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![Western Blot: Cytokeratin 4 Antibody (6H9R6) [NBP3-15243] - Western blot analysis of extracts of various cell lines, using Cytokeratin 4 (KRT4) Rabbit mAb (NBP3-15243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-6H9R6-Western-Blot-NBP3-15243-img0004.jpg "Western Blot: Cytokeratin 4 Antibody (6H9R6) [NBP3-15243] - Western blot analysis of extracts of various cell lines, using Cytokeratin 4 (KRT4) Rabbit mAb (NBP3-15243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry: Cytokeratin 4 Antibody (6H9R6) [NBP3-15243] - Immunofluorescence analysis of mouse large intestine using Cytokeratin 4 (KRT4) Rabbit mAb (NBP3-15243) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-6H9R6-Immunohistochemistry-NBP3-15243-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (6H9R6) [NBP3-15243] - Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using Cytokeratin 4 (KRT4) Rabbit mAb (NBP3-15243) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-6H9R6-Immunohistochemistry-Paraffin-NBP3-15243-img0001.jpg)
![Immunohistochemistry: Cytokeratin 4 Antibody (6H9R6) [NBP3-15243] - Immunofluorescence analysis of rat rectum using Cytokeratin 4 (KRT4) Rabbit mAb (NBP3-15243) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-6H9R6-Immunohistochemistry-NBP3-15243-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 4 Antibody (6H9R6) [Cytokeratin 4] - Confocal imaging of human tonsil tissue using Cytokeratin 4 Rabbit mAb . DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer (pH 6.0) before commencing with IF staining protocol.](http://images.novusbio.com/fullsize/nbp3-15243_rabbit-cytokeratin-4-mab-6h9r6-52202517162357.jpg)
![Immunohistochemistry: Cytokeratin 4 Antibody (6H9R6) [Cytokeratin 4] - Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using Cytokeratin 4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15243_rabbit-cytokeratin-4-mab-6h9r6-52202517184522.jpg)
![Immunohistochemistry: Cytokeratin 4 Antibody (6H9R6) [Cytokeratin 4] - Immunohistochemistry analysis of paraffin-embedded human breast tissue using Cytokeratin 4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15243_rabbit-cytokeratin-4-mab-6h9r6-5220251728494.jpg)
![Immunohistochemistry: Cytokeratin 4 Antibody (6H9R6) [Cytokeratin 4] - Immunohistochemistry analysis of paraffin-embedded human esophagus tissue using Cytokeratin 4 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15243_rabbit-cytokeratin-4-mab-6h9r6-52202517235752.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Staining of Cytokeratin 4 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunocytochemistry-Immunofluorescence-NBP2-67706-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Staining of Cytokeratin 4 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunohistochemistry-Paraffin-NBP2-67706-img0009.jpg)
![Flow Cytometry: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Analysis of A431 cells with Cytokeratin 4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Flow-Cytometry-NBP2-67706-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Staining Cytokeratin 4 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunocytochemistry-Immunofluorescence-NBP2-67706-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Staining Cytokeratin 4 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunocytochemistry-Immunofluorescence-NBP2-67706-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Staining Cytokeratin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunocytochemistry-Immunofluorescence-NBP2-67706-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunohistochemistry-Paraffin-NBP2-67706-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 4 (washed with PBS. And then probed with the primary antibodies Cytokeratin 4 ( red) at 1/200 dilution and Vimentin (green) at 1/200 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunohistochemistry-Paraffin-NBP2-67706-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (SN74-03) [NBP2-67706] - Analysis of paraffin-embedded human esophagus tissue using anti-Cytokeratin 4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-SN74-03-Immunohistochemistry-Paraffin-NBP2-67706-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797] - Immunofluorescence Analysis of A549 cells labeling KRT4 with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-KRT4-2804-Immunocytochemistry-Immunofluorescence-NBP3-07797-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797] - Immunofluorescence Analysis of A549 cells labeling KRT4 with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).")
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804).](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-KRT4-2804-Immunohistochemistry-Paraffin-NBP3-07797-img0004.jpg)
![Flow Cytometry: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-KRT4-2804-Flow-Cytometry-NBP3-07797-img0002.jpg)
![Protein Array: Cytokeratin 4 Antibody (KRT4/2804) [NBP3-07797] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804).](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-KRT4-2804-Protein-Array-NBP3-07797-img0001.jpg)
![Western Blot: Cytokeratin 4 Antibody [NBP1-33412] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-Western-Blot-NBP1-33412-img0005.jpg "Western Blot: Cytokeratin 4 Antibody [NBP1-33412] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody [NBP1-33412] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: KRT4 protein stained by Cytokeratin 4 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33412-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody [NBP1-33412] - Paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-33412-img0004.jpg)
![Western Blot: Cytokeratin 4 Antibody [NBP1-33412] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 4 antibody (NBP1-33412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33412_rabbit-polyclonal-cytokeratin-4-antibody-910202420122074.jpg)
![Western Blot: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - KRT4 monoclonal antibody (M01), clone 5H5 Analysis of KRT4 expression in A-431.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-5H5-Western-Blot-H00003851-M01-img0010.jpg "Western Blot: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - KRT4 monoclonal antibody (M01), clone 5H5 Analysis of KRT4 expression in A-431.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - Analysis of monoclonal antibody to KRT4 on A-431 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-5H5-Immunocytochemistry-Immunofluorescence-H00003851-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - Analysis of monoclonal antibody to KRT4 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-5H5-Immunohistochemistry-Paraffin-H00003851-M01-img0008.jpg)
![ELISA: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - Detection limit for recombinant GST tagged KRT4 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-5H5-ELISA-H00003851-M01-img0011.jpg)
![Western Blot: Cytokeratin 4 Antibody [NBP2-33984] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-Western-Blot-NBP2-33984-img0003.jpg "Western Blot: Cytokeratin 4 Antibody [NBP2-33984] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody [NBP2-33984] - Staining of human cell line HaCaT shows localization to intermediate filaments.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33984-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody [NBP2-33984] - Staining of human oral mucosa shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-33984-img0001.jpg)
![Western Blot: Cytokeratin 4 Antibody [NBP1-33592] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-Western-Blot-NBP1-33592-img0003.jpg "Western Blot: Cytokeratin 4 Antibody [NBP1-33592] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody [NBP1-33592] - Paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-33592-img0002.jpg)
![Western Blot: Cytokeratin 4 Antibody [NBP1-33592] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cytokeratin 4 antibody (NBP1-33592) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33592_rabbit-polyclonal-cytokeratin-4-antibody-9102024201467.jpg)
![Immunohistochemistry: Cytokeratin 4 Antibody (RM355)[NBP3-18825]Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CK-4 rabbit monoclonal antibody (Clone RM355) at a 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-18825_rabbit-cytokeratin-4-mab-rm355-16112023130189.png "Immunohistochemistry: Cytokeratin 4 Antibody (RM355)[NBP3-18825]Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-CK-4 rabbit monoclonal antibody (Clone RM355) at a 1:1000 dilution.")
![SDS-Page: Cytokeratin 4 Recombinant Protein [H00003851-P01]](http://images.novusbio.com/fullsize/Cytokeratin-4-Recombinant-Protein-SDS-Page-H00003851-P01-img0001.jpg "SDS-Page: Cytokeratin 4 Recombinant Protein [H00003851-P01]")
![Western Blot: Cytokeratin 4 Overexpression Lysate (Adult Normal) [NBP2-10820] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cytokeratin 4.](http://images.novusbio.com/fullsize/Cytokeratin-4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10820-img0001.jpg "Western Blot: Cytokeratin 4 Overexpression Lysate (Adult Normal) [NBP2-10820] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cytokeratin 4.")
![ELISA: Human Cytokeratin 4 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39684] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39684_human-cytokeratin-4-ready-to-use-elisa-kit-colorimetric-2110202415305291.png "ELISA: Human Cytokeratin 4 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39684] - Standard Curve Reference")
![ELISA: Human Cytokeratin 4 ELISA Kit (Colorimetric) [NBP3-39683] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39683_human-cytokeratin-4-elisa-kit-colorimetric-211020241534395.png "ELISA: Human Cytokeratin 4 ELISA Kit (Colorimetric) [NBP3-39683] - Standard Curve Reference")