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![Western Blot: BFAR Antibody [NB100-56094] - Analysis of BAR in cell lines and tissues. 25 ug of total protein was loaded per lane. BAR Recombinant protein (RP) was used as a positive control.](http://images.novusbio.com/fullsize/BFAR-Antibody-Western-Blot-NB100-56094-img0004.jpg "Western Blot: BFAR Antibody [NB100-56094] - Analysis of BAR in cell lines and tissues. 25 ug of total protein was loaded per lane. BAR Recombinant protein (RP) was used as a positive control.")
![Immunohistochemistry-Paraffin: BFAR Antibody [NB100-56094] - Staining of normal Human Brain. 1:2000. A) Cerebellum. B) Medulla. A1 and B1 are higher magnifications of A and B, respectively.](http://images.novusbio.com/fullsize/BFAR-Antibody-Immunohistochemistry-Paraffin-NB100-56094-img0006.jpg)
![Immunohistochemistry-Paraffin: BFAR Antibody [NB100-56094] - Staining of tumor and normal Human tissues stained for BAR expression using this antibody at 1:2000. A. Pancreatic cancer. B. Normal pancreas. C. Esophageal cancer. D. Normal esophagus. Increased expression of BAR was detected in the tumor, compared to normal tissues. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/BFAR-Antibody-Immunohistochemistry-Paraffin-NB100-56094-img0005.jpg)
![Western Blot: BFAR Antibody [NB100-56093] - Analysis of BAR in normal prostate and prostate carcinoma tissue lysates. 25 ug protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54 kDa. This is higher than the predicted molecular weight from the 450 amino acid BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.](http://images.novusbio.com/fullsize/BFAR-Antibody-Western-Blot-NB100-56093-img0004.jpg "Western Blot: BFAR Antibody [NB100-56093] - Analysis of BAR in normal prostate and prostate carcinoma tissue lysates. 25 ug protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54 kDa. This is higher than the predicted molecular weight from the 450 amino acid BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.")
![Immunohistochemistry-Paraffin: BFAR Antibody [NB100-56093] - Formalin-fixed, paraffin-embedded human prostate carcinoma tissue array stained for BAR expression using this antibody at 1:2000. Hematoxylin-eosin counterstain. Variable BAR expression is seen between patient samples.](http://images.novusbio.com/fullsize/BFAR-Antibody-Immunohistochemistry-Paraffin-NB100-56093-img0005.jpg)
![Immunohistochemistry-Paraffin: BFAR Antibody [NB100-56093] - Formalin-fixed, paraffin-embedded tissue sections stained for BAR expression using this antibody at 1:2000. A. Two cores from a human gliobastoma tissue microarray: 1 = fibrillary astrocytoma (grade I), and 2 = anaplastic glioma (grade III). B. Higher magnification from the fibrillary astrocytoma (shown in A). C. Higher magnification from the anaplastic glioma (shown in A). D. Normal human brain striatum with positive medium spiny neurons, the major neuronal cell type of the striatum. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/BFAR-Antibody-Immunohistochemistry-Paraffin-NB100-56093-img0003.jpg)
![Western Blot: BFAR Antibody [NBP2-92687] - Analysis of extracts of various cell lines, using BFAR at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/BFAR-Antibody-Western-Blot-NBP2-92687-img0003.jpg "Western Blot: BFAR Antibody [NBP2-92687] - Analysis of extracts of various cell lines, using BFAR at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: BFAR Antibody [NBP2-92687] - Immunofluorescence analysis of C6 cells using BFAR Polyclonal Antibody (NBP2-92687) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/BFAR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92687-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: BFAR Antibody [NBP2-92687] - Analysis of NIH-3T3 cells using BFAR . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/BFAR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92687-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BFAR Antibody [NBP2-92687] - Analysis of U-2 OS cells using BFAR . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/BFAR-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92687-img0002.jpg)
![Western Blot: BFAR Antibody [NBP2-86584] - WB Suggested Anti-Bfar Antibody. Titration: 1.0 ug/ml. Positive Control: Mouse Brain](http://images.novusbio.com/fullsize/BFAR-Antibody-Western-Blot-NBP2-86584-img0001.jpg "Western Blot: BFAR Antibody [NBP2-86584] - WB Suggested Anti-Bfar Antibody. Titration: 1.0 ug/ml. Positive Control: Mouse Brain")
![Western Blot: BFAR Overexpression Lysate (Adult Normal) [NBP2-04359] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BFAR.](http://images.novusbio.com/fullsize/BFAR-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04359-img0001.jpg "Western Blot: BFAR Overexpression Lysate (Adult Normal) [NBP2-04359] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BFAR.")