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![Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.](http://images.novusbio.com/fullsize/Substance-P-Antibody-NC1-34-Immunohistochemistry-Frozen-NB100-65219-img0001.jpg "Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.")
![Western Blot: Preprotachykinin-1 Antibody [H00006863-D01P] - Analysis of Preprotachykinin-1 expression in human liver.](http://images.novusbio.com/fullsize/Substance-P-Antibody-Western-Blot-H00006863-D01P-img0002.jpg "Western Blot: Preprotachykinin-1 Antibody [H00006863-D01P] - Analysis of Preprotachykinin-1 expression in human liver.")
![Western Blot: Preprotachykinin-1 Antibody [H00006863-D01P] - Analysis of Preprotachykinin-1 expression in transfected 293T cell line by Preprotachykinin-1 polyclonal antibody.Lane 1: Preprotachykinin-1 transfected lysate(15.00 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Substance-P-Antibody-Western-Blot-H00006863-D01P-img0003.jpg)
![Immunohistochemistry: Substance P Antibody (JE59-73) [NBP3-32984] - Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rabbit anti-Substance P antibody (NBP3-32984) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32984) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32984_rabbit-substance-p-mab-je59-73-298202414115438.jpg "Immunohistochemistry: Substance P Antibody (JE59-73) [NBP3-32984] - Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue with Rabbit anti-Substance P antibody (NBP3-32984) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32984) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Preprotachykinin-1 Antibody [NBP1-82555] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neuronal cells.](http://images.novusbio.com/fullsize/Substance-P-Antibody-Immunohistochemistry-Paraffin-NBP1-82555-img0005.jpg "Immunohistochemistry-Paraffin: Preprotachykinin-1 Antibody [NBP1-82555] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neuronal cells.")
![ELISA: Human Neurokinin A ELISA Kit (Colorimetric) [NBP2-76744] - Samples were spiked with high concentrations of Human Neurokinin A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76744_human-neurokinin-a-elisa-kit-colorimetric-132202416143022.jpg "ELISA: Human Neurokinin A ELISA Kit (Colorimetric) [NBP2-76744] - Samples were spiked with high concentrations of Human Neurokinin A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Neurokinin A ELISA Kit (Colorimetric) [NBP2-76744] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76744_human-neurokinin-a-elisa-kit-colorimetric-4320249124862.jpg)
![Western Blot: Preprotachykinin-1 Overexpression Lysate [NBP2-10336] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Preprotachykinin-1.](http://images.novusbio.com/fullsize/Substance-P-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10336-img0001.jpg "Western Blot: Preprotachykinin-1 Overexpression Lysate [NBP2-10336] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Preprotachykinin-1.")
![Immunohistochemistry-Paraffin: Substance P Antibody (SR2325) [NBP3-27852] - Immunohistochemical analysis of paraffin-embedded human brain](http://images.novusbio.com/fullsize/nbp3-27852_rabbit-substance-p-mab-sr2325-7520249522010.jpg "Immunohistochemistry-Paraffin: Substance P Antibody (SR2325) [NBP3-27852] - Immunohistochemical analysis of paraffin-embedded human brain")
![SDS-Page: Recombinant Human, Preprotachykinin-1 His Protein [NBP2-22846] - SDS-PAGE of Preprotachykinin-1 His protein](http://images.novusbio.com/fullsize/Recombinant-Human-Substance-P-Protein-SDS-Page-NBP2-22846-img0002.jpg "SDS-Page: Recombinant Human, Preprotachykinin-1 His Protein [NBP2-22846] - SDS-PAGE of Preprotachykinin-1 His protein")