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![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Western blot analysis of extracts of various cell lines, using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-6H2I9-Western-Blot-NBP3-16792-img0003.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Western blot analysis of extracts of various cell lines, using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Immunofluorescence analysis of U-2 OS cells using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-6H2I9-Immunocytochemistry-Immunofluorescence-NBP3-16792-img0002.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin-activating Enzyme/UBE1 Antibody (6H2I9) [NBP3-16792] - Immunohistochemistry of paraffin-embedded human esophageal using Ubiquitin-activating Enzyme/UBE1 Rabbit mAb (NBP3-16792) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-6H2I9-Immunohistochemistry-Paraffin-NBP3-16792-img0001.jpg)
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB600-472] - Shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye (TM) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel).](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Western-Blot-NB600-472-img0004.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB600-472] - Shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye (TM) 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel).")
![Immunohistochemistry: Ubiquitin-activating Enzyme/UBE1 Antibody [NB600-472] - Used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Immunohistochemistry-NB600-472-img0006.jpg)
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500] - Analysis using Anti-UBA1 antibody NBP2-76500 (A) shows similar pattern to independent antibody NBP1-90307 (B).](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Western-Blot-NBP2-76500-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500] - Analysis using Anti-UBA1 antibody NBP2-76500 (A) shows similar pattern to independent antibody NBP1-90307 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76500-img0002.jpg)
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP1-90307] - Analysis using Anti-UBA1 antibody NBP1-90307 (A) shows similar pattern to independent antibody NBP2-76500 (B).](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Western-Blot-NBP1-90307-img0016.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP1-90307] - Analysis using Anti-UBA1 antibody NBP1-90307 (A) shows similar pattern to independent antibody NBP2-76500 (B).")
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB100-58804] - Detection of Human and Mouse UBE1 on HeLa whole cell lysate using NB100-58804. UBE1 was also IPed efficiently by rabbit anti-UBE1 antibodies NB100-58805 and NB100-58806.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-Western-Blot-NB100-58804-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NB100-58804] - Detection of Human and Mouse UBE1 on HeLa whole cell lysate using NB100-58804. UBE1 was also IPed efficiently by rabbit anti-UBE1 antibodies NB100-58805 and NB100-58806.")
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (2B6) [H00007317-M02] - UBA1 monoclonal antibody (M02), clone 2B6. Analysis of UBA1 expression in HeLa.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-2B6-Western-Blot-H00007317-M02-img0002.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (2B6) [H00007317-M02] - UBA1 monoclonal antibody (M02), clone 2B6. Analysis of UBA1 expression in HeLa.")
![Sandwich ELISA: Ubiquitin-activating Enzyme/UBE1 Antibody (2B6) [H00007317-M02] - Detection limit for recombinant GST tagged UBA1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-2B6-Sandwich-ELISA-H00007317-M02-img0001.jpg)
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-20779] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ubiquitin-activating Enzyme/UBE1 antibody [N1N3] (NBP2-20779) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20779_rabbit-polyclonal-ubiquitin-activating-enzyme-ube1-antibody-91020242021911.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-20779] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ubiquitin-activating Enzyme/UBE1 antibody [N1N3] (NBP2-20779) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: UBE1 Overexpression Lysate (Adult Normal) [NBL1-17494] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE1.](http://images.novusbio.com/fullsize/UBE1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17494-img0002.jpg "Western Blot: UBE1 Overexpression Lysate (Adult Normal) [NBL1-17494] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE1.")