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![Western Blot: PIMT Antibody [NB100-2595] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-2595 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).](http://images.novusbio.com/fullsize/PIMT-Antibody-Western-Blot-NB100-2595-img0004.jpg "Western Blot: PIMT Antibody [NB100-2595] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-2595 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).")
![Immunohistochemistry: PIMT Antibody [NB100-2595] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PIMT used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunohistochemistry-NB100-2595-img0005.jpg)
![Immunohistochemistry-Paraffin: PIMT Antibody [NB100-2595] - FFPE section of human non-small cell lung cancer. Affinity purified rabbit anti-PIMT used at a dilution of 1:250.](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunohistochemistry-Paraffin-NB100-2595-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PIMT Antibody [NB100-2595] - Detection of human PIMT by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PIMT antibody NB100-2595 (lot NB100-2595-2) used for IP at 6 ug per reaction. PIMT was also immunoprecipitated by a previous lot of this antibody (lot NB100-2595-1) and rabbit anti-PIMT antibody NBP2-44294. For blotting immunoprecipitated PIMT, NB100-2595 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunoprecipitation-NB100-2595-img0006.jpg)
![Western Blot: PIMT Antibody [NBP2-44294] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NBP2-44294 was used for IP at 6 ug per reaction. PIMT was also immunoprecipitated by NB100-2595.](http://images.novusbio.com/fullsize/PIMT-Antibody-Western-Blot-NBP2-44294-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PIMT Antibody [NBP2-44294] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NBP2-44294 was used for IP at 6 ug per reaction. PIMT was also immunoprecipitated by NB100-2595.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: PIMT Antibody [NBP2-44294] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- PIMT used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunohistochemistry-NBP2-44294-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PIMT Antibody [NBP2-44294] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer.](http://images.novusbio.com/fullsize/PIMT-Antibody-Western-Blot-NBP2-44294-img0001.jpg)
![Western Blot: PIMT Antibody [NBP1-92271] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: NTERA-2 Medium](http://images.novusbio.com/fullsize/PIMT-Antibody-Western-Blot-NBP1-92271-img0004.jpg "Western Blot: PIMT Antibody [NBP1-92271] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: NTERA-2 Medium")
![Immunocytochemistry/Immunofluorescence: PIMT Antibody [NBP1-92271] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92271-img0005.jpg)
![Immunohistochemistry-Paraffin: PIMT Antibody [NBP1-92271] - Staining of human kidney shows strong nuclear positivity in tubule and glomerular cells.](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunohistochemistry-Paraffin-NBP1-92271-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PIMT Antibody [NBP2-56790] - Staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/PIMT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56790-img0001.jpg "Immunocytochemistry/Immunofluorescence: PIMT Antibody [NBP2-56790] - Staining of human cell line U-251 MG shows localization to cytosol.")
![Western Blot: PIMT Antibody [NBP1-54942] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.](http://images.novusbio.com/fullsize/PIMT-Antibody-Western-Blot-NBP1-54942-img0003.jpg "Western Blot: PIMT Antibody [NBP1-54942] - Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.")
![Sandwich ELISA: PIMT Antibody (3F1) [H00096764-M01] - Detection limit for recombinant GST tagged NCOA6IP is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIMT-Antibody-3F1-Sandwich-ELISA-H00096764-M01-img0002.jpg "Sandwich ELISA: PIMT Antibody (3F1) [H00096764-M01] - Detection limit for recombinant GST tagged NCOA6IP is approximately 0.3ng/ml as a capture antibody.")