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![Immunohistochemistry-Paraffin: LRWD1 Antibody [NBP1-85113] - Staining in human testis and prostate tissues using anti-LRWD1 antibody. Corresponding LRWD1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LRWD1-Antibody-Immunohistochemistry-Paraffin-NBP1-85113-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LRWD1 Antibody [NBP1-85113] - Staining in human testis and prostate tissues using anti-LRWD1 antibody. Corresponding LRWD1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LRWD1 Antibody [NBP1-85113] - Staining of human cell line U-2 OS shows localization to nucleus, nucleoli & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LRWD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85113-img0011.jpg)
![Immunohistochemistry-Paraffin: LRWD1 Antibody [NBP1-85113] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize/LRWD1-Antibody-Immunohistochemistry-Paraffin-NBP1-85113-img0005.jpg)
![Immunohistochemistry-Paraffin: LRWD1 Antibody [NBP1-85113] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/LRWD1-Antibody-Immunohistochemistry-Paraffin-NBP1-85113-img0006.jpg)
![Western Blot: LRWD1 Antibody [NBP1-03331] - Detection of Human and Mouse LRWD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, A459, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LRWD1 antibody NBP1-03331 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/LRWD1-Antibody-Western-Blot-NBP1-03331-img0002.jpg "Western Blot: LRWD1 Antibody [NBP1-03331] - Detection of Human and Mouse LRWD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, A459, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-LRWD1 antibody NBP1-03331 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: LRWD1 Antibody [NBP1-03331] - Detection of Human LRWD1 on HeLa whole cell lysate using NBP1-03331. LRWD1 was successfully immunoprecipitated by rabbit anti-LRWD1 antibody NBP1-03330.](http://images.novusbio.com/fullsize/LRWD1-Antibody-Western-Blot-NBP1-03331-img0001.jpg)
![Western Blot: LRWD1 Antibody [NBP3-03939] - Analysis of extracts of various cell lines, using LRWD1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/LRWD1-Antibody-Western-Blot-NBP3-03939-img0001.jpg "Western Blot: LRWD1 Antibody [NBP3-03939] - Analysis of extracts of various cell lines, using LRWD1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Western Blot: LRWD1 Antibody [NBP2-17176] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/LRWD1-Antibody-Western-Blot-NBP2-17176-img0001.jpg "Western Blot: LRWD1 Antibody [NBP2-17176] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: LRWD1 Overexpression Lysate (Adult Normal) [NBP2-05302] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LRWD1.](http://images.novusbio.com/fullsize/LRWD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05302-img0001.jpg "Western Blot: LRWD1 Overexpression Lysate (Adult Normal) [NBP2-05302] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LRWD1.")
![Immunocytochemistry/Immunofluorescence: LRWD1 Antibody [NBP2-97339] - Immunofluorescence staining of LRWD1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LRWD1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.](http://images.novusbio.com/fullsize/LRWD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97339-img0001.jpg "Immunocytochemistry/Immunofluorescence: LRWD1 Antibody [NBP2-97339] - Immunofluorescence staining of LRWD1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LRWD1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.")
![Immunoprecipitation: LRWD1 Antibody [NBP1-03330] - Detection of Human LRWD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-LRWD1 antibody NBP1-03330 used for IP at 3 mcg/mg lysate](http://images.novusbio.com/fullsize/LRWD1-Antibody-Immunoprecipitation-NBP1-03330-img0002.jpg "Immunoprecipitation: LRWD1 Antibody [NBP1-03330] - Detection of Human LRWD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-LRWD1 antibody NBP1-03330 used for IP at 3 mcg/mg lysate")