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![Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Western blot analysis of extracts of various cell lines, using Glycerol Kinase (GK) antibody (NBP3-15687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-8S4T8-Western-Blot-NBP3-15687-img0003.jpg "Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Western blot analysis of extracts of various cell lines, using Glycerol Kinase (GK) antibody (NBP3-15687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Immunohistochemistry of paraffin-embedded mouse testis using Glycerol Kinase Rabbit mAb (NBP3-15687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-8S4T8-Immunohistochemistry-Paraffin-NBP3-15687-img0002.jpg)
![Western Blot: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Western blot analysis of extracts of various cell lines, using Glycerol Kinase (GK) antibody (NBP3-15687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-8S4T8-Western-Blot-NBP3-15687-img0004.jpg)
![Immunohistochemistry-Paraffin: Glycerol Kinase Antibody (8S4T8) [NBP3-15687] - Immunohistochemistry of paraffin-embedded rat kidney using Glycerol Kinase Rabbit mAb (NBP3-15687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-8S4T8-Immunohistochemistry-Paraffin-NBP3-15687-img0001.jpg)
![Western Blot: Glycerol kinase Antibody [NBP1-57033] - U937 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Glycerol-kinase-Antibody-Western-Blot-NBP1-57033-img0002.jpg "Western Blot: Glycerol kinase Antibody [NBP1-57033] - U937 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Glycerol Kinase Antibody [H00002710-B01P] - Analysis of GK expression in human placenta.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-Western-Blot-H00002710-B01P-img0002.jpg "Western Blot: Glycerol Kinase Antibody [H00002710-B01P] - Analysis of GK expression in human placenta.")
![Immunohistochemistry-Paraffin: Glycerol Kinase Antibody [H00002710-B01P] - Analysis of purified antibody to GK on formalin-fixed paraffin-embedded human kidney. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-Immunohistochemistry-Paraffin-H00002710-B01P-img0001.jpg)
![Western Blot: Glycerol Kinase Antibody [H00002710-B01P] - Analysis of GK expression in transfected 293T cell line by GK polyclonal antibody. Lane 1: GK transfected lysate(57.64 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-Western-Blot-H00002710-B01P-img0003.jpg)
![Western Blot: Glycerol Kinase Antibody [NBP1-78018] - Glycerol Kinase antibody was used to detect purified Glycerol Kinase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (block 4C). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/blocking buffer 1 hr RT). Images were collected using the VersaDoc System.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-Western-Blot-NBP1-78018-img0007.jpg "Western Blot: Glycerol Kinase Antibody [NBP1-78018] - Glycerol Kinase antibody was used to detect purified Glycerol Kinase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (block 4C). Detection shown was using Dylight 488 conjugated Donkey anti goat (1:10K in TBS/blocking buffer 1 hr RT). Images were collected using the VersaDoc System.")
![Western Blot: Glycerol Kinase Antibody [NBP1-78018] - Analysis using Biotin conjugate of NBP1-78018. Lane 1: Glycerol Kinase. Load: 50 ng per lane. Primary antibody: Glycerol Kinase Antibody Biotin Conjugated at 1:1000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Glycerol Kinase.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-Western-Blot-NBP1-78018-img0005.jpg)
![Western Blot: Glycerol Kinase Antibody [NBP1-78018] - Both the antiserum and IgG fractions of Glycerol Kinase antibody) are shown to detect the 128,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:4,000 dilution of the primary antibody is used followed by detection using HRP Rabbit-a-Goat IgG [H&L] diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-Western-Blot-NBP1-78018-img0006.jpg)
![Western Blot: Glycerol Kinase Antibody (SR1882) [NBP3-22322] - Western blot analysis using NBP3-22322 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22322_rabbit-glycerol-kinase-mab-sr1882-57202317161885.jpg "Western Blot: Glycerol Kinase Antibody (SR1882) [NBP3-22322] - Western blot analysis using NBP3-22322 in HepG2 cell lysate.")
![Western Blot: Glycerol Kinase Antibody [H00002710-D01P] - Analysis of GK expression in human liver.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-Western-Blot-H00002710-D01P-img0001.jpg "Western Blot: Glycerol Kinase Antibody [H00002710-D01P] - Analysis of GK expression in human liver.")
![Western Blot: Glycerol Kinase Antibody [H00002710-D01P] - Analysis of GK expression in transfected 293T cell line by GK polyclonal antibody.Lane 1: GK transfected lysate(57.50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-Western-Blot-H00002710-D01P-img0002.jpg)
![Immunohistochemistry-Paraffin: Glycerol Kinase Antibody [NBP2-32561] - GK2 Antibody [NBP2-32561] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-Immunohistochemistry-Paraffin-NBP2-32561-img0007.jpg "Immunohistochemistry-Paraffin: Glycerol Kinase Antibody [NBP2-32561] - GK2 Antibody [NBP2-32561] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.")
![Western Blot: Glycerol Kinase Antibody [NBP3-09340] - Western blot analysis of Glycerol Kinase in Mouse Stomach lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/Glycerol-Kinase-Antibody-Western-Blot-NBP3-09340-img0001.jpg "Western Blot: Glycerol Kinase Antibody [NBP3-09340] - Western blot analysis of Glycerol Kinase in Mouse Stomach lysates. Antibody dilution at 1.0ug/ml")
![Western Blot: Glycerol kinase Overexpression Lysate (Adult Normal) [NBP2-11150] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glycerol kinase.](http://images.novusbio.com/fullsize/Glycerol-kinase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11150-img0001.jpg "Western Blot: Glycerol kinase Overexpression Lysate (Adult Normal) [NBP2-11150] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glycerol kinase.")
![Western Blot: Glycerol kinase Overexpression Lysate (Adult Normal) [NBL1-11101] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glycerol kinase.](http://images.novusbio.com/fullsize/Glycerol-kinase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11101-img0002.jpg "Western Blot: Glycerol kinase Overexpression Lysate (Adult Normal) [NBL1-11101] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glycerol kinase.")