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![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of extracts of Mouse heart, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Western-Blot-NBP3-15744-img0004.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of extracts of Mouse heart, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Mouse testis using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Immunohistochemistry-Paraffin-NBP3-15744-img0002.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of extracts of various cell lines, using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Western-Blot-NBP3-15744-img0003.jpg)
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Rat kidney using Acetyl-CoA Carboxylase alpha/ACACA Rabbit mAb (NBP3-15744) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Immunohistochemistry-Paraffin-NBP3-15744-img0001.jpg)
![Immunoprecipitation: Acetyl-CoA Carboxylase alpha/ACACA Antibody (5J4W7) [NBP3-15744] - Analysis of 300ug extracts of HepG2 cells using 3ug Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-15744). Western blot was performed from the immunoprecipitate using Acetyl-CoA Carboxylase alpha/ACACA (NBP3-15744) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-5J4W7-Immunoprecipitation-NBP3-15744-img0005.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-38981] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-Western-Blot-NBP2-38981-img0002.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-38981] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-38981] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-Immunohistochemistry-Paraffin-NBP2-38981-img0007.jpg)
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-38981] - Staining of human breast shows moderate positivity in cytoplasm in glandular cells.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-Immunohistochemistry-Paraffin-NBP2-38981-img0004.jpg)
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-38981] - Staining of human cerebral cortex shows weak positivity in neuropil.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-Immunohistochemistry-Paraffin-NBP2-38981-img0005.jpg)
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-38981] - Staining of human lung shows strong cytoplasmic positivity in pneumocytes.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-Immunohistochemistry-Paraffin-NBP2-38981-img0006.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl-CoA Carboxylase alpha/ACACA (phospho Ser79) antibody (NBP3-13324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-[p-Ser79]-Antibody-Western-Blot-NBP3-13324-img0003.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl-CoA Carboxylase alpha/ACACA (phospho Ser79) antibody (NBP3-13324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl-CoA Carboxylase alpha/ACACA (phospho Ser79) antibody (NBP3-13324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-[p-Ser79]-Antibody-Western-Blot-NBP3-13324-img0001.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl-CoA Carboxylase alpha/ACACA (phospho Ser79) antibody (NBP3-13324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-[p-Ser79]-Antibody-Western-Blot-NBP3-13324-img0002.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody [NBP3-13324] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-13324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13324_rabbit-acetyl-coa-carboxylase-alpha-acaca-p-ser-79-pab-910202419593567.jpg)
![Immunocytochemistry/Immunofluorescence: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-55439] - Staining of human cell line U-251 MG shows localization to nucleoli fibrillar center, cytosol & actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55439-img0001.jpg "Immunocytochemistry/Immunofluorescence: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-55439] - Staining of human cell line U-251 MG shows localization to nucleoli fibrillar center, cytosol & actin filaments. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-55439] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/antibody/nbp2-55439_rabbit-polyclonal-acetyl-coa-carboxylase-alpha-acaca-antibody-western-blot-28102024123133..png)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (RM232) [NBP2-61471] - Western Blot of A431 cell lysates using NBP2-61471 at a 1:1000 dilution, showed a band of Acetyl CoA Carboxylase 1 (~260 kDa) expressed in A431 cells.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-RM232-Western-Blot-NBP2-61471-img0003.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA Antibody (RM232) [NBP2-61471] - Western Blot of A431 cell lysates using NBP2-61471 at a 1:1000 dilution, showed a band of Acetyl CoA Carboxylase 1 (~260 kDa) expressed in A431 cells.")
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody (RM232) [NBP2-61471] - Immunohistochemical staining of formalin fixed and paraffin embedded Human heart tissue sections using NBP2-61471 at a 1:300 dilution.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-Antibody-RM232-Immunohistochemistry-Paraffin-NBP2-61471-img0001.jpg)
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (RM270) [NBP2-61473] - Western Blot of A431 cell lysates, nontreated (-) or treated (+) with Lambda Protein Phosphatase (lPP), using NBP2-61473 at a 1:1000 dilution.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-p-Ser79-Antibody-RM270-Western-Blot-NBP2-61473-img0003.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (RM270) [NBP2-61473] - Western Blot of A431 cell lysates, nontreated (-) or treated (+) with Lambda Protein Phosphatase (lPP), using NBP2-61473 at a 1:1000 dilution.")
![Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA [p Ser79] Antibody (RM270) [NBP2-61473] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61473 at a 1:5000 dilution](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-p-Ser79-Antibody-RM270-Immunohistochemistry-Paraffin-NBP2-61473-img0002.jpg)
![Immunohistochemistry: Acetyl-CoA Carboxylase Antibody (SR1412) [NBP3-22652] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-22652.](http://images.novusbio.com/fullsize/nbp3-22652_rabbit-acetyl-coa-carboxylase-mab-sr1412-5720231754835.jpg "Immunohistochemistry: Acetyl-CoA Carboxylase Antibody (SR1412) [NBP3-22652] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-22652.")
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [ac Ser79] Antibody [NBP3-05407] - Western blot analysis of extracts of 293T cells, using Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-05407) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Acetyl-CoA-Carboxylase-alpha-ACACA-[ac-Ser79]-Antibody-Western-Blot-NBP3-05407-img0001.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [ac Ser79] Antibody [NBP3-05407] - Western blot analysis of extracts of 293T cells, using Acetyl-CoA Carboxylase alpha/ACACA antibody (NBP3-05407) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser 79] Antibody (SR1413) [NBP3-21598] - Western blot analysis using NBP3-21598 in (1) A431 cell lysate; (2) A431 cell treated with lambda phosphatase lysate.](http://images.novusbio.com/fullsize/nbp3-21598_rabbit-acaca-p-ser-79-mab-sr1413-57202317161837.jpg "Western Blot: Acetyl-CoA Carboxylase alpha/ACACA [p Ser 79] Antibody (SR1413) [NBP3-21598] - Western blot analysis using NBP3-21598 in (1) A431 cell lysate; (2) A431 cell treated with lambda phosphatase lysate.")
![SDS-Page: Recombinant Human Acetyl-CoA Carboxylase alpha/ACACA Protein [H00000031-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00000031-Q01-1.jpg "SDS-Page: Recombinant Human Acetyl-CoA Carboxylase alpha/ACACA Protein [H00000031-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")