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![Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP expression in transfected 293T cell line by FAP monoclonal antibody (M01), clone 1E5.Lane 1: FAP transfected lysate(87.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-1E5-Western-Blot-H00002191-M01-img0005.jpg "Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP expression in transfected 293T cell line by FAP monoclonal antibody (M01), clone 1E5.Lane 1: FAP transfected lysate(87.7 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - FAP monoclonal antibody (M01), clone 1E5 Analysis of FAP expression in HepG2.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-1E5-Western-Blot-H00002191-M01-img0004.jpg)
![Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Analysis of FAP transfected lysate using anti-FAP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FAP MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-1E5-Immunoprecipitation-H00002191-M01-img0002.jpg)
![ELISA: Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Fibroblast Activation Protein alpha/FAP Antibody (1E5) [H00002191-M01] - Detection limit for recombinant GST tagged FAP is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-1E5-ELISA-H00002191-M01-img0006.jpg)
![N/A Fibroblast Activation Protein alpha/FAP [Biotin]](http://images.novusbio.com/fullsize2/DATA_Fibroblast_Activation_Protein_alpha_DY3715_ELISA_1975.jpg "N/A Fibroblast Activation Protein alpha/FAP [Biotin]")
![Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Western blot using affinity purified anti-Fibroblast Activation protein alpha/Fibroblast Activation Protein alpha/FAP antibody shows detection of Fibroblast Activation Protein alpha/FAP protein in whole cell lysates from Fibroblast Activation Protein alpha/FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected Fibroblast Activation Protein alpha/FAP. Primary antibody was used at 1:1,000.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-Western-Blot-NB110-85534-img0006.jpg "Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Western blot using affinity purified anti-Fibroblast Activation protein alpha/Fibroblast Activation Protein alpha/FAP antibody shows detection of Fibroblast Activation Protein alpha/FAP protein in whole cell lysates from Fibroblast Activation Protein alpha/FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected Fibroblast Activation Protein alpha/FAP. Primary antibody was used at 1:1,000.")
![ELISA: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - ELISA results of purified Fibroblast Activation protein alpha/FAP antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-ELISA-NB110-85534-img0007.jpg)
![Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Specific co-culture system created to study human macrophage cancer cells' conduction of MMT process, and Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] (Catalog # NB110-85534AF488) was used as a myo-CAFs marker. Image from a verified customer review.](http://images.novusbio.com/fullsize/nb110-85534_rabbit-polyclonal-fibroblast-activation-protein-alpha-fap-antibody-71020241239278.png)
![Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Whole cell lysate (500 ug per IP reaction; 5% of IPloaded) from IMR-90 cells. Antibody: Rabbit anti-FAPrecombinant monoclonal antibody used for IP at 20 ul/mg lysate. For blottingimmunoprecipitated FAP, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-Immunoprecipitation-NBP3-14728-img0001.jpg "Immunoprecipitation: Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Whole cell lysate (500 ug per IP reaction; 5% of IPloaded) from IMR-90 cells. Antibody: Rabbit anti-FAPrecombinant monoclonal antibody used for IP at 20 ul/mg lysate. For blottingimmunoprecipitated FAP, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -Whole cell lysate (50 ug) from SNB-75, HeLa, IMR-90, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti FAP recombinant monoclonal antibody [BLR150J] used at 1:1000. Secondary: HRP conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/nbp3-14728_rabbit-fibroblast-activation-protein-alpha-fap-mab-western-blot-22320249348..jpg)
![Immunohistochemistry-Paraffin-Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] - Rabbit anti-FibroblastActivation Protein alpha/FAP recombinant monoclonalantibody [BLR150J]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/nbp3-14728_rabbit-fibroblast-activation-protein-alpha-fap-mab-immunohistochemistry-paraffin-22320249549..jpg)
![ICC/IF- Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14728] -Rabbit anti-FibroblastActivation Protein alpha/FAP recombinant monoclonalantibody [BLR150J]. Secondary: HRP conjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/nbp3-14728_rabbit-fibroblast-activation-protein-alpha-fap-mab-immunocytochemistry-immunofluorescence-22320249755..jpg)
![Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (JA56-11) [NBP2-66844] -Analysis of FAP on U-87 MG cell lysates with Rabbit anti-FAP antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 88 kDaObserved band size: 100 kDaExposure time: 5 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66844_rabbit-monoclonal-fibroblast-activation-protein-alpha-fap-antibody-ja56-11-western-blot-127202491739..jpg "Western Blot: Fibroblast Activation Protein alpha/FAP Antibody (JA56-11) [NBP2-66844] -Analysis of FAP on U-87 MG cell lysates with Rabbit anti-FAP antibody at 1/1,000 dilution.Lysates/proteins at 20 ug/Lane.Predicted band size: 88 kDaObserved band size: 100 kDaExposure time: 5 minutes; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Fibroblast Activation Protein alpha/FAP Antibody (JA56-11) [NBP2-66844] - Analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-FAP antibody at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-66844_rabbit-monoclonal-fibroblast-activation-protein-alpha-fap-antibody-ja56-11-immunohistochemistry-paraffin-127202491856..jpg)
![Immunohistochemistry-Paraffin: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4853) [NBP3-13884] - Formalin-fixed, paraffin-embedded human liver stained with Fibroblast Activation Protein alpha/FAP antibody (FAP/4853).](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-FAP-4853-Immunohistochemistry-Paraffin-NBP3-13884-img0002.jpg "Immunohistochemistry-Paraffin: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4853) [NBP3-13884] - Formalin-fixed, paraffin-embedded human liver stained with Fibroblast Activation Protein alpha/FAP antibody (FAP/4853).")
![Immunohistochemistry-Paraffin: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4853) [NBP3-13884] - Formalin-fixed, paraffin-embedded human placenta stained with Fibroblast Activation Protein alpha/FAP antibody (FAP/4853). Inset: PBS instead of primary antibody, secondary only negative control.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-FAP-4853-Immunohistochemistry-Paraffin-NBP3-13884-img0001.jpg)
![Protein Array: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4853) [NBP3-13884] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Fibroblast Activation Protein alpha/FAP Antibody (FAP/4853).](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-FAP-4853-Protein-Array-NBP3-13884-img0004.jpg)
![Immunohistochemistry-Paraffin: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851) [NBP3-13883] - Formalin-fixed, paraffin-embedded human placenta stained with Fibroblast Activation Protein alpha/FAP antibody (FAP/4851). Inset: PBS instead of primary antibody, secondary only negative control.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-FAP-4851-Immunohistochemistry-Paraffin-NBP3-13883-img0001.jpg "Immunohistochemistry-Paraffin: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851) [NBP3-13883] - Formalin-fixed, paraffin-embedded human placenta stained with Fibroblast Activation Protein alpha/FAP antibody (FAP/4851). Inset: PBS instead of primary antibody, secondary only negative control.")
![Protein Array: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851) [NBP3-13883] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Fibroblast Activation Protein alpha/FAP Antibody (FAP/4851).](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-FAP-4851-Protein-Array-NBP3-13883-img0003.jpg)
![Immunohistochemistry-Paraffin: Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14320] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin, hair follicle, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Fibroblast Activation Protein alpha-FAP Antibody-Immunohistochemistry-Paraffin-NBP3-14320-img0001.jpg "Immunohistochemistry-Paraffin: Fibroblast Activation Protein alpha/FAP Antibody [NBP3-14320] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin, hair follicle, after heat-induced antigen retrieval.")