Hu,
Eq,
Pm,
Mu,
Rt,
Po,
Ca,
Ch,
Pm,
Pm,
Ma-Op,
Pm-Or,
RM,
Xp,
ZeSpecies Glossary
Reactivity: Hu, Eq, Pm, Mu, Rt, Po, Ca, Ch, Pm, Pm, Ma-Op, Pm-Or, RM, Xp, Ze
Applications: WB, IHC
Host: Rabbit Polyclonal
Alt Catalog# IMG-6071A
Sample Size Available
Sizes: Sample Size Available
![Western Blot: TRBP Antibody [NBP2-24725] - Analysis of TRBP in human brain lysate in the 1) absence, 2) presence of immunizing peptide using this antibody at 6 ug/ml.](http://images.novusbio.com/fullsize/TRBP-Antibody-Western-Blot-NBP2-24725-img0001.jpg "Western Blot: TRBP Antibody [NBP2-24725] - Analysis of TRBP in human brain lysate in the 1) absence, 2) presence of immunizing peptide using this antibody at 6 ug/ml.")
![Immunohistochemistry-Paraffin: TRBP Antibody [NBP2-24725] - IHC staining of normal human brain using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/TRBP-Antibody-Immunohistochemistry-Paraffin-NBP2-24725-img0003.jpg)
![Immunohistochemistry-Paraffin: TRBP Antibody [NBP2-24725] - IHC staining of normal human brain using control (rabbit Ig) at 5 ug/ml.](http://images.novusbio.com/fullsize/TRBP-Antibody-Immunohistochemistry-Paraffin-NBP2-24725-img0002.jpg)
![Western Blot: TRBP Antibody (9E6C9) [NBP3-16753] - Western blot analysis of extracts of various cell lines, using TRBP Rabbit mAb (NBP3-16753) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/TRBP-Antibody-9E6C9-Western-Blot-NBP3-16753-img0006.jpg "Western Blot: TRBP Antibody (9E6C9) [NBP3-16753] - Western blot analysis of extracts of various cell lines, using TRBP Rabbit mAb (NBP3-16753) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: TRBP Antibody (9E6C9) [NBP3-16753] - Immunofluorescence analysis of U-2 OS cells using TRBP Rabbit mAb (NBP3-16753) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TRBP-Antibody-9E6C9-Immunocytochemistry-Immunofluorescence-NBP3-16753-img0005.jpg)
![Immunohistochemistry-Paraffin: TRBP Antibody (9E6C9) [NBP3-16753] - Immunohistochemistry of paraffin-embedded mouse pancreas using TRBP Rabbit mAb (NBP3-16753) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TRBP-Antibody-9E6C9-Immunohistochemistry-Paraffin-NBP3-16753-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRBP Antibody (9E6C9) [NBP3-16753] - Immunofluorescence analysis of NIH-3T3 cells using TRBP Rabbit mAb (NBP3-16753) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TRBP-Antibody-9E6C9-Immunocytochemistry-Immunofluorescence-NBP3-16753-img0004.jpg)
![Immunohistochemistry-Paraffin: TRBP Antibody (9E6C9) [NBP3-16753] - Immunohistochemistry of paraffin-embedded rat pancreas using TRBP Rabbit mAb (NBP3-16753) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TRBP-Antibody-9E6C9-Immunohistochemistry-Paraffin-NBP3-16753-img0001.jpg)
![Immunohistochemistry-Paraffin: TRBP Antibody (9E6C9) [NBP3-16753] - Immunohistochemistry of paraffin-embedded human placenta using TRBP Rabbit mAb (NBP3-16753) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TRBP-Antibody-9E6C9-Immunohistochemistry-Paraffin-NBP3-16753-img0002.jpg)
![Western Blot: TRBP Antibody (JE34-99) [NBP3-33046] - Western blot analysis of TRBP on different lysates with Rabbit anti-TRBP antibody (NBP3-33046) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: HEK-293 cell lysate Lane 5: A549 cell lysate Lane 6: Mouse testis tissue lysate Cell lysates/proteins at 15 ug/Lane. Tissue lysates/proteins at 30 ug/Lane. Predicted band size: 39kDa Observed band size: 39 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33046) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33046_rabbit-trbp-mab-je34-99-288202412493423.jpg "Western Blot: TRBP Antibody (JE34-99) [NBP3-33046] - Western blot analysis of TRBP on different lysates with Rabbit anti-TRBP antibody (NBP3-33046) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: HEK-293 cell lysate Lane 5: A549 cell lysate Lane 6: Mouse testis tissue lysate Cell lysates/proteins at 15 ug/Lane. Tissue lysates/proteins at 30 ug/Lane. Predicted band size: 39kDa Observed band size: 39 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33046) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TRBP Antibody (JE34-99) [NBP3-33046] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-TRBP antibody (NBP3-33046) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33046) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33046_rabbit-trbp-mab-je34-99-298202413585530.jpg)
![Immunocytochemistry/ Immunofluorescence: TRBP Antibody (JE34-99) [NBP3-33046] - Immunocytochemistry analysis of MCF7 cells labeling TRBP with Rabbit anti-TRBP antibody (NBP3-33046) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRBP antibody (NBP3-33046) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33046_rabbit-trbp-mab-je34-99-29202410484823.jpg)
![Flow Cytometry: TRBP Antibody (JE34-99) [NBP3-33046] - Flow cytometric analysis of MCF7 cells labeling TRBP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33046, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33046_rabbit-trbp-mab-je34-99-29202416372186.jpg)
![Immunocytochemistry/Immunofluorescence: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335] - Immunofluorescence Analysis of PFA-fixed HeLa cells using TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/TRBP-Antibody-PCRP-TARBP2-1E5-Immunocytochemistry-Immunofluorescence-NBP3-07335-img0003.jpg "Immunocytochemistry/Immunofluorescence: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335] - Immunofluorescence Analysis of PFA-fixed HeLa cells using TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).")
![Flow Cytometry: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335] - Flow Cytometric Analysis of PFA-fixed HeLa cells. TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/TRBP-Antibody-PCRP-TARBP2-1E5-Flow-Cytometry-NBP3-07335-img0002.jpg)
![Protein Array: TRBP Antibody (PCRP-TARBP2-1E5) [NBP3-07335] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TRBP Mouse Monoclonal Antibody (PCRP-TARBP2-1E5).](http://images.novusbio.com/fullsize/TRBP-Antibody-PCRP-TARBP2-1E5-Protein-Array-NBP3-07335-img0001.jpg)
![Western Blot: TRBP Antibody [NBP2-13411] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/TRBP-Antibody-Western-Blot-NBP2-13411-img0007.jpg "Western Blot: TRBP Antibody [NBP2-13411] - Analysis in human cell line HEK 293.")
![Immunocytochemistry/Immunofluorescence: TRBP Antibody [NBP2-13411] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/TRBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13411-img0008.jpg)
![Immunohistochemistry-Paraffin: TRBP Antibody [NBP2-13411] - Staining of human placenta shows strong positivity.](http://images.novusbio.com/fullsize/TRBP-Antibody-Immunohistochemistry-Paraffin-NBP2-13411-img0006.jpg)
![Western Blot: TRBP Antibody (SR1660) [NBP3-21937] - Western blot analysis using NBP3-21937 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-21937_rabbit-trbp-mab-sr1660-57202316112150.jpg "Western Blot: TRBP Antibody (SR1660) [NBP3-21937] - Western blot analysis using NBP3-21937 in Jurkat cell lysate.")
![Western Blot: TRBP Antibody (1D9) [H00006895-M03] - Analysis of TARBP2 expression in transfected 293T cell line by TARBP2 monoclonal antibody (M03), clone 1D9. Lane 1: TARBP2 transfected lysate (Predicted MW: 39 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TRBP-Antibody-1D9-Western-Blot-H00006895-M03-img0004.jpg "Western Blot: TRBP Antibody (1D9) [H00006895-M03] - Analysis of TARBP2 expression in transfected 293T cell line by TARBP2 monoclonal antibody (M03), clone 1D9. Lane 1: TARBP2 transfected lysate (Predicted MW: 39 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: TRBP Antibody (1D9) [H00006895-M03] - Analysis of TARBP2 transfected lysate using anti-TARBP2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TARBP2 purified rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/TRBP-Antibody-1D9-Western-Blot-H00006895-M03-img0002.jpg)
![ELISA: TRBP Antibody (1D9) [H00006895-M03] - Detection limit for recombinant GST tagged TARBP2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TRBP-Antibody-1D9-ELISA-H00006895-M03-img0005.jpg)
![Immunohistochemistry: TRBP Antibody [NBP2-49334] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/TRBP-Antibody-Immunohistochemistry-NBP2-49334-img0001.jpg "Immunohistochemistry: TRBP Antibody [NBP2-49334] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.")
![Western Blot: TRBP Overexpression Lysate (Adult Normal) [NBP2-05689] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRBP.](http://images.novusbio.com/fullsize/TRBP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05689-img0001.jpg "Western Blot: TRBP Overexpression Lysate (Adult Normal) [NBP2-05689] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRBP.")
![Western Blot: TRBP Overexpression Lysate (Adult Normal) [NBP2-05690] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRBP.](http://images.novusbio.com/fullsize/TRBP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05690-img0001.jpg "Western Blot: TRBP Overexpression Lysate (Adult Normal) [NBP2-05690] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TRBP.")
![Immunohistochemistry-Paraffin: TRBP Antibody [NBP2-98056] - Immunochemical staining of human TRBP in human placenta with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/TRBP-Antibody-Immunohistochemistry-Paraffin-NBP2-98056-img0002.jpg "Immunohistochemistry-Paraffin: TRBP Antibody [NBP2-98056] - Immunochemical staining of human TRBP in human placenta with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: TRBP Antibody [NBP2-98056] - Immunochemical staining of human TRBP in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/TRBP-Antibody-Immunohistochemistry-Paraffin-NBP2-98056-img0001.jpg)