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![Western Blot: ZHX2 Antibody [NBP1-82956] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-651](http://images.novusbio.com/fullsize/ZHX2-Antibody-Western-Blot-NBP1-82956-img0005.jpg "Western Blot: ZHX2 Antibody [NBP1-82956] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-651")
![Immunocytochemistry/Immunofluorescence: ZHX2 Antibody [NBP1-82956] - Staining of human cell line REH shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82956-img0007.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82956-img0011.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82956-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human cerebral cortex, kidney, liver and lymph node using Anti-ZHX2 antibody NBP1-82956 (A) shows similar protein distribution across tissues to independent antibody NBP1-82955 (B).](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82956-img0008.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human lymph node.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82956-img0009.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human kidney.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82956-img0010.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human liver.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82956-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: ZHX2 Antibody [NBP1-82955] - Staining of human cell line REH shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82955-img0003.jpg "Immunocytochemistry/Immunofluorescence: ZHX2 Antibody [NBP1-82955] - Staining of human cell line REH shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82955] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82955-img0007.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82955] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells and glial cells.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82955-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82955] - Staining of human cerebral cortex, kidney, liver and lymph node using Anti-ZHX2 antibody NBP1-82955 (A) shows similar protein distribution across tissues to independent antibody NBP1-82956 (B).](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82955-img0004.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82955] - Staining of human lymph node.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82955-img0005.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82955] - Staining of human kidney.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82955-img0006.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82955] - Staining of human liver.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-82955-img0008.jpg)
![Western Blot: ZHX2 Antibody [NBP1-33415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 antibody [C1C3] (NBP1-33415) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33415_rabbit-polyclonal-zhx2-antibody-910202420115015.jpg "Western Blot: ZHX2 Antibody [NBP1-33415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZHX2 antibody [C1C3] (NBP1-33415) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ZHX2 Antibody [NBP1-33415] - ZHX2 antibody detects ZHX2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZHX2 protein stained by ZHX2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33415-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ZHX2 Antibody [NBP1-33415] - Analysis of paraformaldehyde-fixed A431, using ZHX2 (NBP1-33415) antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33415-img0003.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-33415] - Paraffin-embedded SAS Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-33415-img0004.jpg)
![Western Blot: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of ZHX2 over-expressed 293 cell line, cotransfected with ZHX2 Validated Chimera RNAi ( Cat # H00022882-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZHX2 monoclonal antibody (M01), clone 5E2 (Cat # H00022882-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/ZHX2-Antibody-5E2-Western-Blot-H00022882-M01-img0009.jpg "Western Blot: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of ZHX2 over-expressed 293 cell line, cotransfected with ZHX2 Validated Chimera RNAi ( Cat # H00022882-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZHX2 monoclonal antibody (M01), clone 5E2 (Cat # H00022882-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Immunocytochemistry/Immunofluorescence: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of monoclonal antibody to ZHX2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ZHX2-Antibody-5E2-Immunocytochemistry-Immunofluorescence-H00022882-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of monoclonal antibody to ZHX2 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/ZHX2-Antibody-5E2-Immunohistochemistry-Paraffin-H00022882-M01-img0008.jpg)
![Western Blot: ZHX2 Antibody (5E2) [H00022882-M01] - Analysis of ZHX2 expression in transfected 293T cell line by ZHX2 monoclonal antibody (M01), clone 5E2.Lane 1: ZHX2 transfected lysate(92.307 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZHX2-Antibody-5E2-Western-Blot-H00022882-M01-img0010.jpg)
![Western Blot: ZHX2 Antibody (JE63-11) [NBP3-33112] - Western blot analysis of ZHX2 on different lysates with Rabbit anti-ZHX2 antibody (NBP3-33112) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: COS-1 cell lysate Lane 3: HeLa cell lysate Lane 4: C6 cell lysate Lane 5: PC-12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: AGS cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 92 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33112) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33112_rabbit-zhx2-mab-je63-11-28820241419151.jpg "Western Blot: ZHX2 Antibody (JE63-11) [NBP3-33112] - Western blot analysis of ZHX2 on different lysates with Rabbit anti-ZHX2 antibody (NBP3-33112) at 1/1,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: COS-1 cell lysate Lane 3: HeLa cell lysate Lane 4: C6 cell lysate Lane 5: PC-12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: AGS cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 92 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33112) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ZHX2 Antibody (JE63-11) [NBP3-33112] - Immunocytochemistry analysis of MCF7 (positive) and AGS (negative) labeling ZHX2 with Rabbit anti-ZHX2 antibody (NBP3-33112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ZHX2 antibody (NBP3-33112) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33112_rabbit-zhx2-mab-je63-11-29202410334077.jpg)
![Western Blot: ZHX2 Antibody (S01-9B5) [NBP3-19524] - Western blot detection of ZHX2 in Rat Brain, C6, Hela cell lysates using NBP3-19524 (1:1000 diluted). Predicted band size: 92kDa. Observed band size: 110kDa.](http://images.novusbio.com/fullsize/ZHX2-Antibody-S01-9B5-Western-Blot-NBP3-19524-img0002.jpg "Western Blot: ZHX2 Antibody (S01-9B5) [NBP3-19524] - Western blot detection of ZHX2 in Rat Brain, C6, Hela cell lysates using NBP3-19524 (1:1000 diluted). Predicted band size: 92kDa. Observed band size: 110kDa.")
![Immunocytochemistry/Immunofluorescence: ZHX2 Antibody (S01-9B5) [NBP3-19524] - Immunofluorescence of ZHX2 (green) in MCF-7 using NBP3-19524 at dilution 1/20, and DAPI (blue)](http://images.novusbio.com/fullsize/ZHX2-Antibody-S01-9B5-Immunocytochemistry-Immunofluorescence-NBP3-19524-img0001.jpg)
![Western Blot: ZHX2 Antibody [H00022882-D01P] - Analysis of ZHX2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Western-Blot-H00022882-D01P-img0003.jpg "Western Blot: ZHX2 Antibody [H00022882-D01P] - Analysis of ZHX2 expression in Hela S3 NE.")
![Western Blot: ZHX2 Antibody [H00022882-D01P] - Analysis of ZHX2 expression in transfected 293T cell line by ZHX2 polyclonal antibody.Lane 1: ZHX2 transfected lysate(92.30 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Western-Blot-H00022882-D01P-img0004.jpg)
![Western Blot: ZHX2 Antibody [H00022882-B01P] - Analysis of ZHX2 expression in human placenta.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Western-Blot-H00022882-B01P-img0003.jpg "Western Blot: ZHX2 Antibody [H00022882-B01P] - Analysis of ZHX2 expression in human placenta.")
![Western Blot: ZHX2 Antibody [H00022882-B01P] - Analysis of ZHX2 expression in transfected 293T cell line by ZHX2 polyclonal antibody. Lane 1: ZHX2 transfected lysate(92.07 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZHX2-Antibody-Western-Blot-H00022882-B01P-img0004.jpg)
![Western Blot: ZHX2 Antibody [NBP3-10465] - Western blot analysis of ZHX2 in Mouse Heart lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/ZHX2-Antibody-Western-Blot-NBP3-10465-img0001.jpg "Western Blot: ZHX2 Antibody [NBP3-10465] - Western blot analysis of ZHX2 in Mouse Heart lysates. Antibody dilution at 1.0ug/ml")
![Western Blot: ZHX2 Overexpression Lysate (Adult Normal) [NBL1-18033] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZHX2.](http://images.novusbio.com/fullsize/ZHX2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-18033-img0002.jpg "Western Blot: ZHX2 Overexpression Lysate (Adult Normal) [NBL1-18033] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZHX2.")