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![Western Blot: PINX1 Antibody [NBP2-32265] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PINX1 antibody NBP2-32265 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PINX1-Antibody-Western-Blot-NBP2-32265-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PINX1 Antibody [NBP2-32265] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PINX1 antibody NBP2-32265 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PINX1 Antibody [NBP2-32265] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PINX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/PINX1-Antibody-Immunohistochemistry-NBP2-32265-img0003.jpg)
![Immunoprecipitation: PINX1 Antibody [NBP2-32265] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PINX1 antibody NBP2-32265 used for IP at 6 ug per reaction. PINX1 was also immunoprecipitated by rabbit anti-PINX1 antibody BL15294. For blotting immunoprecipitated PINX1, NBP2-32265 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PINX1-Antibody-Immunoprecipitation-NBP2-32265-img0002.jpg)
![Western Blot: PINX1 Antibody [NB100-890] - (2ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/PINX1-Antibody-Western-Blot-NB100-890-img0002.jpg "Western Blot: PINX1 Antibody [NB100-890] - (2ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Western Blot: PINX1 Antibody [NBP1-46134] - (0.3ug/ml) Analysis of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PINX1-Antibody-Western-Blot-NBP1-46134-img0002.jpg "Western Blot: PINX1 Antibody [NBP1-46134] - (0.3ug/ml) Analysis of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PINX1 Antibody [NBP1-83643] - Analysis in human cell line K562.](http://images.novusbio.com/fullsize/PINX1-Antibody-Western-Blot-NBP1-83643-img0008.jpg "Western Blot: PINX1 Antibody [NBP1-83643] - Analysis in human cell line K562.")
![Immunocytochemistry/Immunofluorescence: PINX1 Antibody [NBP3-17911] - Staining of human cell line HAP1 shows localization to nucleoplasm, nucleoli & mitochondria.](http://images.novusbio.com/fullsize/PINX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17911-img0001.jpg "Immunocytochemistry/Immunofluorescence: PINX1 Antibody [NBP3-17911] - Staining of human cell line HAP1 shows localization to nucleoplasm, nucleoli & mitochondria.")
![Western Blot: PINX1 Antibody [NBP2-19832] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PINX1-Antibody-Western-Blot-NBP2-19832-img0001.jpg "Western Blot: PINX1 Antibody [NBP2-19832] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: PINX1 Overexpression Lysate (Adult Normal) [NBL1-14430] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PINX1.](http://images.novusbio.com/fullsize/PINX1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14430-img0002.jpg "Western Blot: PINX1 Overexpression Lysate (Adult Normal) [NBL1-14430] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PINX1.")
![Western Blot: PINX1 Protein [NBC1-26375]](http://images.novusbio.com/fullsize/NBC1-26375.jpg "Western Blot: PINX1 Protein [NBC1-26375]")