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![Western Blot: Polycystin 2 Antibody [NBP3-06363] - Anti-PKD2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HEK293 Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDa](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Western-Blot-NBP3-06363-img0001.jpg "Western Blot: Polycystin 2 Antibody [NBP3-06363] - Anti-PKD2 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HEK293 Whole Cell LysateLane C: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDa")
![Immunocytochemistry/Immunofluorescence: Polycystin 2 Antibody [NBP3-06363] - Immunofluorescence staining of PKD2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PKD2 polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06363-img0003.jpg)
![Immunohistochemistry: Polycystin 2 Antibody [NBP3-06363] - Immunochemical staining of human Polycystin 2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Immunohistochemistry-NBP3-06363-img0005.jpg)
![Immunohistochemistry-Paraffin: Polycystin 2 Antibody [NBP3-06363] - Immunochemical staining of human PKD2 in human gallbladder with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Immunohistochemistry-Paraffin-NBP3-06363-img0004.jpg)
![Immunoprecipitation: Polycystin 2 Antibody [NBP3-06363] - PKD2 was immunoprecipitated using:Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-PKD2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-PKD2 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 97 kDaObserved band size: 80 kDa](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Immunoprecipitation-NBP3-06363-img0002.jpg)
![Western Blot: Polycystin 2 Antibody (4C8) [H00005311-M01] - PKD2 monoclonal antibody (M01), clone 4C8. Analysis of PKD2 expression in human liver.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-4C8-Western-Blot-H00005311-M01-img0005.jpg "Western Blot: Polycystin 2 Antibody (4C8) [H00005311-M01] - PKD2 monoclonal antibody (M01), clone 4C8. Analysis of PKD2 expression in human liver.")
![Immunocytochemistry/Immunofluorescence: Polycystin 2 Antibody (4C8) [H00005311-M01] - Analysis of monoclonal antibody to PKD2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-4C8-Immunocytochemistry-Immunofluorescence-H00005311-M01-img0003.jpg)
![Sandwich ELISA: Polycystin 2 Antibody (4C8) [H00005311-M01] - Detection limit for recombinant GST tagged PKD2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-4C8-Sandwich-ELISA-H00005311-M01-img0004.jpg)
![Western Blot: Polycystin 2 Antibody [NBP2-19889] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Western-Blot-NBP2-19889-img0001.jpg "Western Blot: Polycystin 2 Antibody [NBP2-19889] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Paraffin: Polycystin 2 Antibody [NBP2-19889] - Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-19889-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Polycystin 2 Antibody [NBP2-19889] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Polycystin 2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19889_rabbit-polyclonal-polycystin-2-antibody-1610202419414548.jpg)
![Western Blot: Polycystin 2 Antibody [NBP1-30117] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Polycystin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Western-Blot-NBP1-30117-img0007.jpg "Western Blot: Polycystin 2 Antibody [NBP1-30117] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Polycystin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Polycystin 2 Antibody [NBP1-30117] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Polycystin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Western-Blot-NBP1-30117-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Polycystin 2 Antibody [NBP1-30117] - Detection of human Polycystin-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Polycystin-2 antibody NBP1-30117 used for IP at 3 ug per reaction. Polycystin-2 was also immunoprecipitated by another rabbit anti-Polycystin-2 antibody. For blotting immunoprecipitated Polycystin-2, NBP1-30117 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Immunoprecipitation-NBP1-30117-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Polycystin 2 Antibody [NBP1-80944] - Staining of human cell line BJ shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80944-img0004.jpg "Immunocytochemistry/Immunofluorescence: Polycystin 2 Antibody [NBP1-80944] - Staining of human cell line BJ shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Polycystin 2 Antibody [NBP1-80944] - Staining of human kidney shows positivity in a subset of renal tubules.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-80944-img0005.jpg)
![Western Blot: Polycystin 2 Antibody [NBP1-51975] - (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Western-Blot-NBP1-51975-img0001.jpg "Western Blot: Polycystin 2 Antibody [NBP1-51975] - (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![SDS-Page: Recombinant Human Polycystin 2 Protein [H00005311-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005311-Q01-1.jpg "SDS-Page: Recombinant Human Polycystin 2 Protein [H00005311-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Sandwich ELISA: Polycystin 2 Antibody (1G3) [H00005311-M02] - Detection limit for recombinant GST tagged PKD2 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-1G3-Sandwich-ELISA-H00005311-M02-img0002.jpg "Sandwich ELISA: Polycystin 2 Antibody (1G3) [H00005311-M02] - Detection limit for recombinant GST tagged PKD2 is 1 ng/ml as a capture antibody.")