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![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0007.jpg "Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)")
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0001.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0002.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0003.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0004.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0005.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0006.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H3) [NBP2-45394] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PRMT5-Antibody-2H3-Immunohistochemistry-NBP2-45394-img0008.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19937] - Paraffin-embedded mouse colon. PRMT5 antibody [N3C3] dilution: 1:500.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19937-img0005.jpg "Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19937] - Paraffin-embedded mouse colon. PRMT5 antibody [N3C3] dilution: 1:500.")
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19937] - Immunohistochemical analysis of paraffin-embedded Mahlavu xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19937-img0002.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19937] - Paraffin-embedded mouse middle brain. PRMT5 antibody [N3C3] dilution: 1:500.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19937-img0004.jpg)
![Western Blot: PRMT5 Antibody [NBP2-19937] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [N3C3] (NBP2-19937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19937_rabbit-polyclonal-prmt5-antibody-91020242024610.jpg)
![Immunocytochemistry/ Immunofluorescence: PRMT5 Antibody [NBP2-19937] - PRMT5 antibody [N3C3] detects PRMT5 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PRMT5 stained by PRMT5 antibody [N3C3] (NBP2-19937) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-19937_rabbit-polyclonal-prmt5-antibody-91020242041233.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19937] - PRMT5 antibody [N3C3] detects PRMT5 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.PRMT5 stained by PRMT5 antibody [N3C3] (NBP2-19937) diluted at 1:2500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19937_rabbit-polyclonal-prmt5-antibody-910202420104420.jpg)
![Western Blot: PRMT5 Antibody [NB100-56641] - Western blot analysis of PRMT5 in cell lysates from HeLa cells using NB100-56641 at 1:500. Twenty microgram of HeLa cell lysate was loaded per well of a mini gel.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Western-Blot-NB100-56641-img0001.jpg "Western Blot: PRMT5 Antibody [NB100-56641] - Western blot analysis of PRMT5 in cell lysates from HeLa cells using NB100-56641 at 1:500. Twenty microgram of HeLa cell lysate was loaded per well of a mini gel.")
![Western Blot: PRMT5 Antibody [NB100-56641] - analysis of PRMT5 in A) human HeLa and B) mouse NIH 3T3 lysate using this antibody.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Western-Blot-NB100-56641-img0002.jpg)
![Western Blot: PRMT5 Antibody [NBP1-81701] - Western blot analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Western-Blot-NBP1-81701-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PRMT5 Antibody [NBP1-81701] - Western blot analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PRMT5 Antibody [NBP1-81701] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81701-img0008.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP1-81701] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP1-81701-img0001.jpg)
![Western Blot: PRMT5 Antibody [NBP1-81701] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Western-Blot-NBP1-81701-img0007.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19935] - Mouse testis. PRMT5 stained by PRMT5 antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19935-img0009.jpg "Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19935] - Mouse testis. PRMT5 stained by PRMT5 antibody [N1C1] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Chromatin Immunoprecipitation (ChIP): PRMT5 Antibody [NBP2-19935] - Cross-linked ChIP was performed with K562 chromatin extract and 5 ug of either control rabbit IgG or anti-PRMT5 antibody. The precipitated DNA was detected by PCR with primer set targeting to gamma-globin promoter.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-19935-img0005.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody [NBP2-19935] - MCF7 xenograft, using PRMT5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NBP2-19935-img0002.jpg)
![Immunoprecipitation: PRMT5 Antibody [NBP2-19935] - A. 35 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of PRMT5 protein by 4 ug PRMT5 antibody [N1C1] 7.5 % SDS-PAGE The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [N1C1] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunoprecipitation-NBP2-19935-img0007.jpg)
![Western Blot: PRMT5 Antibody [NBP2-19935] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [N1C1] (NBP2-19935) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19935_rabbit-polyclonal-prmt5-antibody-91020242091465.jpg)
![Western Blot: PRMT5 Antibody [NB110-40571] - Detection of Human and Mouse PRMT5 on HeLa whole cell lysate using NB110-40571. PRMT5 was also immunoprecipitated by another rabbit anti-PRMT5 antibodies and NB110-40570.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Western-Blot-NB110-40571-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PRMT5 Antibody [NB110-40571] - Detection of Human and Mouse PRMT5 on HeLa whole cell lysate using NB110-40571. PRMT5 was also immunoprecipitated by another rabbit anti-PRMT5 antibodies and NB110-40570.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PRMT5 Antibody [NB110-40571] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PRMT5 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PRMT5-Antibody-Immunohistochemistry-Paraffin-NB110-40571-img0005.jpg)
![Western Blot: PRMT5 Antibody (7K2G5) [NBP3-15758] - Western blot analysis of extracts of various cell lines, using PRMT5 antibody (NBP3-15758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PRMT5-Antibody-7K2G5-Western-Blot-NBP3-15758-img0003.jpg "Western Blot: PRMT5 Antibody (7K2G5) [NBP3-15758] - Western blot analysis of extracts of various cell lines, using PRMT5 antibody (NBP3-15758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758] - Immunohistochemistry of paraffin-embedded mouse testis using PRMT5 antibody (NBP3-15758) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PRMT5-Antibody-7K2G5-Immunohistochemistry-Paraffin-NBP3-15758-img0002.jpg)
![Immunohistochemistry-Paraffin: PRMT5 Antibody (7K2G5) [NBP3-15758] - Immunohistochemistry of paraffin-embedded rat testis using PRMT5 antibody (NBP3-15758) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PRMT5-Antibody-7K2G5-Immunohistochemistry-Paraffin-NBP3-15758-img0001.jpg)
![Flow Cytometry: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C136 (NBP3-20131, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20131_rabbit-prmt5-mab-rab-c136-chimeric-azide-and-bsa-free-2742023924283.png "Flow Cytometry: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C136 (NBP3-20131, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: PRMT5 Antibody (RAB-C136) - Chimeric - Azide and BSA Free [NBP3-20131] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C136 (NBP3-20131) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20131, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20131_rabbit-prmt5-mab-rab-c136-chimeric-azide-and-bsa-free-2742023924232.png)
![Western Blot: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGE PRMT5 antibody [3610] dilution: 1:1000.](http://images.novusbio.com/fullsize/PRMT5-Antibody-3610-Western-Blot-NBP2-43678-img0003.jpg "Western Blot: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGE PRMT5 antibody [3610] dilution: 1:1000.")
![Immunoprecipitation: PRMT5 Antibody (3610) [NBP2-43678] - Analysis of Raji whole cell extract A. 35 ug Raji whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of PRMT5 protein by 4 ug PRMT5 antibody [3610] 7.5 % SDS-PAGE The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [3610] diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/PRMT5-Antibody-3610-Immunoprecipitation-NBP2-43678-img0004.jpg)
![Western Blot: PRMT5 Antibody (3610) [NBP2-43678] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT5 antibody [GT3610] (NBP2-43678) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43678_mouse-monoclonal-prmt5-antibody-3610-91020242041294.jpg)
![Immunohistochemistry: PRMT5 Antibody (2H4) [NBP3-26367] - Image of PRMT5 Antibody (2H4) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26367_rabbit-prmt5-mab-2h4-26220241535578.jpg "Immunohistochemistry: PRMT5 Antibody (2H4) [NBP3-26367] - Image of PRMT5 Antibody (2H4) diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: PRMT5 Antibody (2H4) [NBP3-26367] - Positive Western Blot detected in: Raji whole cell lysate, Hela whole cell lysate, HL60 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate. All lanes: PRMT5 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 73, 72, 54, 68, 67 kDa Observed band size: 73 kDa](http://images.novusbio.com/fullsize/nbp3-26367_rabbit-prmt5-mab-2h4-28220241152517.jpg)
![Recombinant Human PRMT5 Protein [H00010419-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00010419-P01-1.jpg "Recombinant Human PRMT5 Protein [H00010419-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
