot 2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time 30 seconds.](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunoprecipitation-NB600-274-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PAF1 Antibody [NB600-274] - Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-274 (lot NB600-274-3) used for IP at 6 ug per reaction. Paf1 was also immunoprecipitated by a previous lot of this antibody (lot NB600-274-2) and rabbit anti-Paf1 antibody NB600-273. For blotting immunoprecipitated Paf1, NB600-274 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunoprecipitation-NB600-274-img0011.jpg)
![Western Blot: PAF1 Antibody [NB600-273] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPaf1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PAF1-Antibody-Western-Blot-NB600-273-img0006.jpg "Western Blot: PAF1 Antibody [NB600-273] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPaf1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PAF1 Antibody [NB600-273] - Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-273 used for IP at 3 ug per reaction. Paf1 was also immunoprecipitated by rabbit anti-Paf1 antibody NB600-274. For blotting immunoprecipitated Paf1, NB600-273 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunoprecipitation-NB600-273-img0007.jpg)
![Western Blot: PAF1 Antibody (3U5C4) [NBP3-16185] - Western blot analysis of extracts of various cell lines, using PAF1 Rabbit mAb (NBP3-16185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PAF1-Antibody-3U5C4-Western-Blot-NBP3-16185-img0002.jpg "Western Blot: PAF1 Antibody (3U5C4) [NBP3-16185] - Western blot analysis of extracts of various cell lines, using PAF1 Rabbit mAb (NBP3-16185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: PAF1 Antibody (3U5C4) [NBP3-16185] - Immunohistochemistry of paraffin-embedded human appendix using PAF1 Rabbit mAb (NBP3-16185) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PAF1-Antibody-3U5C4-Immunohistochemistry-Paraffin-NBP3-16185-img0001.jpg)
![Western Blot: PAF1 Antibody [NBP2-19675] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PAF1-Antibody-Western-Blot-NBP2-19675-img0001.jpg "Western Blot: PAF1 Antibody [NBP2-19675] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry: PAF1 Antibody [NBP2-19675] - PAF1 antibody detects Paf1 protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. PAF1 antibody (NBP2-19675) dilution: 1:200.](http://images.novusbio.com/fullsize/nbp2-19675_rabbit-polyclonal-paf1-antibody-910202420134082.jpg)
![Western Blot: PAF1 Antibody (SR2237) [NBP3-22163] - Western blot analysis using NBP3-22163 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22163_rabbit-paf1-mab-sr2237-5720231624721.jpg "Western Blot: PAF1 Antibody (SR2237) [NBP3-22163] - Western blot analysis using NBP3-22163 in K562 cell lysate.")
![Immunohistochemistry-Paraffin: PAF1 Antibody [NBP2-13724] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunohistochemistry-Paraffin-NBP2-13724-img0001.jpg "Immunohistochemistry-Paraffin: PAF1 Antibody [NBP2-13724] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.")
![Immunocytochemistry/Immunofluorescence: PAF1 Antibody [NBP2-54958] - Staining of human cell line MCF7 shows localization to nucleoplasm & cell junctions.](http://images.novusbio.com/fullsize/PAF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54958-img0001.jpg "Immunocytochemistry/Immunofluorescence: PAF1 Antibody [NBP2-54958] - Staining of human cell line MCF7 shows localization to nucleoplasm & cell junctions.")
