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![Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP2-42864-img0011.jpg "Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HRAS Antibody [NBP2-42864] - Analysis of paraformaldehyde-fixed HeLa, using H-Ras antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42864-img0001.jpg)
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864] - Mouse intestine. H-RAS antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP2-42864-img0003.jpg)
![Western Blot: HRAS Antibody [NBP2-42864] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP2-42864-img0006.jpg)
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864] - Mouse intestine. H-RAS antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP2-42864-img0002.jpg)
![Western Blot: HRAS Antibody [NBP2-42864] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-42864_rabbit-polyclonal-hras-antibody-western-blot-2022024123352..jpg)
![Western Blot: HRAS Antibody [NBP2-42864] -Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-42864_rabbit-polyclonal-hras-antibody-western-blot-2022024123527..jpg)
![Western Blot: HRAS Antibody [NBP2-42864] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-42864_rabbit-polyclonal-hras-antibody-western-blot-2022024123656..jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0006.jpg "Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0004.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY vH RAS.](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0005.jpg)
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (10ug) from 8 Human tissue by using HRAS monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0007.jpg)
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0002.jpg)
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0003.jpg)
![Immunofluorescence: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/HRAS-Antibody-OTI1D9-Immunofluorescence-NBP2-45745-img0001.jpg)
![Western Blot: HRAS Antibody [NBP3-04117] - Analysis of extracts of various cell lines, using HRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP3-04117-img0003.jpg "Western Blot: HRAS Antibody [NBP3-04117] - Analysis of extracts of various cell lines, using HRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.")
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP3-04117] - Paraffin-embedded human gastric cancer using HRAS antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP3-04117-img0002.jpg)
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP3-04117] - Paraffin-embedded human gastric using HRAS antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP3-04117-img0001.jpg)
![Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818] - Western blot analysis of extracts of various cell lines, using HRAS antibody (NBP3-15818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/HRAS-Antibody-4Q7M5-Western-Blot-NBP3-15818-img0002.jpg "Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818] - Western blot analysis of extracts of various cell lines, using HRAS antibody (NBP3-15818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: HRAS Antibody (4Q7M5) [NBP3-15818] - Immunohistochemistry of paraffin-embedded human breast cancer using HRAS antibody (NBP3-15818) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HRAS-Antibody-4Q7M5-Immunohistochemistry-Paraffin-NBP3-15818-img0001.jpg)
![Western Blot: HRAS Antibody (SR1327) [NBP3-21644] - Western blot analysis MCF7 cell lysate using NBP3-21644.](http://images.novusbio.com/fullsize/nbp3-21644_rabbit-hras-mab-sr1327-672023106249.jpg "Western Blot: HRAS Antibody (SR1327) [NBP3-21644] - Western blot analysis MCF7 cell lysate using NBP3-21644.")
![Western Blot: HRAS Antibody (JE42-15) [NBP3-32413] - Western blot analysis of HRAS on different lysates with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: Jurkat cell lysate (40 ug/Lane) Lane 3: NIH/3T3 cell lysate (40 ug/Lane) Lane 4: PC-12 cell lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (10 ug/Lane) Lane 6: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32413) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32413_rabbit-hras-mab-je42-15-278202415295577.jpg "Western Blot: HRAS Antibody (JE42-15) [NBP3-32413] - Western blot analysis of HRAS on different lysates with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 ug/Lane) Lane 2: Jurkat cell lysate (40 ug/Lane) Lane 3: NIH/3T3 cell lysate (40 ug/Lane) Lane 4: PC-12 cell lysate (40 ug/Lane) Lane 5: Rat brain tissue lysate (10 ug/Lane) Lane 6: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32413) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HRAS Antibody (JE42-15) [NBP3-32413] - Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-HRAS antibody (NBP3-32413) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32413) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32413_rabbit-hras-mab-je42-15-298202414161896.jpg)
![Western Blot: HRAS Antibody [NBP3-04486] - Analysis of extracts of mouse brain, using HRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP3-04486-img0002.jpg "Western Blot: HRAS Antibody [NBP3-04486] - Analysis of extracts of mouse brain, using HRAS antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit")
![Immunocytochemistry/Immunofluorescence: HRAS Antibody [NBP3-04486] - Analysis of HeLa cells using HRAS antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04486-img0001.jpg)
![Western Blot: HRAS Antibody [NBP2-99921] - Anti-HRAS rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP2-99921-img0004.jpg "Western Blot: HRAS Antibody [NBP2-99921] - Anti-HRAS rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa")
![Immunoprecipitation: HRAS Antibody [NBP2-99921] - HRAS was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg MCF-7 Whole Cell Lysate 2 ul anti-HRAS rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HRAS rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunoprecipitation-NBP2-99921-img0003.jpg)
![Western Blot: vH RAS Antibody (H-Ras-03) [NB110-68799] - Western Blotting analysis (reducing conditions) of vH RAS Antibody (H-Ras-03) in whole cell lysate using anti-human vH RAS Antibody (H-Ras-03). Lane 1: HeLa human cervix carcinoma cell line Lane 2: K567 human leukemia cell line Lane 3: RAJI human Burkitt lymphoma cell line.](http://images.novusbio.com/fullsize/vH-RAS-Antibody-H-Ras-03-Western-Blot-NB110-68799-img0001.jpg "Western Blot: vH RAS Antibody (H-Ras-03) [NB110-68799] - Western Blotting analysis (reducing conditions) of vH RAS Antibody (H-Ras-03) in whole cell lysate using anti-human vH RAS Antibody (H-Ras-03). Lane 1: HeLa human cervix carcinoma cell line Lane 2: K567 human leukemia cell line Lane 3: RAJI human Burkitt lymphoma cell line.")
![SDS-Page: Recombinant Human HRAS Protein [NBP1-50831] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-vH-RAS-Protein-SDS-Page-NBP1-50831-img0002.jpg "SDS-Page: Recombinant Human HRAS Protein [NBP1-50831] - 15% SDS-PAGE (3ug)")
![Western Blot: vH RAS Overexpression Lysate (Adult Normal) [NBP2-11106] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for vH RAS.](http://images.novusbio.com/fullsize/vH-RAS-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11106-img0001.jpg "Western Blot: vH RAS Overexpression Lysate (Adult Normal) [NBP2-11106] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for vH RAS.")