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![Western Blot: RUNX1T1/ETO Antibody [NBP2-55747] - H2135 and H2087 non-small cell lung cancer cells showing baseline Runx1T1 expression versus Runx1T1 stable overexpression. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-Western-Blot-NBP2-55747-img0002.jpg "Western Blot: RUNX1T1/ETO Antibody [NBP2-55747] - H2135 and H2087 non-small cell lung cancer cells showing baseline Runx1T1 expression versus Runx1T1 stable overexpression. Image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody [NBP2-55747] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55747-img0001.jpg)
![Western Blot: RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free [NBP2-73956] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-RUNX1T1 monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-OTI1H1-Azide-and-BSA-Free-Western-Blot-NBP2-73956-img0004.jpg "Western Blot: RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free [NBP2-73956] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-RUNX1T1 monoclonal antibody (1:200).")
![Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free [NBP2-73956] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RUNX1T1.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-OTI1H1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73956-img0006.jpg)
![Western Blot: RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free [NBP2-73956] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUNX1T1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUNX1T1.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-OTI1H1-Azide-and-BSA-Free-Western-Blot-NBP2-73956-img0002.jpg)
![Western Blot: RUNX1T1/ETO Antibody (OTI1H1) - Azide and BSA Free [NBP2-73956] - Analysis of extracts (10ug) from 7 different cell lines by using anti-RUNX1T1 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-OTI1H1-Azide-and-BSA-Free-Western-Blot-NBP2-73956-img0003.jpg)
![Western Blot: RUNX1T1/ETO Antibody (1H1) [NBP2-02023] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUNX1T1/ETO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUNX1T1/ETO.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-1H1-Western-Blot-NBP2-02023-img0001.jpg "Western Blot: RUNX1T1/ETO Antibody (1H1) [NBP2-02023] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUNX1T1/ETO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUNX1T1/ETO.")
![Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody (1H1) [NBP2-02023] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RUNX1T1/ETO.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-1H1-Immunocytochemistry-Immunofluorescence-NBP2-02023-img0002.jpg)
![Western Blot: RUNX1T1/ETO Antibody (5A12) [H00000862-M01] - Analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 monoclonal antibody (M01), clone 5A12.Lane 1: RUNX1T1 transfected lysate(67.566 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-5A12-Western-Blot-H00000862-M01-img0007.jpg "Western Blot: RUNX1T1/ETO Antibody (5A12) [H00000862-M01] - Analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 monoclonal antibody (M01), clone 5A12.Lane 1: RUNX1T1 transfected lysate(67.566 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody (5A12) [H00000862-M01] - Analysis of monoclonal antibody to RUNX1T1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-5A12-Immunocytochemistry-Immunofluorescence-H00000862-M01-img0005.jpg)
![Sandwich ELISA: RUNX1T1/ETO Antibody (5A12) [H00000862-M01] - Detection limit for recombinant GST tagged RUNX1T1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-5A12-Sandwich-ELISA-H00000862-M01-img0006.jpg)
![Chromatin Immunoprecipitation: RUNX1T1/ETO Antibody [NBP2-59232] - ChIP assays were performed using SKNO-1 cells, the antibody against ETO and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 uL of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-Chromatin-Immunoprecipitation-NBP2-59232-img0002.jpg "Chromatin Immunoprecipitation: RUNX1T1/ETO Antibody [NBP2-59232] - ChIP assays were performed using SKNO-1 cells, the antibody against ETO and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 uL of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.")
![ELISA: RUNX1T1/ETO Antibody [NBP2-59232] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,300.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-ELISA-NBP2-59232-img0001.jpg)
![Immunohistochemistry-Paraffin: RUNX1T1/ETO Antibody [NBP3-09370] - Immunohistochemical analysis of formalin-fixed paraffin-embedded hepatocyte nuclei in human liver tissue using NBP3-09370 at a dilution of 1:100. Donkey anti-Rabbit-Cy3 used as a secondary antibody at a dilution of 1:200. 20X magnification.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-Immunohistochemistry-Paraffin-NBP3-09370-img0001.jpg "Immunohistochemistry-Paraffin: RUNX1T1/ETO Antibody [NBP3-09370] - Immunohistochemical analysis of formalin-fixed paraffin-embedded hepatocyte nuclei in human liver tissue using NBP3-09370 at a dilution of 1:100. Donkey anti-Rabbit-Cy3 used as a secondary antibody at a dilution of 1:200. 20X magnification.")
![Western Blot: RUNX1T1/ETO Antibody [NBP3-18685] - Figure 2A: Construction of GST-fusion proteins containing different parts of RUNX1T1/ETO constructed and run on 15% polyacrylamide gel. Figure 2B: Western blot performed on seven GST-fusion proteins containing different fragments of RUNX1T1/ETO (1-7) with antibody against RUNX1T1/ETO , 1:1000 in TBS-Tween, 5% skimmed milk. MW marker (in kDa) is shown on the left; location of the protein of interest indicated on the right. Antibody raised against RUNX1T1/ETO recognizes ETO part (lane 2) of fusion protein. Figure 2C: Western blot performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with antibody against RUNX1T1/ETO , diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Left, a MW marker is shown (in kDa). The location of RUNX1T1/ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-Western-Blot-NBP3-18685-img0002.jpg "Western Blot: RUNX1T1/ETO Antibody [NBP3-18685] - Figure 2A: Construction of GST-fusion proteins containing different parts of RUNX1T1/ETO constructed and run on 15% polyacrylamide gel. Figure 2B: Western blot performed on seven GST-fusion proteins containing different fragments of RUNX1T1/ETO (1-7) with antibody against RUNX1T1/ETO , 1:1000 in TBS-Tween, 5% skimmed milk. MW marker (in kDa) is shown on the left; location of the protein of interest indicated on the right. Antibody raised against RUNX1T1/ETO recognizes ETO part (lane 2) of fusion protein. Figure 2C: Western blot performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with antibody against RUNX1T1/ETO , diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Left, a MW marker is shown (in kDa). The location of RUNX1T1/ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.")
![ELISA: RUNX1T1/ETO Antibody [NBP3-18685] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against RUNX1T1/ETO , crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.](http://images.novusbio.com/fullsize/RUNX1T1-ETO Antibody-ELISA-NBP3-18685-img0001.jpg)
![SDS-Page: Recombinant Human RUNX1T1/ETO Protein [H00000862-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-RUNX1T1-ETO-Protein-SDS-Page-H00000862-Q01-img0001.jpg "SDS-Page: Recombinant Human RUNX1T1/ETO Protein [H00000862-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human RUNX1T1/ETO Protein [H00000862-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-RUNX1T1-ETO-Protein-SDS-Page-H00000862-P01-img0001.jpg "SDS-Page: Recombinant Human RUNX1T1/ETO Protein [H00000862-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: RUNX1T1/ETO Antibody [H00000862-D01P] - Analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 polyclonal antibody.Lane 1: RUNX1T1 transfected lysate(64.40 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-Western-Blot-H00000862-D01P-img0003.jpg "Western Blot: RUNX1T1/ETO Antibody [H00000862-D01P] - Analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 polyclonal antibody.Lane 1: RUNX1T1 transfected lysate(64.40 KDa).Lane 2: Non-transfected lysate.")
![Proximity Ligation Assay: RUNX1T1/ETO Antibody [H00000862-D01P] - Analysis of protein-protein interactions between RUNX1T1 and HDAC1. HeLa cells were stained with anti-RUNX1T1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-Proximity-Ligation-Assay-H00000862-D01P-img0002.jpg)
![Immunohistochemistry: RUNX1T1/ETO Antibody [NBP2-49644] - Staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells, neuropil was moderately stained.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Antibody-Immunohistochemistry-NBP2-49644-img0001.jpg "Immunohistochemistry: RUNX1T1/ETO Antibody [NBP2-49644] - Staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells, neuropil was moderately stained.")
![Western Blot: RUNX1T1/ETO Overexpression Lysate (Adult Normal) [NBL1-15625] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RUNX1T1/ETO.](http://images.novusbio.com/fullsize/RUNX1T1-ETO-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15625-img0002.jpg "Western Blot: RUNX1T1/ETO Overexpression Lysate (Adult Normal) [NBL1-15625] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RUNX1T1/ETO.")