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![Western Blot: JM4 Antibody [NBP1-87886] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/JM4-Antibody-Western-Blot-NBP1-87886-img0004.jpg "Western Blot: JM4 Antibody [NBP1-87886] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: JM4 Antibody [NBP1-87886] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/JM4-Antibody-Immunohistochemistry-Paraffin-NBP1-87886-img0009.jpg)
![Western Blot: JM4 Antibody [NBP1-87886] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).](http://images.novusbio.com/fullsize/JM4-Antibody-Western-Blot-NBP1-87886-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: JM4 Antibody [NBP1-87886] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding PRAF2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/JM4-Antibody-Immunohistochemistry-Paraffin-NBP1-87886-img0005.jpg)
![Immunohistochemistry-Paraffin: JM4 Antibody [NBP1-87886] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/JM4-Antibody-Immunohistochemistry-Paraffin-NBP1-87886-img0006.jpg)
![Immunohistochemistry-Paraffin: JM4 Antibody [NBP1-87886] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/JM4-Antibody-Immunohistochemistry-Paraffin-NBP1-87886-img0007.jpg)
![Immunohistochemistry-Paraffin: JM4 Antibody [NBP1-87886] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/JM4-Antibody-Immunohistochemistry-Paraffin-NBP1-87886-img0008.jpg)
![Western Blot: JM4 Antibody [NBP3-35838] - Western blot analysis of various lysates using JM4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35838_rabbit-jm4-pab-1012202411592818.jpg "Western Blot: JM4 Antibody [NBP3-35838] - Western blot analysis of various lysates using JM4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![SDS-Page: Recombinant Human JM4 Protein [H00011230-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00011230-P01-1.jpg "SDS-Page: Recombinant Human JM4 Protein [H00011230-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: JM4 Overexpression Lysate (Adult Normal) [NBL1-14721] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JM4.](http://images.novusbio.com/fullsize/JM4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14721-img0002.jpg "Western Blot: JM4 Overexpression Lysate (Adult Normal) [NBL1-14721] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JM4.")