| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2199-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2058-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2058-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87793-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB21992-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2199-SP | |||
![Immunocytochemistry/Immunofluorescence: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793] - Staining of human cell line RT4 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Legumain-Asparaginyl-Endopeptidase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87793-img0008.jpg "Immunocytochemistry/Immunofluorescence: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793] - Staining of human cell line RT4 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Legumain-Asparaginyl-Endopeptidase-Antibody-Immunohistochemistry-Paraffin-NBP1-87793-img0012.jpg)
![Immunohistochemistry-Paraffin: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793] - Staining of human tonsil shows strong granular cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Legumain-Asparaginyl-Endopeptidase-Antibody-Immunohistochemistry-Paraffin-NBP1-87793-img0009.jpg)
![Immunohistochemistry-Paraffin: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793] - Staining of human gastrointestinal shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Legumain-Asparaginyl-Endopeptidase-Antibody-Immunohistochemistry-Paraffin-NBP1-87793-img0010.jpg)
![Immunohistochemistry-Paraffin: Legumain/Asparaginyl Endopeptidase Antibody [NBP1-87793] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Legumain-Asparaginyl-Endopeptidase-Antibody-Immunohistochemistry-Paraffin-NBP1-87793-img0011.jpg)
![Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (JE35-56) [NBP3-32528] - Western blot analysis of Legumain/Asparaginyl Endopeptidase on different lysates with Rabbit anti-Legumain/Asparaginyl Endopeptidase antibody (NBP3-32528) at 1/1,000 dilution. Lane 1: HT-29 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: Neuro-2a cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: RAW264.7 cell lysate (20 ug/Lane) Lane 8: Mouse kidney tissue lysate (40 ug/Lane) Lane 9: Mouse placenta tissue lysate (40 ug/Lane) Lane 10: Rat kidney tissue lysate (40 ug/Lane) Lane 11: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 49 kDa Observed band size: 36/46/56 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32528) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32528_rabbit-legumain-asparaginyl-endopeptidase-mab-je35-56-278202415242623.jpg "Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (JE35-56) [NBP3-32528] - Western blot analysis of Legumain/Asparaginyl Endopeptidase on different lysates with Rabbit anti-Legumain/Asparaginyl Endopeptidase antibody (NBP3-32528) at 1/1,000 dilution. Lane 1: HT-29 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: Neuro-2a cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: RAW264.7 cell lysate (20 ug/Lane) Lane 8: Mouse kidney tissue lysate (40 ug/Lane) Lane 9: Mouse placenta tissue lysate (40 ug/Lane) Lane 10: Rat kidney tissue lysate (40 ug/Lane) Lane 11: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 49 kDa Observed band size: 36/46/56 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32528) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Legumain/Asparaginyl Endopeptidase Antibody (JE35-56) [NBP3-32528] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Legumain/Asparaginyl Endopeptidase antibody (NBP3-32528) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32528) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32528_rabbit-legumain-asparaginyl-endopeptidase-mab-je35-56-298202414161817.jpg)
![Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (6X5U6) [NBP3-16645] - Western blot analysis of extracts of various cell lines, using Legumain/Asparaginyl Endopeptidase (LGMN) Rabbit mAb (NBP3-16645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Legumain-Asparaginyl-Endopeptidase-Antibody-6X5U6-Western-Blot-NBP3-16645-img0001.jpg "Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (6X5U6) [NBP3-16645] - Western blot analysis of extracts of various cell lines, using Legumain/Asparaginyl Endopeptidase (LGMN) Rabbit mAb (NBP3-16645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (6X5U6) [NBP3-16645] - Western blot analysis of various lysates using Legumain/Asparaginyl Endopeptidase Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16645_rabbit-legumain-asparaginyl-endopeptidase-mab-6x5u6-52202511411467.jpg)
![Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (6X5U6) [Legumain/Asparaginyl Endopeptidase] - Western blot analysis of various lysates using Legumain/Asparaginyl Endopeptidase Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16645_rabbit-legumain-asparaginyl-endopeptidase-mab-6x5u6-52202517352721.jpg)
![Western Blot: Legumain Overexpression Lysate (Adult Normal) [NBL1-12508] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Legumain.](http://images.novusbio.com/fullsize/Legumain-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12508-img0002.jpg "Western Blot: Legumain Overexpression Lysate (Adult Normal) [NBL1-12508] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Legumain.")
![Western Blot: Legumain Overexpression Lysate (Adult Normal) [NBL1-12508] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for LGMN](http://images.novusbio.com/fullsize/Legumain-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12508-img0001.jpg)
![Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (S06-7G1) [NBP3-19561] - Western blot detection of Legumain/Asparaginyl Endopeptidase in Jurkat, Hela cell lysates using NBP3-19561 (1:1000 diluted). Predicted band size: 49kDa. Observed band size: 56, 46, 37kDa.](http://images.novusbio.com/fullsize/Legumain-Asparaginyl-Endopeptidase-Antibody-S06-7G1-Western-Blot-NBP3-19561-img0001.jpg "Western Blot: Legumain/Asparaginyl Endopeptidase Antibody (S06-7G1) [NBP3-19561] - Western blot detection of Legumain/Asparaginyl Endopeptidase in Jurkat, Hela cell lysates using NBP3-19561 (1:1000 diluted). Predicted band size: 49kDa. Observed band size: 56, 46, 37kDa.")