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![Western Blot: PYGL Antibody [NBP1-86182] - Analysis in human cell lines PC-3 and SK-MEL-30 using Anti-PYGL antibody. Corresponding PYGL RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/PYGL-Antibody-Western-Blot-NBP1-86182-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PYGL Antibody [NBP1-86182] - Analysis in human cell lines PC-3 and SK-MEL-30 using Anti-PYGL antibody. Corresponding PYGL RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PYGL Antibody [NBP1-86182] - Staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86182-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86182] - Staining of human bone marrow, cerebral cortex, liver and testis using Anti-PYGL antibody (A) NBP1-86182 shows similar protein distribution across tissues to independent antibody NBP1-86147 (B).](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86182-img0024.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86182] - Analysis in human liver and pancreas tissues. Corresponding PYGL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86182-img0018.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86182] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86182-img0019.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86182] - Staining of human cerebral cortex shows no positivity in neurons.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86182-img0020.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86182] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86182-img0021.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86182] - Staining of human pancreas shows no positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86182-img0022.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86182] - Staining of human testis shows no positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86182-img0023.jpg)
![Western Blot: PYGL Antibody [NBP1-86147] - Analysis in human cell lines PC-3 and SK-MEL-30 using anti-PYGL antibody. Corresponding PYGL RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/PYGL-Antibody-Western-Blot-NBP1-86147-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PYGL Antibody [NBP1-86147] - Analysis in human cell lines PC-3 and SK-MEL-30 using anti-PYGL antibody. Corresponding PYGL RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PYGL Antibody [NBP1-86147] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86147-img0006.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human pancreas shows very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86147-img0021.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Analysis in human liver and pancreas tissues. Corresponding PYGL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86147-img0014.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86147-img0015.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human cerebral cortex shows very weak positivity in neuronal cells.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86147-img0016.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86147-img0017.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human testis shows very weak positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86147-img0018.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human bone marrow, cerebral cortex, liver and testis using Anti-PYGL antibody (A) NBP1-86147 shows similar protein distribution across tissues to independent antibody NBP1-86182 (B).](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP1-86147-img0019.jpg)
![Immunocytochemistry/ Immunofluorescence: PYGL Antibody [NBP3-38314] - Immunofluorescence analysis of HeLa cells using PYGL Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38314_rabbit-pygl-pab-1012202411535818.jpg "Immunocytochemistry/ Immunofluorescence: PYGL Antibody [NBP3-38314] - Immunofluorescence analysis of HeLa cells using PYGL Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunoprecipitation: PYGL Antibody [NBP3-38314] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug PYGL antibody. Western blot was performed from the immunoprecipitate using PYGL antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38314_rabbit-pygl-pab-101220241153585.jpg)
![Western Blot: PYGL Antibody [NBP3-38314] - Western blot analysis of various lysates using PYGL Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38314_rabbit-pygl-pab-10122024120645.jpg)
![Immunohistochemistry: PYGL Antibody [NBP3-38314] - Immunohistochemistry analysis of paraffin-embedded Human liver using PYGL Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38314_rabbit-pygl-pab-101220241224110.jpg)
![Immunocytochemistry/ Immunofluorescence: PYGL Antibody [NBP3-38314] - Immunofluorescence analysis of HepG2 cells using PYGL Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38314_rabbit-pygl-pab-101220241214499.jpg)
![Western Blot: PYGL Antibody [NBP2-32246] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PYGL antibody NBP2-32246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PYGL-Antibody-Western-Blot-NBP2-32246-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PYGL Antibody [NBP2-32246] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PYGL antibody NBP2-32246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: PYGL Antibody [NBP2-32246] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from 293T cells. Antibodies: Affinity purified rabbit anti-PYGL antibody NBP2-32246 used for IP at 6 ug per reaction. PYGL was also immunoprecipitated by rabbit anti-PYGL antibody BL15846. For blotting immunoprecipitated PYGL, NBP2-32246 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunoprecipitation-NBP2-32246-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PYGL Antibody [NBP2-97708] - Immunofluorescence staining of PYGL in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PYGL polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97708-img0001.jpg "Immunocytochemistry/Immunofluorescence: PYGL Antibody [NBP2-97708] - Immunofluorescence staining of PYGL in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PYGL polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.")
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP2-97708] - Immunochemical staining of human PYGL in human liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PYGL-Antibody-Immunohistochemistry-Paraffin-NBP2-97708-img0002.jpg)
![Western Blot: PYGL Antibody [H00005836-D01P] - Analysis of PYGL expression in human liver.](http://images.novusbio.com/fullsize/PYGL-Antibody-Western-Blot-H00005836-D01P-img0001.jpg "Western Blot: PYGL Antibody [H00005836-D01P] - Analysis of PYGL expression in human liver.")
![Western Blot: PYGL Antibody [H00005836-D01P] - Analysis of PYGL expression in transfected 293T cell line by PYGL polyclonal antibody.Lane 1: PYGL transfected lysate(97.10 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PYGL-Antibody-Western-Blot-H00005836-D01P-img0002.jpg)
![ELISA: Human PYGL ELISA Kit (Colorimetric) [NBP2-82520] - Samples were spiked with high concentrations of Human PYGL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82520_human-pygl-elisa-kit-colorimetric-13220241613040.jpg "ELISA: Human PYGL ELISA Kit (Colorimetric) [NBP2-82520] - Samples were spiked with high concentrations of Human PYGL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human PYGL ELISA Kit (Colorimetric) [NBP2-82520] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82520_human-pygl-elisa-kit-colorimetric-43202485671.jpg)
![ELISA: Rat PYGL ELISA Kit (Colorimetric) [NBP2-82501] - Samples were spiked with high concentrations of Rat PYGL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82501_rat-pygl-elisa-kit-colorimetric-13220241613367.jpg "ELISA: Rat PYGL ELISA Kit (Colorimetric) [NBP2-82501] - Samples were spiked with high concentrations of Rat PYGL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat PYGL ELISA Kit (Colorimetric) [NBP2-82501] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82501_rat-pygl-elisa-kit-colorimetric-432024856723.jpg)
![ELISA: Mouse PYGL ELISA Kit (Colorimetric) [NBP2-82423] - Samples were spiked with high concentrations of Mouse PYGL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82423_mouse-pygl-elisa-kit-colorimetric-13220241625175.jpg "ELISA: Mouse PYGL ELISA Kit (Colorimetric) [NBP2-82423] - Samples were spiked with high concentrations of Mouse PYGL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse PYGL ELISA Kit (Colorimetric) [NBP2-82423] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82423_mouse-pygl-elisa-kit-colorimetric-432024973618.jpg)
![Immunohistochemistry-Paraffin: PYGL Antibody [NBP3-14458] - Immunohistochemistry of formalin-fixed, paraffin-embedded human liver after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PYGL Antibody-Immunohistochemistry-Paraffin-NBP3-14458-img0001.jpg "Immunohistochemistry-Paraffin: PYGL Antibody [NBP3-14458] - Immunohistochemistry of formalin-fixed, paraffin-embedded human liver after heat-induced antigen retrieval.")
![Western Blot: PYGL Overexpression Lysate (Adult Normal) [NBP2-08066] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PYGL.](http://images.novusbio.com/fullsize/PYGL-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08066-img0001.jpg "Western Blot: PYGL Overexpression Lysate (Adult Normal) [NBP2-08066] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PYGL.")
![SDS-Page: PYGL Recombinant Protein [NBP2-52066] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/PYGL-Recombinant-Protein-SDS-Page-NBP2-52066-img0001.jpg "SDS-Page: PYGL Recombinant Protein [NBP2-52066] - 15% SDS Page (3 ug)")