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![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining in human cerebral cortex and skeletal muscle tissues using anti-MAG antibody. Corresponding MAG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP1-81817-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining in human cerebral cortex and skeletal muscle tissues using anti-MAG antibody. Corresponding MAG RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP1-81817-img0006.jpg)
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - MAG Antibody [NBP1-81817] - Image submitted by a verified customer review on mouse inner ear at 1:200 dilution.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-NBP1-81817-img0004.jpg)
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP1-81817] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP1-81817-img0005.jpg)
![Western Blot: MAG/Siglec-4a Antibody [NBP2-17201] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Western-Blot-NBP2-17201-img0003.jpg "Western Blot: MAG/Siglec-4a Antibody [NBP2-17201] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP2-17201] - Rat fore brain. MAG antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP2-17201-img0006.jpg)
![Western Blot: MAG/Siglec-4a Antibody [NBP2-17201] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Western-Blot-NBP2-17201-img0001.jpg)
![Immunohistochemistry-Frozen: MAG/Siglec-4a Antibody [NBP2-17201] - Frozen-sectioned adult mouse cerebellum. Green: MAG protein stained by MAG antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Frozen-NBP2-17201-img0005.jpg)
![Western Blot: MAG/Siglec-4a Antibody (3C7) [H00004099-M35] - Analysis of MAG expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-3C7-Western-Blot-H00004099-M35-img0006.jpg "Western Blot: MAG/Siglec-4a Antibody (3C7) [H00004099-M35] - Analysis of MAG expression in Jurkat (Cat # L017V1).")
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody (3C7) [H00004099-M35] - Analysis of monoclonal antibody to MAG on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-3C7-Immunohistochemistry-Paraffin-H00004099-M35-img0004.jpg)
![Sandwich ELISA: MAG/Siglec-4a Antibody (3C7) [H00004099-M35] - Detection limit for recombinant GST tagged MAG is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-3C7-Sandwich-ELISA-H00004099-M35-img0005.jpg)
![Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-278202416285214.jpg "Western Blot: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32557) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling MAG/Siglec-4a with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32557, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-29820241435217.jpg)
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-41) [NBP3-32557] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32557) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32557) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32557_rabbit-mag-siglec-4a-mab-psh02-41-298202414115423.jpg)
![Western Blot: MAG/Siglec-4a Antibody (PSH02-42) [NBP3-32558] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32558) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32558) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32558_rabbit-mag-siglec-4a-mab-psh02-42-278202415212494.jpg "Western Blot: MAG/Siglec-4a Antibody (PSH02-42) [NBP3-32558] - Western blot analysis of MAG/Siglec-4a on different lysates with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32558) at 1/2,000 dilution. Lane 1: Mouse brain(no heat) tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Rat brain(no heat) tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32558) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-42) [NBP3-32558] - Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32558) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32558) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32558_rabbit-mag-siglec-4a-mab-psh02-42-29820241435235.jpg)
![Immunohistochemistry: MAG/Siglec-4a Antibody (PSH02-42) [NBP3-32558] - Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling MAG/Siglec-4a with Rabbit anti-MAG/Siglec-4a antibody (NBP3-32558) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32558, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32558_rabbit-mag-siglec-4a-mab-psh02-42-298202413585594.jpg)
![Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859] - Analysis of extracts of SH-SY5Y cells, using MAG/Siglec-4a antibody (NBP3-16859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-8Q7K5-Western-Blot-NBP3-16859-img0002.jpg "Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859] - Analysis of extracts of SH-SY5Y cells, using MAG/Siglec-4a antibody (NBP3-16859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859] - Analysis of extracts of Mouse brain cells, using MAG/Siglec-4a antibody (NBP3-16859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-8Q7K5-Western-Blot-NBP3-16859-img0001.jpg)
![Western Blot: MAG/Siglec-4a Antibody (8Q7K5) [NBP3-16859] - Analysis of extracts of Rat brain cells, using MAG/Siglec-4a antibody (NBP3-16859) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-8Q7K5-Western-Blot-NBP3-16859-img0003.jpg)