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![Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-31944] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31944-img0006.jpg "Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-31944] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ferritin Heavy Chain protein stained by Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Ferritin Antibody [NBP1-31944] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Western-Blot-NBP1-31944-img0007.jpg)
![Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Western-Blot-NBP1-31944-img0009.jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Rat liver. Ferritin Heavy Chain antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Immunohistochemistry-Paraffin-NBP1-31944-img0004.jpg)
![Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-western-blot-20220248243..jpg)
![Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-western-blot-202202482557..jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Ferritin Heavy Chain stained by Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-91020242011458.jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-31944] - Ferritin Heavy Chain antibody [N1C3] detects Ferritin Heavy Chain protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Ferritin Heavy Chain stained by Ferritin Heavy Chain antibody [N1C3] (NBP1-31944) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-91020241957213.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Ferritin Heavy Chain Antibody [NBP1-31944] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [N1C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31944_rabbit-polyclonal-ferritin-heavy-chain-antibody-1610202419414513.jpg)
![Western Blot: Ferritin Heavy Chain Antibody [NBP1-06985] - Staining of Human Placenta lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-Western-Blot-NBP1-06985-img0001.jpg "Western Blot: Ferritin Heavy Chain Antibody [NBP1-06985] - Staining of Human Placenta lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody [NBP1-06985] - Human ARPE-19 cells. Antibody at 1:100 overnight. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06985-img0003.jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody [NBP1-06985] - Staining of paraffin embedded Human Liver. Antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-Immunohistochemistry-Paraffin-NBP1-06985-img0002.jpg)
![Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.](http://images.novusbio.com/fullsize/Ferritin-Antibody-Western-Blot-NB600-920-img0004.jpg "Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.")
![Western Blot: Ferritin Antibody (962610) [MAB9354] - Analysis of human liver, spleen tissue lysates with 5 ug/ml of hFerritin Monoclonal Antibody followed by HAF018. A specific band was detected for hFerritin at approximately20 kDA (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-962610-Western-Blot-MAB9354-img0001.jpg "Western Blot: Ferritin Antibody (962610) [MAB9354] - Analysis of human liver, spleen tissue lysates with 5 ug/ml of hFerritin Monoclonal Antibody followed by HAF018. A specific band was detected for hFerritin at approximately20 kDA (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry/Immunofluorescence: Ferritin Antibody (962610) [MAB9354] - Ferritin was detected in iBJ6 hepatocyte cells using Ferritin Monoclonal Antibody at 1 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear region.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-962610-Immunocytochemistry-Immunofluorescence-MAB9354-img0003.jpg)
![Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-26220241447157.jpg "Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-27220248573313.jpg)
![Western Blot: Ferritin Antibody (12H1) [NBP3-26568] - Positive Western Blot detected in: U251 whole cell lysate, JK whole cell lysate. All lanes: Ferritin Antibody at 1: 500 Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-282202411485815.jpg)
![Flow Cytometry: Ferritin Antibody (12H1) [NBP3-26568] - Overlay Peak curve showing 293 cells stained with Ferritin Antibody (12H1) (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26568_mouse-ferritin-mab-12h1-282202414312738.jpg)
![Western Blot: Ferritin Heavy Chain Antibody (1Q10I7) [NBP3-15766] - Western blot analysis of extracts of various cell lines, using Ferritin Heavy Chain antibody (NBP3-15766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-1Q10I7-Western-Blot-NBP3-15766-img0003.jpg "Western Blot: Ferritin Heavy Chain Antibody (1Q10I7) [NBP3-15766] - Western blot analysis of extracts of various cell lines, using Ferritin Heavy Chain antibody (NBP3-15766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody (1Q10I7) [NBP3-15766] - Immunohistochemistry of paraffin-embedded human lung using Ferritin Heavy Chain Rabbit mAb (NBP3-15766) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-1Q10I7-Immunohistochemistry-Paraffin-NBP3-15766-img0002.jpg)
![Western Blot: Ferritin Heavy Chain Antibody (1Q10I7) [NBP3-15766] - Western blot analysis of extracts of U-251MG cells, using Ferritin Heavy Chain antibody (NBP3-15766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-1Q10I7-Western-Blot-NBP3-15766-img0004.jpg)
![Immunohistochemistry-Paraffin: Ferritin Heavy Chain Antibody (1Q10I7) [NBP3-15766] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Ferritin Heavy Chain Rabbit mAb (NBP3-15766) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ferritin-Heavy-Chain-Antibody-1Q10I7-Immunohistochemistry-Paraffin-NBP3-15766-img0001.jpg)
![ELISA: Human Ferritin ELISA Kit (Colorimetric) [NBP2-60467] - Representative standard curve.](http://images.novusbio.com/fullsize/Human-Ferritin-ELISA-Kit-Colorimetric-ELISA-NBP2-60467-img0001.jpg "ELISA: Human Ferritin ELISA Kit (Colorimetric) [NBP2-60467] - Representative standard curve.")
![Western Blot: Ferritin Heavy Chain Antibody (S03-3D9) [NBP3-15028] - Western blot detection of Ferritin Heavy Chain in K562, CHO-K1, Hela cell lysates using Ferritin Heavy Chain Rabbit mAb (1:1000 diluted). Predicted band size: 21KDa. Observed band size: 21KDa.](http://images.novusbio.com/fullsize/Ferritin-Antibody-S03-3D9-Western-Blot-NBP3-15028-img0002.jpg "Western Blot: Ferritin Heavy Chain Antibody (S03-3D9) [NBP3-15028] - Western blot detection of Ferritin Heavy Chain in K562, CHO-K1, Hela cell lysates using Ferritin Heavy Chain Rabbit mAb (1:1000 diluted). Predicted band size: 21KDa. Observed band size: 21KDa.")
![Immunocytochemistry/Immunofluorescence: Ferritin Heavy Chain Antibody (S03-3D9) [NBP3-15028] - Immunofluorescence of Ferritin Heavy Chain (green) in Hela cells using Ferritin Rabbit mAb at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/Ferritin-Antibody-S03-3D9-Immunocytochemistry-Immunofluorescence-NBP3-15028-img0003.jpg)
![Western Blot: Ferritin Heavy Chain Antibody (S03-3D9) [NBP3-15028] - Western blot detection of Ferritin in Raw264. 7 cell lysates using Ferritin Heavy Chain Rabbit mAb (1:1000 diluted). Predicted band size: 21KDa. Observed band size: 21KDa.](http://images.novusbio.com/fullsize/Ferritin-Antibody-S03-3D9-Western-Blot-NBP3-15028-img0001.jpg)
![Western Blot: Ferritin Antibody (39G3) [NBP3-26567] - Positive Western Blot detected in: Mouse Liver tissue lysate. All lanes: Ferritin Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 21 kDa Observed band size: 21 kDa](http://images.novusbio.com/fullsize/nbp3-26567_rabbit-ferritin-mab-39g3-28220241135167.jpg "Western Blot: Ferritin Antibody (39G3) [NBP3-26567] - Positive Western Blot detected in: Mouse Liver tissue lysate. All lanes: Ferritin Antibody at 1: 500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 21 kDa Observed band size: 21 kDa")
![Immunocytochemistry/Immunofluorescence: Ferritin Antibody (39G3) [NBP3-26567] - Staining of HepG2 with Ferritin Antibody (39G3) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 497-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26567_rabbit-ferritin-mab-39g3-282202412475126.jpg)
![Flow Cytometry: Ferritin Antibody (39G3) [NBP3-26567] - Overlay Peak curve showing 293 cells stained with Ferritin Antibody (39G3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26567_rabbit-ferritin-mab-39g3-282202414312769.jpg)
![Western Blot: Ferritin Antibody (FTH/2082) - Azide and BSA Free [NBP3-08613] - Western Blot Analysis of HeLa cell lysate using Ferritin Mouse Monoclonal Antibody (FTH/2082).](http://images.novusbio.com/fullsize/Ferritin-Antibody-FTH-2082-Azide-and-BSA-Free-Western-Blot-NBP3-08613-img0002.jpg "Western Blot: Ferritin Antibody (FTH/2082) - Azide and BSA Free [NBP3-08613] - Western Blot Analysis of HeLa cell lysate using Ferritin Mouse Monoclonal Antibody (FTH/2082).")
![Immunohistochemistry-Paraffin: Ferritin Antibody (FTH/2082) - Azide and BSA Free [NBP3-08613] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin Mouse Monoclonal Antibody (FTH/2082).](http://images.novusbio.com/fullsize/Ferritin-Antibody-FTH-2082-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08613-img0001.jpg)
