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![Western Blot: CBP/KAT3A Antibody [NB100-381] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-381 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Western-Blot-NB100-381-img0032.jpg "Western Blot: CBP/KAT3A Antibody [NB100-381] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-381 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: CBP/KAT3A Antibody [NB100-381] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody used for IP at 6 ug/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody NB100-382. For blotting immunoprecipitated CBP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunoprecipitation-NB100-381-img0031.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249432892.jpg "Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249422641.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - Wild-type (WT) and CREBBP knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-212024942543.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-212024939130.jpg)
![Western Blot: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [HL2197] (NBP3-25399) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249393587.jpg)
![Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody (HL2197) - Azide and BSA Free [NBP3-25399] - CBP antibody [HL2197] detects CBP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CBP stained by CBP antibody [HL2197] (NBP3-25399) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25399_rabbit-cbp-kat3a-mab-hl2197-azide-and-bsa-free-2120249432876.jpg)
![Western Blot: CBP/KAT3A Antibody [NB100-382] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Western-Blot-NB100-382-img0008.jpg "Western Blot: CBP/KAT3A Antibody [NB100-382] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry: CBP/KAT3A Antibody [NB100-382] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBP used at a dilution of 1:500 (2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-NB100-382-img0007.jpg)
![Immunohistochemistry: CBP/KAT3A Antibody [NB100-382] - FFPE section of human prostate adenocarcinoma. Antibody used at a dilution of 1:250. Detection: DAB.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-NB100-382-img0003.jpg)
![Immunoprecipitation: CBP/KAT3A Antibody [NB100-382] - Samples: Nuclear (NE) or cytoplasmic (S100) extract (10 mg) from HeLa cells. Antibody: Affinity purified rabbit anti-CBP used at 20 ug/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is CBP.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunoprecipitation-NB100-382-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CBP/KAT3A Antibody [NB100-382] - Detection of human CBP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-382 used for IP at 6 ug/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody NB100-381. For blotting immunoprecipitated CBP, NB100-382 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunoprecipitation-NB100-382-img0009.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Analysis in human endometrium and liver tissues. Corresponding CREBBP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Analysis in human endometrium and liver tissues. Corresponding CREBBP RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody [NBP2-38774] - Staining of human cell line MCF7 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38774-img0001.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human liver shows very weak nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0002.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human endometrium shows strong nuclear positivity in glandular cells and stromal cells.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0003.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0004.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP2-38774] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP2-38774-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody (2H5) [H00001387-M01] - Analysis of monoclonal antibody to CREBBP on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-2H5-Immunocytochemistry-Immunofluorescence-H00001387-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody (2H5) [H00001387-M01] - Analysis of monoclonal antibody to CREBBP on HeLa cell . Antibody concentration 10 ug/ml.")
![Proximity Ligation Assay: CBP/KAT3A Antibody (2H5) [H00001387-M01] - Protein-protein interactions between FGFR1 and CREBBP HeLa cells were stained with anti-FGFR1 (H00002260-D01P) rabbit purified polyclonal 1:1200 and anti-CREBBP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-2H5-Proximity-Ligation-Assay-H00001387-M01-img0003.jpg)
![Sandwich ELISA: CBP/KAT3A Antibody (2H5) [H00001387-M01] - Detection limit for recombinant GST tagged CREBBP is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-2H5-Sandwich-ELISA-H00001387-M01-img0006.jpg)
![Western Blot: CBP/KAT3A Antibody [NBP1-52906] - Jurkat cell lysate, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Western-Blot-NBP1-52906-img0003.jpg "Western Blot: CBP/KAT3A Antibody [NBP1-52906] - Jurkat cell lysate, concentration 1 ug/ml.")
![Immunohistochemistry: CBP/KAT3A Antibody [NBP1-52906] - Analysis of human breast after heat-induced Antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-NBP1-52906-img0004.jpg)
![Immunohistochemistry-Paraffin: CBP/KAT3A Antibody [NBP1-52906] - Human Colon Tissue, antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunohistochemistry-Paraffin-NBP1-52906-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody [NBP2-98753] - Immunofluorescence staining of CBP/KAT3A in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum. Then incubated with rabbit anti-human CBP/KAT3A polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human CBP/KAT3A polyclonal antibody and antigen (Figure B) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98753-img0001.jpg "Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody [NBP2-98753] - Immunofluorescence staining of CBP/KAT3A in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum. Then incubated with rabbit anti-human CBP/KAT3A polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human CBP/KAT3A polyclonal antibody and antigen (Figure B) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.")
![Sandwich ELISA: CBP/KAT3A Antibody (2B6) [H00001387-M02] - Detection limit for recombinant GST tagged CREBBP is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CBP-KAT3A-Antibody-2B6-Sandwich-ELISA-H00001387-M02-img0001.jpg "Sandwich ELISA: CBP/KAT3A Antibody (2B6) [H00001387-M02] - Detection limit for recombinant GST tagged CREBBP is 0.1 ng/ml as a capture antibody.")
![ELISA: Human CBP/KAT3A - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31301] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31301_human-cbp-kat3a-ready-to-use-elisa-kit-colorimetric-206202415301572.png "ELISA: Human CBP/KAT3A - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31301] - Standard Curve Reference")