Hu,
Mu,
Rt,
Po,
Av,
Bv,
Ch,
Dr,
Gp,
Rb,
Sh,
Xp,
Ze,
CaSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ch, Dr, Gp, Rb, Sh, Xp, Ze, Ca
WB, Simple Western, Flow, ICC/IF, IHC, IP
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NB100-1965-img0011.jpg "Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.")
![Western Blot: Calnexin Antibody [NB100-1965] - Subcellular localization of TM6SF2.Immunoaffinity isolation of ER and Golgi complex from mouse liver. ER and Golgi fractions were prepared from mouse liver microsomes by immunoaffinity chromatography as described under Experimental Procedures. Microsome membranes were dissolved in RIPA buffer, and equal volumes were separated on 10% SDS-PAGE and immunoblotting as described under Experimental Procedures. BiP, binding immunoglobulin protein; Gos28, Golgi SNAP receptor complex member 1; *, nonspecific band. Image collected and cropped by Citeab from the following publication (Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NB100-1965-img0015.jpg)
![Immunohistochemistry: Calnexin Antibody [NB100-1965] - Calnexin immunostaining in the anterior (AI) and mid (MI) intestine of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5-T2 (D.I.O. zebrafish followed by 3,5-T2), D.I.O. with 3,5-T2 (D.I.O. zebrafish treated with 3,5-T2). (A) AI of ctrl zebrafish (B) AI of D.I.O. (C) AI of D.I.O. flw 3,5-T2. (D) AI of D.I.O. with 3,5-T2. The arrows indicate calnexin immunoexpression in the enteroendocrine and goblet cells. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2076-2615/10/7/1131/htm#) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-NB100-1965-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1965) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1965-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1965) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1965-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Calnexin Antibody (NB100-1965) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1965-img0020.jpg)
![Western Blot: Calnexin Antibody - BSA Free [NB100-1965] - Whole cell lysate or exosome from MDA-MD-231 cells was loaded with 20 ug/lane. 10% SDS-PAGE. Calnexin Antibody (NB100-1965) was used for primary antibody: 1:3000, 4C, overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/Calnexin-Antibody---BSA-Free-Western-Blot-NB100-1965-img0024.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on rat FR cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1965-img0023.jpg)
![Western Blot: Calnexin Antibody [NB100-1965] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NB100-1965-img0008.jpg)
![Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-NB100-1965-img0010.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on Daudi cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1965-img0021.jpg)
![Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on NIH3T3 cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Flow-Cytometry-NB100-1965-img0022.jpg)
![Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Simple-Western-NB100-1965-img0014.jpg)
![Western Blot: Calnexin Antibody - BSA Free [NB100-1965] - Hepatic apoB levels (A), liver function tests (B), & relative hepatic levels of selected transcripts encoding proteins involved in activation of fibrosis & ER stress (C) in male WT & Tm6sf2−/− mice described in Fig. 3A.A, immunoblotting analysis was performed on liver protein (50 μg) using a rabbit anti-mouse apoB polyclonal antibody (1:1,000; Abcam) & ECL (SuperSignal West Pico Kit, Thermo Scientific). The ECL signal was visualized using a LI-COR imager (Odyssey Fc imager) & analyzed using LI-COR Image Studio software. B, plasma levels of aspartate aminotransferase (AST) & alanine aminotransferase (ALT) in chow-fed male mice. C, RNA levels were detected using quantitative real-time PCR (qRT-PCR), normalized to levels of 36B4 & expressed relative to the levels in the WT animals (n = 5). COL1A1, collagen alpha-1(I) chain; ACTA2, actin, aortic smooth muscle; XBP1s/u, X-box-binding protein 1 spliced/unspliced; ATF4, activating transcription factor 4; EDEM, ER degradation enhancer, mannosidase alpha -like 1; CHOP(DDIT3), C/EBP-homologous protein. Values are means ± S.E. (error bars). *, p < 0.05. AU, arbitrary units. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27013658), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1965_rabbit-polyclonal-calnexin-antibody-3102024165733.jpg)
![Immunohistochemistry-Paraffin: TMEM106C Antibody [NBP2-13442] - Staining in human parathyroid gland and skeletal muscle tissues using anti-TMEM106C antibody. Corresponding TMEM106C RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TMEM106C-Antibody-Immunohistochemistry-Paraffin-NBP2-13442-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TMEM106C Antibody [NBP2-13442] - Staining in human parathyroid gland and skeletal muscle tissues using anti-TMEM106C antibody. Corresponding TMEM106C RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TMEM106C Antibody [NBP2-13442] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/TMEM106C-Antibody-Immunohistochemistry-Paraffin-NBP2-13442-img0003.jpg)
![Immunohistochemistry-Paraffin: TMEM106C Antibody [NBP2-13442] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/TMEM106C-Antibody-Immunohistochemistry-Paraffin-NBP2-13442-img0002.jpg)
![Western Blot: SERP1 Antibody [NBP2-54686] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/SERP1-Antibody-Western-Blot-NBP2-54686-img0003.jpg "Western Blot: SERP1 Antibody [NBP2-54686] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)")
![Immunocytochemistry/Immunofluorescence: SERP1 Antibody [NBP2-54686] - Staining of human cell line A-431 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/SERP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54686-img0005.jpg)
![Immunohistochemistry-Paraffin: SERP1 Antibody [NBP2-54686] - Staining of human pancreas shows strong cytoplasmic positivity in Islets of Langerhans.](http://images.novusbio.com/fullsize/SERP1-Antibody-Immunohistochemistry-Paraffin-NBP2-54686-img0001.jpg)
![Western Blot: SERP1 Antibody [NBP2-54686] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251 MG Lane 4: Human plasma Lane 5: Human Liver tissue](http://images.novusbio.com/fullsize/SERP1-Antibody-Western-Blot-NBP2-54686-img0002.jpg)
![Western Blot: TMEM106C Overexpression Lysate (Adult Normal) [NBP2-09695] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMEM106C.](http://images.novusbio.com/fullsize/TMEM106C-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09695-img0001.jpg "Western Blot: TMEM106C Overexpression Lysate (Adult Normal) [NBP2-09695] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMEM106C.")
![Western Blot: TMEM106C Overexpression Lysate (Adult Normal) [NBL1-16995] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMEM106C.](http://images.novusbio.com/fullsize/TMEM106C-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16995-img0002.jpg "Western Blot: TMEM106C Overexpression Lysate (Adult Normal) [NBL1-16995] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMEM106C.")
![Immunohistochemistry-Paraffin: TMEM106C Antibody [NBP2-97968] - Immunochemical staining of human TMEM106C in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/TMEM106C-Antibody-Immunohistochemistry-Paraffin-NBP2-97968-img0001.jpg "Immunohistochemistry-Paraffin: TMEM106C Antibody [NBP2-97968] - Immunochemical staining of human TMEM106C in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: SERP1 Antibody [NBP1-98592] - Titration: 1.0 ug/ml Positive Control: MCF7 Whole Cell.](http://images.novusbio.com/fullsize/SERP1-Antibody-Western-Blot-NBP1-98592-img0002.jpg "Western Blot: SERP1 Antibody [NBP1-98592] - Titration: 1.0 ug/ml Positive Control: MCF7 Whole Cell.")
![ELISA: Rat SERP1 ELISA Kit (Colorimetric) [NBP2-80304] - Samples were spiked with high concentrations of Rat SERP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80304_rat-serp1-elisa-kit-colorimetric-132202416264329.jpg "ELISA: Rat SERP1 ELISA Kit (Colorimetric) [NBP2-80304] - Samples were spiked with high concentrations of Rat SERP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat SERP1 ELISA Kit (Colorimetric) [NBP2-80304] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80304_rat-serp1-elisa-kit-colorimetric-4320249141640.jpg)
![Western Blot: SERP1 Overexpression Lysate (Adult Normal) [NBL1-15831] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SERP1.](http://images.novusbio.com/fullsize/SERP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15831-img0002.jpg "Western Blot: SERP1 Overexpression Lysate (Adult Normal) [NBL1-15831] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SERP1.")