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![Western Blot: CYLD Antibody [NB100-56461] - Analysis of CYLD in HeLa cell lysate using this antibody.](http://images.novusbio.com/fullsize/CYLD-Antibody-Western-Blot-NB100-56461-img0006.jpg "Western Blot: CYLD Antibody [NB100-56461] - Analysis of CYLD in HeLa cell lysate using this antibody.")
![Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56461-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-56461] - Staining of HeLa cell using this antibody at 5 ug/ml. ICC staining of HeLa cell using control (rabbit Ig) at 5 ug/ml.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56461-img0007.jpg)
![Simple Western: CYLD Antibody [NB100-56461] - Simple Western lane view shows a specific band for CYLD in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CYLD-Antibody-Simple-Western-NB100-56461-img0008.jpg)
![Western Blot: CYLD Antibody [NB100-78600] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CYLD-Antibody-Western-Blot-NB100-78600-img0018.jpg "Western Blot: CYLD Antibody [NB100-78600] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CYLD Antibody [NB100-78600] - Analysis of paraformaldehyde-fixed A431, antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunocytochemistry-Immunofluorescence-NB100-78600-img0015.jpg)
![Immunohistochemistry-Paraffin: CYLD Antibody [NB100-78600] - Paraffin-embedded gastric CA, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunohistochemistry-Paraffin-NB100-78600-img0016.jpg)
![Western Blot: CYLD Antibody [NB100-78600] - A. C8D30 whole cell lysate/extract. B. NIH-3T3 whole cell lysate/extract. C. BCL-1 whole cell lysate/extract. D. Raw264.7 whole cell lysate/extract. E. C2C12 whole cell lysate/extract.](http://images.novusbio.com/fullsize/CYLD-Antibody-Western-Blot-NB100-78600-img0011.jpg)
![Western Blot: CYLD Antibody [NB100-78600] - A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGECYLD antibody dilution: 1:500.](http://images.novusbio.com/fullsize/CYLD-Antibody-Western-Blot-NB100-78600-img0014.jpg)
![Western Blot: CYLD Antibody [NB100-78600] - CYLD antibody (1:1000) detecs a specific band at ~100kDa in whole cell lysate from cultured mouse osteoclasts. Data provided by Tamer Hadi, Virginia Commonwealth University.](http://images.novusbio.com/fullsize/CYLD-Antibody-Western-Blot-NB100-78600-img0017.jpg)
![Western Blot: CYLD Antibody [NBP2-92436] - Western blot analysis of extracts of various cell lines, using CYLD antibody (NBP2-92436) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CYLD Antibody-Western Blot-NBP2-92436-img0006.jpg "Western Blot: CYLD Antibody [NBP2-92436] - Western blot analysis of extracts of various cell lines, using CYLD antibody (NBP2-92436) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: CYLD Antibody [NBP2-92436] - Immunohistochemistry of paraffin-embedded mouse spleen using CYLD Rabbit pAb (NBP2-92436) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunohistochemistry-Paraffin-NBP2-92436-img0005.jpg)
![Immunohistochemistry-Paraffin: CYLD Antibody [NBP2-92436] - Immunohistochemistry of paraffin-embedded rat brain using CYLD Rabbit pAb (NBP2-92436) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunohistochemistry-Paraffin-NBP2-92436-img0001.jpg)
![Immunohistochemistry-Paraffin: CYLD Antibody [NBP2-92436] - Immunohistochemistry of paraffin-embedded rat spleen using CYLD Rabbit pAb (NBP2-92436) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunohistochemistry-Paraffin-NBP2-92436-img0002.jpg)
![Immunohistochemistry-Paraffin: CYLD Antibody [NBP2-92436] - Immunohistochemistry of paraffin-embedded human lung cancer using CYLD Rabbit pAb (NBP2-92436) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunohistochemistry-Paraffin-NBP2-92436-img0003.jpg)
![Immunohistochemistry-Paraffin: CYLD Antibody [NBP2-92436] - Immunohistochemistry of paraffin-embedded human esophageal cancer using CYLD Rabbit pAb (NBP2-92436) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunohistochemistry-Paraffin-NBP2-92436-img0004.jpg)
![Western Blot: CYLD Antibody (HL1260) - Azide and BSA Free [NBP3-25425] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1260] (NBP3-25425) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25425_rabbit-cyld-mab-hl1260-azide-and-bsa-free-2120249351332.jpg "Western Blot: CYLD Antibody (HL1260) - Azide and BSA Free [NBP3-25425] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1260] (NBP3-25425) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CYLD Antibody (HL1260) - Azide and BSA Free [NBP3-25425] - CYLD antibody [HL1260] detects CYLD protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CYLD stained by CYLD antibody [HL1260] (NBP3-25425) diluted at 1:100. Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25425_rabbit-cyld-mab-hl1260-azide-and-bsa-free-212024939132.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CYLD Antibody (HL1260) - Azide and BSA Free [NBP3-25425] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CYLD antibody [HL1260] (NBP3-25425) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25425_rabbit-cyld-mab-hl1260-azide-and-bsa-free-2120249393584.jpg)
![Immunocytochemistry/Immunofluorescence: CYLD Antibody (HL1260) - Azide and BSA Free [NBP3-25425] - CYLD antibody [HL1260] detects CYLD protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYLD stained by CYLD antibody [HL1260] (NBP3-25425) diluted at 1:685. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25425_rabbit-cyld-mab-hl1260-azide-and-bsa-free-212024937151.jpg)
![Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10Lane 2: RT-4Lane 3: U-251MGLane 4: Human PlasmaLane 5: LiverLane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-24772_rabbit-cyld-pab-201220231521515.jpg)
![Immunocytochemistry/Immunofluorescence: CYLD Antibody [NBP2-33589] - Staining of human cell line A-431 shows localization to microtubule organizing center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33589-img0003.jpg "Immunocytochemistry/Immunofluorescence: CYLD Antibody [NBP2-33589] - Staining of human cell line A-431 shows localization to microtubule organizing center. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: CYLD Antibody [NBP2-33589] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/CYLD-Antibody-Immunohistochemistry-Paraffin-NBP2-33589-img0002.jpg)
![Western Blot: CYLD Overexpression Lysate (Adult Normal) [NBP2-08356] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYLD.](http://images.novusbio.com/fullsize/CYLD-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08356-img0002.jpg "Western Blot: CYLD Overexpression Lysate (Adult Normal) [NBP2-08356] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYLD.")
![Western Blot: CYLD Antibody (HL1261) - Azide and BSA Free [NBP3-25426] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1261] (NBP3-25426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25426_rabbit-cyld-mab-hl1261-azide-and-bsa-free-2120249413370.jpg "Western Blot: CYLD Antibody (HL1261) - Azide and BSA Free [NBP3-25426] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1261] (NBP3-25426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CYLD Overexpression Lysate (Adult Normal) [NBP2-08360] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYLD.](http://images.novusbio.com/fullsize/CYLD-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08360-img0001.jpg "Western Blot: CYLD Overexpression Lysate (Adult Normal) [NBP2-08360] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYLD.")
![ELISA: Human CYLD ELISA Kit (Colorimetric) [NBP3-27310] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27310_human-cyld-elisa-kit-colorimetric-114202410253623.png "ELISA: Human CYLD ELISA Kit (Colorimetric) [NBP3-27310] - Standard Curve Reference")
![ELISA: Human CYLD - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31314] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31314_human-cyld-ready-to-use-elisa-kit-colorimetric-206202415322790.png "ELISA: Human CYLD - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31314] - Standard Curve Reference")