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![Immunohistochemistry: LRP2 Antibody (CD7D5) [NB110-96417] - Representative immunofluorescence stainings of control (Tead4m-z+) and Tead4 maternal/zygotic mutant (Tead4m-z-) embryos for Krt8 and Krt18 (16 cell stage embryos) and Lrp2 and Dab2 (32 to 64 cell stage embryos; Lrp2 and Dab2 were not detected in earlier stage embryos). n indicates total number of embryos analyzed. Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (elifesciences.org/articles/22906), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunocytochemistry-Immunofluorescence-NB110-96417-img0005.jpg "Immunohistochemistry: LRP2 Antibody (CD7D5) [NB110-96417] - Representative immunofluorescence stainings of control (Tead4m-z+) and Tead4 maternal/zygotic mutant (Tead4m-z-) embryos for Krt8 and Krt18 (16 cell stage embryos) and Lrp2 and Dab2 (32 to 64 cell stage embryos; Lrp2 and Dab2 were not detected in earlier stage embryos). n indicates total number of embryos analyzed. Scale bar: 25 um. Image collected and cropped by CiteAb from the following publication (elifesciences.org/articles/22906), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunohistochemistry-Paraffin-NB110-96417-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - Immunofluorescence staining of Megalin in the brush border of the proximal tubule.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunocytochemistry-Immunofluorescence-NB110-96417-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - Hek293 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-LRP2 Antibody (CD7D5) NB110-96417 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunocytochemistry-Immunofluorescence-NB110-96417-img0007.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of LRP2 [CD7D5] antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunohistochemistry-Paraffin-NB110-96417-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: LRP2 Antibody (CD7D5) [NB110-96417] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Saponin. The cells were incubated with anti-LRP2 (CD7D5) at 20 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunocytochemistry-Immunofluorescence-NB110-96417-img0004.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody (CD7D5) [NB110-96417] - Staining on human kidney at 1:100, overnight incubation at 4C. Image from a review by a confirmed customer.](http://images.novusbio.com/fullsize/LRP2-Antibody-CD7D5-Immunohistochemistry-Paraffin-NB110-96417-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417] - ICM & TE progenitors show loss of responsiveness to Hippo signaling manipulation at the same time as they loose responsiveness to positional changes. (D) Dox-inducible DN Lats2-IRES-mCherry transgenic embryos imaged before Dox treatment (top panel) & the same embryo was imaged following 24 hr of Dox live (middle panel) & fixed/stained for lineage markers (bottom panel). A representative embryo is shown for each stage. Live mCherry is shown as an extended focus image, IF stainings shown as single plane images. mCherry positive ICMs in mosaic transgenic embryos circled with a dotted line. Arrow points to a rare ICM cell in a 64 cell stage-induced embryo with weak Cdx2 expression, which also co-expressed an ICM marker. Scale bar: 25 µm. n indicates number of transgenic embryos analyzed. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/22906), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96417_mouse-monoclonal-lrp2-antibody-cd7d5-31020241621834.jpg)
![Immunocytochemistry/ Immunofluorescence: LRP2 Antibody (CD7D5) - Azide and BSA Free [NB110-96417] - Impairment of autophagy in Atg7-deficient renal proximal tubular cells. (A) Decrease of Atg7 & increase of LC3-I in 2-month-old Atg7flox/flox;KAP-Cre+ mouse kidney. Atg7 & LC3 Western blot (LC3-I/unconjugated LC3 & LC3-II/lipidated LC3) using whole kidney lysate of Atg7flox/flox;KAP-Cre+ mice. As a control, Atg7flox/flox mouse kidney was employed. The intensity of each band of Atg7 & beta -actin was estimated by densitometry. The ratio of Atg7 to beta -actin was calculated. The amount of Atg7-positive signals in the Atg7flox/flox;KAP-Cre+ mice kidney was about 63% lower than that in the Atg7flox/flox mice kidney (n = 3). Note that LC3-I was increased in the Atg7flox/flox;KAP-Cre+ mouse kidney. (B) The massive accumulation of p62 in kidneys of 2-month old Atg7flox/flox;KAP-Cre+ mouse. The cortico-medullary region of each kidney in 2 month-old Atg7flox/flox;KAP-Cre+ mouse & Atg7flox/flox mouse was recognized with anti-p62 antibody (red). As a marker of renal proximal tubular cells, megalin (green) was employed. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). (C) Quantification of p62 positive area of 2-month-old Atg7flox/flox;KAP-Cre+ (n = 5) & Atg7flox/flox kidney (n = 4, *** p < 0.01). Data in graphs are expressed as the mean ± SEM. Statistical analyses were performed using a student’s t-test. (D) Age-dependent accumulation of p62 (red) in the Atg7flox/flox;KAP-Cre+ mouse kidney in 1-, 2-, 6-, & 9-month-old Atg7flox/flox;KAP-Cre+ (lower) & Atg7flox/flox (upper) mouse kidney. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31881660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-96417_mouse-monoclonal-lrp2-antibody-cd7d5-310202416205167.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Analysis in human parathyroid gland and placenta tissues. Corresponding LRP2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Analysis in human parathyroid gland and placenta tissues. Corresponding LRP2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0012.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0009.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0010.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0011.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0013.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody (545818) [Unconjugated] [MAB95781] - Megalin/LRP2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Megalin/LRP2 Monoclonal Antibody (Catalog # MAB95781) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membrane of epithelial cells in convoluted tubules.](http://images.novusbio.com/fullsize/LRP2-Antibody-545818-Unconjugated-Immunohistochemistry-Paraffin-MAB95781-img0001.jpg "Immunohistochemistry-Paraffin: LRP2 Antibody (545818) [Unconjugated] [MAB95781] - Megalin/LRP2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Megalin/LRP2 Monoclonal Antibody (Catalog # MAB95781) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membrane of epithelial cells in convoluted tubules.")
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP1-85292] - Staining in human kidney and skeletal muscle tissues.. Corresponding LRP2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-85292-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LRP2 Antibody [NBP1-85292] - Staining in human kidney and skeletal muscle tissues.. Corresponding LRP2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LRP2 Antibody [NBP1-85292] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-85292-img0010.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP1-85292] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-85292-img0011.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP1-85292] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-85292-img0012.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP1-85292] - Staining of human thyroid gland shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-85292-img0014.jpg)
![Western Blot: LRP2 Antibody (PSH02-91) [NBP3-32545] - Western blot analysis of LRP2 on human kidney tissue lysates with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32545) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-278202416285222.jpg "Western Blot: LRP2 Antibody (PSH02-91) [NBP3-32545] - Western blot analysis of LRP2 on human kidney tissue lysates with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32545) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LRP2 Antibody (PSH02-91) [NBP3-32545] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling LRP2 with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32545, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-29820241492681.jpg)
![Immunohistochemistry: LRP2 Antibody (PSH02-91) [NBP3-32545] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LRP2 antibody (NBP3-32545) at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32545) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32545_rabbit-lrp2-mab-psh02-91-29820241492624.jpg)
![Western Blot: LRP2 Antibody (PSH04-52) [NBP3-32546] - Western blot analysis of LRP2 on different lysates with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32546) at 1/2,000 dilution was used in TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32546_rabbit-lrp2-mab-psh04-52-27820241628527.jpg "Western Blot: LRP2 Antibody (PSH04-52) [NBP3-32546] - Western blot analysis of LRP2 on different lysates with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat kidney tissue lysate Lane 4: Mouse skeletal muscle tissue lysate (negative) Lane 5: Rat skeletal muscle tissue lysate (negative) Lysates/proteins at 40 ug/Lane. Predicted band size: 522 kDa Observed band size: 600 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32546) at 1/2,000 dilution was used in TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LRP2 Antibody (PSH04-52) [NBP3-32546] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LRP2 antibody (NBP3-32546) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32546) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32546_rabbit-lrp2-mab-psh04-52-298202414262677.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP3-21426] - Immunochemical staining of human LRP2 in human kidney with NBP3-21426 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-21426_rabbit-lrp2-pab-1262024149343.png)
![Western Blot: LRP2 Antibody (SR1448) [NBP3-21611] - Western blot analysis using NBP3-21611 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21611_rabbit-lrp2-mab-sr1448-57202317161814.jpg "Western Blot: LRP2 Antibody (SR1448) [NBP3-21611] - Western blot analysis using NBP3-21611 in 293T cell lysate.")
![ELISA: Human LRP2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40031] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40031_human-lrp2-ready-to-use-elisa-kit-colorimetric-2110202415393786.png "ELISA: Human LRP2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40031] - Standard Curve Reference")
![ELISA: Human LRP2 ELISA Kit (Colorimetric) [NBP3-40030] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40030_human-lrp2-elisa-kit-colorimetric-2110202415393735.png "ELISA: Human LRP2 ELISA Kit (Colorimetric) [NBP3-40030] - Standard Curve Reference")