| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33221 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91189SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5935-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3839-SP | |||
![Knockdown Validated: MBD1 Antibody (100B272.1) [NB100-56537] - blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Mouse Anti-Human MBD1 Monoclonal Antibody (Catalog # NB100-56537) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Knockdown-Validated-NB100-56537-img0005.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: MBD1 Antibody (100B272.1) [NB100-56537] - blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Mouse Anti-Human MBD1 Monoclonal Antibody (Catalog # NB100-56537) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis using Azide and BSA Free version of NB100-56537. Jurkat cell lysate using anti-MBD1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Western-Blot-NB100-56537-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MBD1 Antibody (100B272.1) [NB100-56537] - NIH3T3 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MBD1 (100B272.1) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Immunocytochemistry-Immunofluorescence-NB100-56537-img0007.jpg)
![Immunohistochemistry-Paraffin: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of MBD1 (100B272.1) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and nuclear staining was observed.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Immunohistochemistry-Paraffin-NB100-56537-img0008.jpg)
![Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis of MBD1 in HeLa lysate using MBD1 antibody at 2 ug/ml (lane 1) and 1 ug/ml (lane 2).](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Western-Blot-NB100-56537-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MBD1 Antibody (100B272.1) [NB100-56537] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-MBD1 (100B272.1) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MBD1-Antibody-100B272-1-Immunocytochemistry-Immunofluorescence-NB100-56537-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MBD4 Antibody [NBP1-91189] - MBD4 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/MBD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91189-img0003.jpg "Immunocytochemistry/Immunofluorescence: MBD4 Antibody [NBP1-91189] - MBD4 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: MBD4 Antibody [NBP1-91189] - IHC analysis of MBD4 in human breast cancer cells using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/MBD4-Antibody-Immunohistochemistry-NBP1-91189-img0004.jpg)
![Western Blot: MBD4 Antibody [NBP2-24393] - analysis of MBD4 in Jurkat cell lysate (30ug) using anti-MBD4 antibody followed by goat anti-rabbit HRP conjugated secondary antibody (Cat. #NB7160). Image from verified customer review.](http://images.novusbio.com/fullsize/MBD4-Antibody-Western-Blot-NBP2-24393-img0002.jpg "Western Blot: MBD4 Antibody [NBP2-24393] - analysis of MBD4 in Jurkat cell lysate (30ug) using anti-MBD4 antibody followed by goat anti-rabbit HRP conjugated secondary antibody (Cat. #NB7160). Image from verified customer review.")
![Western Blot: MBD4 Antibody [NBP2-24393] - Analysis of MBD4 in HL60 cell lysate probed with MBD4 antibody.](http://images.novusbio.com/fullsize/MBD4-Antibody-Western-Blot-NBP2-24393-img0001.jpg)
![Western Blot: MBD2 Antibody [NB100-55415] - Staining of Jurkat cell lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MBD2-Antibody-Western-Blot-NB100-55415-img0002.jpg "Western Blot: MBD2 Antibody [NB100-55415] - Staining of Jurkat cell lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - Staining of paraffin embedded Human Skin with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-Paraffin-NB100-55415-img0007.jpg)
![Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - Staining of paraffin embedded Human Colon with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-Paraffin-NB100-55415-img0003.jpg)
![Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - Staining of paraffin embedded Human Adrenal Gland with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-Paraffin-NB100-55415-img0006.jpg)
![Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human cell line A-431Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/nbp3-21219_rabbit-mbd4-pab-45202315493245.jpg)
![Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.](http://images.novusbio.com/fullsize/MBD2-Antibody-Western-Blot-NB100-81657-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-81657] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MBD2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-NB100-81657-img0012.jpg)
![Knockdown Validated: MBD1 Antibody [NB100-56330] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human MBD1 Polyclonal Antibody (Catalog # NB100-56330) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/MBD1-Antibody-Knockdown-Validated-NB100-56330-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: MBD1 Antibody [NB100-56330] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human MBD1 Polyclonal Antibody (Catalog # NB100-56330) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MBD1 at approximately 60 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: MBD1 Antibody [NB100-56330] - Analysis of MBD1 in HeLa cell lysate with this antibody. A protein band with an approximate molecular weight of 55 kDa was detected.](http://images.novusbio.com/fullsize/MBD1-Antibody-Western-Blot-NB100-56330-img0002.jpg)
![Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MBD3-Antibody-9X1J7-Western-Blot-NBP3-16718-img0002.jpg "Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: MBD3 Antibody (9X1J7) [NBP3-16718] - Immunohistochemistry of paraffin-embedded human colon carcinoma using MBD3 Rabbit mAb (NBP3-16718) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MBD3-Antibody-9X1J7-Immunohistochemistry-Paraffin-NBP3-16718-img0001.jpg)
![Western Blot: MBD3 Antibody (9X1J7) [NBP3-16718] - Western blot analysis of extracts of various cell lines, using MBD3 Rabbit mAb (NBP3-16718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MBD3-Antibody-9X1J7-Western-Blot-NBP3-16718-img0003.jpg)
![Western Blot: MBD2 Antibody (SR1478) [NBP3-21856] - Western blot analysis using NBP3-21856 in A375 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21856_rabbit-mbd2-mab-sr1478-5720231552186.jpg "Western Blot: MBD2 Antibody (SR1478) [NBP3-21856] - Western blot analysis using NBP3-21856 in A375 cell lysate.")
![Western Blot: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of MBD5 expression in transfected 293T cell line by MBD5 monoclonal antibody (M01), clone 4A12-1B6.Lane 1: MBD5 transfected lysate(24.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MBD5-Antibody-4A12-1B6-Western-Blot-H00055777-M01-img0005.jpg "Western Blot: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of MBD5 expression in transfected 293T cell line by MBD5 monoclonal antibody (M01), clone 4A12-1B6.Lane 1: MBD5 transfected lysate(24.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Analysis of monoclonal antibody to MBD5 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MBD5-Antibody-4A12-1B6-Immunocytochemistry-Immunofluorescence-H00055777-M01-img0003.jpg)
![Sandwich ELISA: MBD5 Antibody (4A12-1B6) [H00055777-M01] - Detection limit for recombinant GST tagged MBD5 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MBD5-Antibody-4A12-1B6-Sandwich-ELISA-H00055777-M01-img0004.jpg)