| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77681SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77253-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4257-SP | |||
| Carrier Free | IC4257V-100UG | |||
| Carrier Free | IC4257U-100UG | |||
| Carrier Free | IC4257T-100UG | |||
| Carrier Free | IC4257S-100UG | |||
| Carrier Free | IC4257R-100UG | |||
| Carrier Free | IC4257N-100UG | |||
| Carrier Free | IC4257G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77252-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75514-0.025mg | |||
![Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77681-img0006.jpg "Western Blot: XBP1 Antibody [NBP1-77681] - Total protein from HeLa cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-XBP1 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrow delineates XBP1s and arrowhead XBP1u.")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with XBP1 Antibody (NBP1-77681) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77681-img0010.jpg)
![Immunohistochemistry: XBP1 Antibody [NBP1-77681] - Staining of XBP1 in mouse bladder using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunohistochemistry-NBP1-77681-img0004.jpg)
![Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on NIH3T3 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/XBP1-Antibody-Flow-Cytometry-NBP1-77681-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77681] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with XBP1 Antibody (NBP1-77681) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77681-img0009.jpg)
![Flow Cytometry: XBP1 Antibody [NBP1-77681] - An intracellular stain was performed on HepG2 cells with XBP1 Antibody NBP1-77681 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/XBP1-Antibody-Flow-Cytometry-NBP1-77681-img0007.jpg)
![Western Blot: XBP1 Antibody [NBP1-77681] - Western Blot of PC-3 cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-77681_rabbit-polyclonal-xbp1-antibody-western-blot-672023151929..jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of lysates per lane. Antibodies: XBP-1 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution. (1) the absence and (2) the p](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0021.jpg "Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of lysates per lane. Antibodies: XBP-1 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution. (1) the absence and (2) the p")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77253] - analysis of 4% paraformaldehydefixed human pancreas tissue labeling XBP-1 with this antibody at20 ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77253-img0017.jpg)
![Immunohistochemistry-Paraffin: XBP1 Antibody [NBP1-77253] - analysis of paraffin-embeddedmouse spleen tissue using anti-XBP-1 antibody at 2ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was byheat mediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-77253-img0013.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Western Blot Validation in Human HepG2 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: XBP-1 NBP1-77253 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0009.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - ob/+ and ob/ob mice were injected with an adenovirus encoding human GRP78 (Ad GRP78) and a control adenovirus encoding beta-gal (Ad beta-gal). Mature nuclear form of XBP-1 expression level was reduced in ob/ob mice with Ad GRP78 as compared to the control Ad beta-gal while there was no difference in ob/+ mice.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0010.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of HepG2 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane1: 1 ug/mL Lane2: 2 ug/mL](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0018.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of A549 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goatanti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane1:0.25 ug/mL Lane2: 0.5 ug/mL Lane3: 1 ug/mL](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0019.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 100 ng of human XBP-1 recombinant protein perlane. Antibodies: XBP-1 (A: 0.5 ug/mL, B: 1 ug/mL andC: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77253] - analysis of 4% paraformaldehydefixed Human Liver Tissue labeling XBP-1 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (green) and DAPI staining (blue](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77253-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77253] - analysis of 4% paraformaldehydefixed human pancreas tissue labeling XBP-1 with this antibody at20 ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77253-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77253] - analysis of 4% paraformaldehydefixed HepG2 cells labeling XBP-1 with this antibody at 20 ug/mL,followed by goat anti-rabbit IgG secondary antibody at1/500 dilution (green) and DAPI staining (blue)](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77253-img0016.jpg)
![Immunohistochemistry-Paraffin: XBP1 Antibody [NBP1-77253] - Analysis of paraffin-embedded RatLiver Tissue using anti-XBP-1 antibody at 5 ug/ml.Tissue was fixed with formaldehyde and blocked with 10%serum for 1 h at RT; antigen retrieval was by heatmediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-77253-img0011.jpg)
![Immunohistochemistry-Paraffin: XBP1 Antibody [NBP1-77253] - Analysis of paraffin-embeddedmouse testis tissue using anti-XBP-1 antibody at 2ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was byheat mediation with a citrate buffer (pH6). Samples wereincubated with primary antibody overnight at 4C. A goatanti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-77253-img0012.jpg)
![Western Blot: XBP1 Antibody [NBP1-77252] - Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation. Image from verified customer review.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77252-img0004.jpg "Western Blot: XBP1 Antibody [NBP1-77252] - Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77252] - HepG2 cells with XBP-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77252-img0002.jpg)
![Western Blot: XBP1 Antibody [NBP1-77252] - PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77252-img0003.jpg)
![Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP2-20917-img0002.jpg "Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Western Blot: XBP1 Antibody [NBP2-20917] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody [N1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP2-20917-img0001.jpg)
![Western Blot: XBP1 Antibody (3H1G4) [NBP1-75514] - analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/XBP1-Antibody-3H1G4-Western-Blot-NBP1-75514-img0001.jpg "Western Blot: XBP1 Antibody (3H1G4) [NBP1-75514] - analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 ug/mL.")
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody (3H1G4) [NBP1-75514] - XBP-1 in HepG2 cells with XBP-1 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/XBP1-Antibody-3H1G4-Immunocytochemistry-NBP1-75514-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody (3H1G4) [NBP1-75514] - XBP1 in HepG2 cells with XBP1 antibody at 4 ug/mL.](http://images.novusbio.com/fullsize/XBP1-Antibody-3H1G4-Immunofluorescence-NBP1-75514-img0003.jpg)
![Western Blot: XBP1 Antibody (S04-3E2) [NBP3-19927] - Western blot detection of XBP1 in HL-60, U2OS cell lysates using NBP3-19927 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.](http://images.novusbio.com/fullsize/XBP1-Antibody-S04-3E2-Western-Blot-NBP3-19927-img0001.jpg "Western Blot: XBP1 Antibody (S04-3E2) [NBP3-19927] - Western blot detection of XBP1 in HL-60, U2OS cell lysates using NBP3-19927 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.")
![Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBL1-17900] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.](http://images.novusbio.com/fullsize/XBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17900-img0002.jpg "Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBL1-17900] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.")
![Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBP2-08472] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.](http://images.novusbio.com/fullsize/XBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08472-img0001.jpg "Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBP2-08472] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.")
![Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBP2-09267] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.](http://images.novusbio.com/fullsize/XBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09267-img0001.jpg "Western Blot: XBP1 Overexpression Lysate (Adult Normal) [NBP2-09267] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XBP1.")
![ELISA: Human XBP1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38911] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38911_human-xbp1-ready-to-use-elisa-kit-colorimetric-2110202415334948.png "ELISA: Human XBP1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38911] - Standard Curve Reference")
![ELISA: Human XBP1 ELISA Kit (Colorimetric) [NBP3-38912] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38912_human-xbp1-elisa-kit-colorimetric-2110202415362323.png "ELISA: Human XBP1 ELISA Kit (Colorimetric) [NBP3-38912] - Standard Curve Reference")