| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF847-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB847-SP | |||
| Carrier Free | IC847T-100UG | |||
| Carrier Free | IC847V-100UG | |||
| Carrier Free | IC847G-100UG | |||
| Carrier Free | IC847S-100UG | |||
| Carrier Free | IC847U-100UG | |||
| Carrier Free | IC847R-100UG | |||
| Carrier Free | IC847N-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6655-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2838-SP | |||
![Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Western-Blot-NBP3-15666-img0005.jpg "Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of C6 cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: PTEN Antibody (7O6F4) [NBP3-15666] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] PTEN Rabbit mAb (NBP3-15666) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Immunocytochemistry-Immunofluorescence-NBP3-15666-img0003.jpg)
![Immunohistochemistry-Paraffin: PTEN Antibody (7O6F4) [NBP3-15666] - Human breast cancer using [KO Validated] PTEN Rabbit mAb (NBP3-15666) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Immunohistochemistry-Paraffin-NBP3-15666-img0002.jpg)
![Western Blot: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts of various cell lines, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Western-Blot-NBP3-15666-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of extracts from normal (control) and PTEN knockout (KO) HeLa cells, using PTEN antibody (NBP3-15666) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Knockout-Validated-NBP3-15666-img0001.jpg)
![Immunoprecipitation: PTEN Antibody (7O6F4) [NBP3-15666] - Analysis of 300ug extracts of NIH/3T3 cells using 3ug PTEN antibody (NBP3-15666). Western blot was performed from the immunoprecipitate using PTEN antibody (NBP3-15666) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/PTEN-Antibody-7O6F4-Immunoprecipitation-NBP3-15666-img0006.jpg)
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTEN-[p-Thr366--p-Ser370]-Antibody-Western-Blot-NBP3-12919-img0004.jpg "Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/pS370) antibody (NBP3-12919) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)](http://images.novusbio.com/fullsize/PTEN-[p-Thr366--p-Ser370]-Antibody-Western-Blot-NBP3-12919-img0001.jpg)
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTEN-[p-Thr366--p-Ser370]-Antibody-Western-Blot-NBP3-12919-img0003.jpg)
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-12919_rabbit-pten-p-thr-366-p-ser-370-pab-9102024204126.jpg)
![Immunocytochemistry/ Immunofluorescence: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - PTEN antibody detects PTEN protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PTEN stained by PTEN antibody (NBP3-12919) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-12919_rabbit-pten-p-thr-366-p-ser-370-pab-91020242001095.jpg)
![Western Blot: PTEN Antibody [NB100-2229] - Detection of Human and Mouse PTEN on HeLa whole cell lysate using NB100-2229. PTEN was also IPed by rabbit anti-PTEN antibodies NB100-2227, NB100-2228 and another PTEN antibody.](http://images.novusbio.com/fullsize/PTEN-Antibody-Western-Blot-NB100-2229-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PTEN Antibody [NB100-2229] - Detection of Human and Mouse PTEN on HeLa whole cell lysate using NB100-2229. PTEN was also IPed by rabbit anti-PTEN antibodies NB100-2227, NB100-2228 and another PTEN antibody.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PTEN Antibody [NB100-2229] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PTEN used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PTEN-Antibody-Immunohistochemistry-NB100-2229-img0008.jpg)
![Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-288202412461629.jpg "Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTEN Antibody (JE51-96) [NBP3-32873] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32873) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32873) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29820241492644.jpg)
![Flow Cytometry: PTEN Antibody (JE51-96) [NBP3-32873] - Flow cytometric analysis of A431 cells labeling PTEN. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32873, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29202416372137.jpg)
![Immunohistochemistry: PTEN Antibody (SJ19-03) [NBP3-32872] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32872) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32872) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32872_rabbit-pten-mab-sj19-03-29820241492640.jpg "Immunohistochemistry: PTEN Antibody (SJ19-03) [NBP3-32872] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32872) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32872) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: PTEN Antibody (SJ19-03) [NBP3-32872] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32872, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32872_rabbit-pten-mab-sj19-03-2920241192394.jpg)
![Western Blot: PTEN Antibody (SJ19-03) [NBP3-32872] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32872) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-468 cell lysate (negative) Lane 3: A431 cell lysate Lane 4: HeLa cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32872_rabbit-pten-mab-sj19-03-3920248453545.jpg)