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![Western Blot: SPRY4 Antibody [NB100-2032] - Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 ug per lane. Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: a doublet band 35 kDa corresponding to human Sprouty-4. Other band(s): none.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Western-Blot-NB100-2032-img0006.jpg "Western Blot: SPRY4 Antibody [NB100-2032] - Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 ug per lane. Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: a doublet band 35 kDa corresponding to human Sprouty-4. Other band(s): none.")
![Immunohistochemistry-Paraffin: SPRY4 Antibody [NB100-2032] - Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Immunohistochemistry-Paraffin-NB100-2032-img0003.jpg)
![Immunohistochemistry-Paraffin: SPRY4 Antibody [NB100-2032] - Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Immunohistochemistry-Paraffin-NB100-2032-img0002.jpg)
![Western Blot: SPRY4 Antibody [NBP2-20479] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Western-Blot-NBP2-20479-img0002.jpg "Western Blot: SPRY4 Antibody [NBP2-20479] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479] - Paraffin-embedded mouse duodenum. SPRY4 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Immunohistochemistry-Paraffin-NBP2-20479-img0004.jpg)
![Immunohistochemistry-Paraffin: SPRY4 Antibody [NBP2-20479] - Paraffin-embedded U87 xenograft. SPRY4 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Immunohistochemistry-Paraffin-NBP2-20479-img0003.jpg)
![Western Blot: SPRY4 Antibody [NBP2-20479] - SPRY4 antibody detects SPRY4 protein by Western blot analysis.A. 30 ug NT2D1 whole cell lysate/extract12 % SDS-PAGESPRY4 antibody (NBP2-20479) dilution: 1:500](http://images.novusbio.com/fullsize/nbp2-20479_rabbit-polyclonal-spry4-antibody-91020242015726.jpg)
![Western Blot: SPRY4 Antibody (SR1553) [NBP3-21971] - Western blot analysis using NBP3-21971 in MCF7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21971_rabbit-spry4-mab-sr1553-57202316494267.jpg "Western Blot: SPRY4 Antibody (SR1553) [NBP3-21971] - Western blot analysis using NBP3-21971 in MCF7 cell lysate.")
![Western Blot: SPRY4 Antibody [NBP2-56160] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Western-Blot-NBP2-56160-img0002.jpg "Western Blot: SPRY4 Antibody [NBP2-56160] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: SPRY4 Antibody [NBP2-56160] - Staining of human cell line PC-3 shows localization to cytosol & the Golgi apparatus.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56160-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SPRY4 Antibody [NBP2-68941] - Staining of human cell line SK-MEL-30 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/SPRY4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68941-img0001.jpg "Immunocytochemistry/Immunofluorescence: SPRY4 Antibody [NBP2-68941] - Staining of human cell line SK-MEL-30 shows localization to the Golgi apparatus.")
![Western Blot: SPRY4 Overexpression Lysate (Adult Normal) [NBP2-06152] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPRY4.](http://images.novusbio.com/fullsize/SPRY4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06152-img0001.jpg "Western Blot: SPRY4 Overexpression Lysate (Adult Normal) [NBP2-06152] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPRY4.")
![SDS-Page: SPRY4 Protein [NBP2-22822]](http://images.novusbio.com/fullsize/SPRY4-Protein-SDS-Page-NBP2-22822-img0001.jpg "SDS-Page: SPRY4 Protein [NBP2-22822]")