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![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90063] - Analysis in human testis and skeletal muscle tissues. Corresponding Ataxin-2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90063-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90063] - Analysis in human testis and skeletal muscle tissues. Corresponding Ataxin-2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90063] - Staining of human cerebellum, cerebral cortex, skeletal muscle and testis using Anti-Ataxin-2 antibody NBP1-90063 (A) shows similar protein distribution across tissues to independent antibody NBP1-90062 (B).](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90063-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP1-90063] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90063-img0009.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90063] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90063-img0028.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90063] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90063-img0023.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90063] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90063-img0025.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90063] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90063-img0027.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90064] - Staining in human testis and skeletal muscle tissues using NBP1-90064 antibody. Corresponding ATXN2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90064-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90064] - Staining in human testis and skeletal muscle tissues using NBP1-90064 antibody. Corresponding ATXN2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90064] - Staining of human cerebellum, cerebral cortex, skeletal muscle and testis using Anti-ATXN2 antibody NBP1-90064 (A) shows similar protein distribution across tissues to independent antibody NBP1-90063 (B).](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90064-img0016.jpg)
![Western Blot: Ataxin-2 Antibody [NBP1-90064] - Analysis in human cell line K562.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Western-Blot-NBP1-90064-img0014.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90064] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90064-img0021.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90064] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90064-img0017.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90064] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90064-img0018.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90064] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90064-img0019.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Analysis in human testis and skeletal muscle tissues. Corresponding Ataxin-2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Analysis in human testis and skeletal muscle tissues. Corresponding Ataxin-2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebellum, cerebral cortex, skeletal muscle and testis using Anti-Ataxin-2 antibody NBP1-90062 (A) shows similar protein distribution across tissues to independent antibody NBP1-90063 (B).](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0017.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0018.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0010.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0014.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody [NBP1-90062] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-90062-img0016.jpg)
![Immunohistochemistry-Paraffin: Ataxin-2 Antibody (HL1902) - Azide and BSA Free [NBP3-25373] - ATXN2 antibody [HL1902] detects ATXN2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ATXN2 stained by ATXN2 antibody [HL1902] (NBP3-25373) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25373_rabbit-ataxin-2-mab-hl1902-azide-and-bsa-free-2120249342784.jpg "Immunohistochemistry-Paraffin: Ataxin-2 Antibody (HL1902) - Azide and BSA Free [NBP3-25373] - ATXN2 antibody [HL1902] detects ATXN2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ATXN2 stained by ATXN2 antibody [HL1902] (NBP3-25373) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody (HL1902) - Azide and BSA Free [NBP3-25373] - ATXN2 antibody [HL1902] detects ATXN2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATXN2 stained by ATXN2 antibody [HL1902] (NBP3-25373) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25373_rabbit-ataxin-2-mab-hl1902-azide-and-bsa-free-2120249422651.jpg)
![Western Blot: Ataxin-2 Antibody (HL1902) - Azide and BSA Free [NBP3-25373] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATXN2 antibody [HL1902] (NBP3-25373) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25373_rabbit-ataxin-2-mab-hl1902-azide-and-bsa-free-2120249351391.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody (HL1902) - Azide and BSA Free [NBP3-25373] - ATXN2 antibody [HL1902] detects ATXN2 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATXN2 stained by ATXN2 antibody [HL1902] (NBP3-25373) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25373_rabbit-ataxin-2-mab-hl1902-azide-and-bsa-free-2120249371589.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunofluorescence-NBP2-76807-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).")
![Flow Cytometry: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Flow-Cytometry-NBP2-76807-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Ataxin-2 Antibody [NBP2-76807] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunofluorescence-NBP2-76807-img0002.jpg)
![SDS-Page: Recombinant Human Ataxin-2 Protein [H00006311-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006311-Q01-1.jpg "SDS-Page: Recombinant Human Ataxin-2 Protein [H00006311-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Ataxin-2 Antibody [NB100-58797] - Detection of Human and Mouse ATX2/SCA2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-ATX2/SCA2 antibody NB100-58797 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Western-Blot-NB100-58797-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ataxin-2 Antibody [NB100-58797] - Detection of Human and Mouse ATX2/SCA2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-ATX2/SCA2 antibody NB100-58797 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: Ataxin-2 Antibody [NB100-58797] - Detection of human ATX2/SCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-ATX2/SCA2 antibody NB100-58797 used for IP at 6 ug/mg lysate. ATX2/SCA2 was also immunoprecipitated by rabbit anti-ATX2/SCA2 antibody from Company B. For blotting immunoprecipitated ATX2/SCA2, NB100-58797 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Immunoprecipitation-NB100-58797-img0004.jpg)
![Knockdown Validated: Ataxin-2 Antibody (HL1903) - Azide and BSA Free [NBP3-25374] - Non-transfected (-) and transfected (+) transfected 293T (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATXN2 antibody [HL1903] (NBP3-25374) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25374_rabbit-ataxin-2-mab-hl1903-azide-and-bsa-free-2120249355558.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: Ataxin-2 Antibody (HL1903) - Azide and BSA Free [NBP3-25374] - Non-transfected (-) and transfected (+) transfected 293T (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATXN2 antibody [HL1903] (NBP3-25374) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Ataxin-2 Antibody (HL1903) - Azide and BSA Free [NBP3-25374] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ATXN2 antibody [HL1903] (NBP3-25374) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25374_rabbit-ataxin-2-mab-hl1903-azide-and-bsa-free-212024937155.jpg)
![ELISA: Rat Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69899] - Samples were spiked with high concentrations of Rat Ataxin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69899_rat-ataxin-2-elisa-kit-colorimetric-132202416243458.jpg "ELISA: Rat Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69899] - Samples were spiked with high concentrations of Rat Ataxin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69899] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69899_rat-ataxin-2-elisa-kit-colorimetric-432024903514.jpg)
![ELISA: Mouse Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69898] - Samples were spiked with high concentrations of Mouse Ataxin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69898_mouse-ataxin-2-elisa-kit-colorimetric-13220241681499.jpg "ELISA: Mouse Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69898] - Samples were spiked with high concentrations of Mouse Ataxin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69898] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69898_mouse-ataxin-2-elisa-kit-colorimetric-432024915169.jpg)
![ELISA: Human Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69897] - Samples were spiked with high concentrations of Human Ataxin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69897_human-ataxin-2-elisa-kit-colorimetric-13220241622104.jpg "ELISA: Human Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69897] - Samples were spiked with high concentrations of Human Ataxin-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Ataxin-2 ELISA Kit (Colorimetric) [NBP2-69897] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69897_human-ataxin-2-elisa-kit-colorimetric-4320249115634.jpg)
![Western Blot: Ataxin-2 Antibody [NBP2-99562] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:140 kDaObserved band size:140 kDa](http://images.novusbio.com/fullsize/Ataxin-2-Antibody-Western-Blot-NBP2-99562-img0001.jpg "Western Blot: Ataxin-2 Antibody [NBP2-99562] - Lane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:140 kDaObserved band size:140 kDa")