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![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Analysis in human skin and bone marrow tissues. Corresponding SUN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87396-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Analysis in human skin and bone marrow tissues. Corresponding SUN1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human fallopian tube, kidney, skin and testis using Anti-SUN1 antibody NBP1-87396 (A) shows similar protein distribution across tissues to independent antibody NBP1-87395 (B).](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87396-img0021.jpg)
![Western Blot: SUN1 Antibody [NBP1-87396] - Western blot analysis of protein expression levels in control and irradiated MCF7 and U2OS cells. Blot cuts showing signals of intact proteins. Note, reduction of SUN2 protein and near absence of H3K9me3 epigenetic modification in senescent cells (7 days post irradiation). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/4/999), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUN1-Antibody-Western-Blot-NBP1-87396-img0028.jpg)
![Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Examples of SUN1 a& nesprin-1 mislocalization in MCF7 and U2OS cells 24 h PI with 8 Gy of gamma-rays. 1a-Central slices through nucleus of MCF7 control show SUN1 & nesprin-1 in whole nucleus. 2-Convoluted nucleus of U2OS. 2a-Slice showing interrupted LA/C. 3-Convoluted nucleus of MCF7 containing 2 large MN, several blisters, and small MN bordered by LAC and filled by SUN1. 5-Tweens of MCF7 daughter nuclei connected by a thin anaphase bridge have fragmented SUN1 in the NE (arrows). 6-U2OS nucleus with amplified SUN1 and a clump (arrow) containing SUN1 and nesprin-1. Size bar indicates 5um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/4/999), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87396-img0027.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87396-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of A431 cells at 4ug/ml against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (red) and DAPI (blue), respectively.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87396-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87396] - Staining of human cell line A-431 shows positivity in nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87396-img0004.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human bone marrow shows low positivity in hematopoietic cells as expected.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87396-img0020.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human Fallopian tube shows moderate positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87396-img0022.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human kidney shows strong positivity in nuclear membrane in cells in glomeruli.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87396-img0023.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87396] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87396-img0025.jpg)
![Simple Western: SUN1 Antibody [NBP1-87396] - Simple Western lane view shows a specific band for SUN1 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SUN1-Antibody-Simple-Western-NBP1-87396-img0007.jpg)
![Simple Western: SUN1 Antibody [NBP1-87396] - Electropherogram image(s) of corresponding Simple Western lane view. SUN1 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/SUN1-Antibody-Simple-Western-NBP1-87396-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87396] - Examples of nuclear morphology defects in (A) MCF7 & (B) U2OS cell lines 24 h PI with the dose of 8 Gy of gamma -rays. (A) 1—An anaphase bridge linking the separating daughter nuclei, SUN1 (red), nesprin1 (green), 2—Convoluted nucleus with blisters (arrows), LA/C (green), emerin (red). 3—Fragmented nucleus represented by 3 large & numerous small micronuclei. SUN2 (red), nesprin-1 (green). (B) 1— A giant nucleus with small chromatin fragments & micronuclei (MN, arrows), SUN1 (red), nesprin-1 (green). 2—Nucleus with micronuclei, emerin (red), lamin A/C (green), arrows indicate MN containing emerin & heterochromatin (HC), 3—Convoluted nucleus with blisters (arrows), emerin (red), LA/C (green). 4—Fragmented nucleus, emerin (red), LA/C (green). 5—Honeycombed nucleus, emerin (red), LA/C (green), thick arrow shows interrupted NE enabling chromatin to leak out of the nucleus, reinforced layer of LA/C (green) & emerin (red), (thin arrows). Size bar indicates µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32316379), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87396_rabbit-polyclonal-sun1-antibody-310202416121982.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Analysis in human skin and bone marrow tissues. Corresponding SUN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87395-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Analysis in human skin and bone marrow tissues. Corresponding SUN1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human fallopian tube, kidney, skin and testis using Anti-SUN1 antibody NBP1-87395 (A) shows similar protein distribution across tissues to independent antibody NBP1-87396 (B).](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87395-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SUN1 Antibody [NBP1-87395] - Analysis using Anti-SUN1 antibody NBP1-87395 (A) shows similar pattern to independent antibody NBP1-87396 (B).](http://images.novusbio.com/fullsize/SUN1-Antibody-Western-Blot-NBP1-87395-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87395] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & vesicles.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87395-img0010.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87395-img0023.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human bone marrow shows low positivity in hematopoietic cells as expected.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87395-img0018.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human Fallopian tube shows moderate positivity in nuclear membrane in glandular cells.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87395-img0020.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human kidney shows strong positivity in nuclear membrane in cells in glomeruli.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87395-img0021.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.](http://images.novusbio.com/fullsize/SUN1-Antibody-Immunohistochemistry-Paraffin-NBP1-87395-img0022.jpg)
![Knockdown Validated: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-2120249362771.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - SUN1 antibody [HL1947] detects SUN1 protein at nuclear envelope by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SUN1 stained by SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-2120249393549.jpg)
![Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-212024934271.jpg)
![Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-2120249435513.jpg)
![Immunohistochemistry-Paraffin: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - SUN1 antibody [HL1947] detects SUN1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SUN1 stained by SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-2120249432854.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-2120249393526.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SUN1 Antibody (HL1947) - Azide and BSA Free [NBP3-25702] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SUN1 antibody [HL1947] (NBP3-25702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25702_rabbit-sun1-mab-hl1947-azide-and-bsa-free-212024940873.jpg)
![Western Blot: SUN1 Antibody [H00023353-B01P] - Analysis of UNC84A expression in transfected 293T cell line by UNC84A polyclonal antibody. Lane 1: UNC84A transfected lysate(28.38 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SUN1-Antibody-Western-Blot-H00023353-B01P-img0003.jpg "Western Blot: SUN1 Antibody [H00023353-B01P] - Analysis of UNC84A expression in transfected 293T cell line by UNC84A polyclonal antibody. Lane 1: UNC84A transfected lysate(28.38 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: SUN1 Antibody (HL1946) - Azide and BSA Free [NBP3-25701] - SUN1 antibody [HL1946] detects SUN1 protein at nuclear envelope by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SUN1 stained by SUN1 antibody [HL1946] (NBP3-25701) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25701_rabbit-sun1-mab-hl1946-azide-and-bsa-free-212024937153.jpg "Immunocytochemistry/Immunofluorescence: SUN1 Antibody (HL1946) - Azide and BSA Free [NBP3-25701] - SUN1 antibody [HL1946] detects SUN1 protein at nuclear envelope by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SUN1 stained by SUN1 antibody [HL1946] (NBP3-25701) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .")
![Immunocytochemistry/Immunofluorescence: SUN1 Antibody (HL1946) - Azide and BSA Free [NBP3-25701] - SUN1 antibody [HL1946] detects SUN1 protein at nuclear envelope by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SUN1 stained by SUN1 antibody [HL1946] (NBP3-25701) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25701_rabbit-sun1-mab-hl1946-azide-and-bsa-free-2120249355561.jpg)
![Western Blot: SUN1 Antibody (2D10) [H00023353-M01] - Detection against Immunogen (54.01 KDa) .](http://images.novusbio.com/fullsize/SUN1-Antibody-2D10-Western-Blot-H00023353-M01-img0001.jpg "Western Blot: SUN1 Antibody (2D10) [H00023353-M01] - Detection against Immunogen (54.01 KDa) .")