| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3767-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50269-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37495-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37496-0.025ml | |||
![Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Western-Blot-NBP2-22527-img0006.jpg "Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunocytochemistry-Immunofluorescence-NBP2-22527-img0003.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0005.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0004.jpg)
![Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunoprecipitation-NBP2-22527-img0002.jpg)
![Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Western-Blot-NBP2-22526-img0001.jpg "Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0006.jpg)
![Immunohistochemistry: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 showing staining in the membrane of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunohistochemistry-NBP2-22526-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0003.jpg)
![Immunoprecipitation: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunoprecipitation-NBP2-22526-img0002.jpg)
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-10122024121014.jpg "Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-101220241224118.jpg)
![Western Blot: RAP1A Antibody [NBP3-35089] - Western blot analysis of lysates from wild type(WT) and RAP1A knockdown (KD) 293T cells, using [KO Validated] RAP1A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411552841.jpg)
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411592829.jpg)
![Western Blot: RAP1A Antibody [NBP3-35089] - Western blot analysis of various lysates using [KO Validated] RAP1A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411535893.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (RAP1 4C8/1) [NBP2-50269] - Staining of MEFs with anti-RAP1 [RAP1 4C8/1] antibody.](http://images.novusbio.com/fullsize/RAP1A-Antibody-RAP1-4C8-1-Immunofluorescence-NBP2-50269-img0001.jpg "Immunocytochemistry/Immunofluorescence: RAP1A Antibody (RAP1 4C8/1) [NBP2-50269] - Staining of MEFs with anti-RAP1 [RAP1 4C8/1] antibody.")
![Proximity Ligation Assay: RAP1A Antibody (3E1) [H00005906-M01] - Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/RAP1A-Antibody-3E1-Proximity-Ligation-Assay-H00005906-M01-img0002.jpg "Proximity Ligation Assay: RAP1A Antibody (3E1) [H00005906-M01] - Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: RAP1A Antibody (3E1) [H00005906-M01] - Detection limit for recombinant GST tagged RAP1A is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAP1A-Antibody-3E1-Sandwich-ELISA-H00005906-M01-img0003.jpg)
![Western Blot: Rap1A/B Antibody (6C7G0) [NBP3-16868] - Western blot analysis of extracts of various cell lines, using Rap1A/B Rabbit mAb (NBP3-16868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Rap1A-B-Antibody-6C7G0-Western-Blot-NBP3-16868-img0004.jpg "Western Blot: Rap1A/B Antibody (6C7G0) [NBP3-16868] - Western blot analysis of extracts of various cell lines, using Rap1A/B Rabbit mAb (NBP3-16868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Rap1A/B Antibody (6C7G0) [NBP3-16868] - Immunofluorescence analysis of U-2 OS cells using Rap1A/B Rabbit mAb (NBP3-16868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Rap1A-B-Antibody-6C7G0-Immunocytochemistry-Immunofluorescence-NBP3-16868-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Rap1A/B Antibody (6C7G0) [NBP3-16868] - Immunofluorescence analysis of C6 cells using Rap1A/B Rabbit mAb (NBP3-16868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Rap1A-B-Antibody-6C7G0-Immunocytochemistry-Immunofluorescence-NBP3-16868-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Rap1A/B Antibody (6C7G0) [NBP3-16868] - Immunofluorescence analysis of NIH-3T3 cells using Rap1A/B Rabbit mAb (NBP3-16868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Rap1A-B-Antibody-6C7G0-Immunocytochemistry-Immunofluorescence-NBP3-16868-img0002.jpg)
![Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-Western-Blot-NBP2-37495-img0003.jpg "Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: RAP1A Antibody (5F8G2) [NBP2-37495] - Flow cytometric analysis of HeLa cells using RAP1A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-Flow-Cytometry-NBP2-37495-img0004.jpg)
![Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 21 kDa)](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-Western-Blot-NBP2-37495-img0002.jpg)
![ELISA: RAP1A Antibody (5F8G2) [NBP2-37495] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-ELISA-NBP2-37495-img0001.jpg)
![Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-Western-Blot-NBP2-37496-img0003.jpg "Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: RAP1A Antibody (5F8) [NBP2-37496] - Flow cytometric analysis of A431 cells using RAP1A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-Flow-Cytometry-NBP2-37496-img0004.jpg)
![Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 43.2 kDa)](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-Western-Blot-NBP2-37496-img0002.jpg)
![ELISA: RAP1A Antibody (5F8) [NBP2-37496] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-ELISA-NBP2-37496-img0001.jpg)
![Western Blot: Rap1A/B Antibody (SR1333) [NBP3-22136] - Western blot analysis using NBP3-22136 in Hela cell lysate.](http://images.novusbio.com/fullsize/nbp3-22136_rabbit-rap1a-b-mab-sr1333-6720239533714.jpg "Western Blot: Rap1A/B Antibody (SR1333) [NBP3-22136] - Western blot analysis using NBP3-22136 in Hela cell lysate.")
![Western Blot: RAP1A Antibody [NBP1-32126] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAP1A antibody (NBP1-32126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32126_rabbit-polyclonal-rap1a-antibody-9102024201577.jpg "Western Blot: RAP1A Antibody [NBP1-32126] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAP1A antibody (NBP1-32126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: RAP1A Antibody [NBP1-32126] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAP1A antibody (NBP1-32126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32126_rabbit-polyclonal-rap1a-antibody-9102024205621.jpg)
![Western Blot: Rap1A/B Antibody (JE35-61) [NBP3-32893] - Western blot analysis of Rap1A/B on different lysates with Rabbit anti-Rap1A/B antibody (NBP3-32893) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32893) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32893_rabbit-rap1a-b-mab-je35-61-288202412461669.jpg "Western Blot: Rap1A/B Antibody (JE35-61) [NBP3-32893] - Western blot analysis of Rap1A/B on different lysates with Rabbit anti-Rap1A/B antibody (NBP3-32893) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32893) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Rap1A/B Antibody (JE35-61) [NBP3-32893] - Flow cytometric analysis of HEK-293 cells labeling Rap1A/B. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32893, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32893_rabbit-rap1a-b-mab-je35-61-29202416372167.jpg)