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![Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining in human heart muscle and testis tissues.. Corresponding PDLIM5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Immunohistochemistry-Paraffin-NBP1-84843-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining in human heart muscle and testis tissues.. Corresponding PDLIM5 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis using Anti-PDLIM5 antibody NBP1-84843 (A) shows similar pattern to independent antibody NBP1-84842 (B).](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Western-Blot-NBP1-84843-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody [NBP1-84843] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84843-img0008.jpg)
![Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human testis shows no positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Immunohistochemistry-Paraffin-NBP1-84843-img0013.jpg)
![Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Western-Blot-NBP1-84843-img0007.jpg)
![Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Immunohistochemistry-Paraffin-NBP1-84843-img0009.jpg)
![Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Immunohistochemistry-Paraffin-NBP1-84843-img0011.jpg)
![Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Immunohistochemistry-Paraffin-NBP1-84843-img0012.jpg)
![Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - PDLIM5 monoclonal antibody (M01), clone 3E11-F6. Analysis of PDLIM5 expression in HepG2.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-3E11-F6-Western-Blot-H00010611-M01-img0007.jpg "Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - PDLIM5 monoclonal antibody (M01), clone 3E11-F6. Analysis of PDLIM5 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - Analysis of monoclonal antibody to PDLIM5 on A-431 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-3E11-F6-Immunocytochemistry-Immunofluorescence-H00010611-M01-img0005.jpg)
![Western Blot: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - Western Blot analysis of PDLIM5 expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize/PDLIM5-Antibody-3E11-F6-Western-Blot-H00010611-M01-img0001.jpg)
![Sandwich ELISA: PDLIM5 Antibody (3E11-F6) [H00010611-M01] - Detection limit for recombinant GST tagged PDLIM5 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-3E11-F6-Sandwich-ELISA-H00010611-M01-img0006.jpg)
![Western Blot: PDLIM5 Antibody [NBP1-84842] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Western-Blot-NBP1-84842-img0008.jpg "Western Blot: PDLIM5 Antibody [NBP1-84842] - Analysis in human cell line A-549.")
![Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody [NBP1-84842] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84842-img0006.jpg)
![Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84842] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Immunohistochemistry-Paraffin-NBP1-84842-img0001.jpg)
![Western Blot: PDLIM5 Antibody (1H10) [NBP2-01877] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-1H10-Western-Blot-NBP2-01877-img0002.jpg "Western Blot: PDLIM5 Antibody (1H10) [NBP2-01877] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5.")
![Flow Cytometry: PDLIM5 Antibody (1H10) [NBP2-01877] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDLIM5 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-1H10-Flow-Cytometry-NBP2-01877-img0001.jpg)
![Western Blot: PDLIM5 Antibody (1B5) [NBP2-46370] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-1B5-Western-Blot-NBP2-46370-img0001.jpg "Western Blot: PDLIM5 Antibody (1B5) [NBP2-46370] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5.")
![Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP3-10318] - Immunohistochemical analysis of formalin-fixed paraffin-embedded human liver tissue.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Immunohistochemistry-Paraffin-NBP3-10318-img0001.jpg "Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP3-10318] - Immunohistochemical analysis of formalin-fixed paraffin-embedded human liver tissue.")
![Western Blot: PDLIM5 Antibody (OTI1B5) - Azide and BSA Free [NBP2-73316] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-OTI1B5-Azide-and-BSA-Free-Western-Blot-NBP2-73316-img0001.jpg "Western Blot: PDLIM5 Antibody (OTI1B5) - Azide and BSA Free [NBP2-73316] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5.")
![Western Blot: PDLIM5 Antibody [NBP3-38177] - Western blot analysis of various lysates using PDLIM5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-38177_rabbit-pdlim5-pab-1012202411592876.jpg "Western Blot: PDLIM5 Antibody [NBP3-38177] - Western blot analysis of various lysates using PDLIM5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.")
![SDS-Page: Recombinant Human PDLIM5 Protein [H00010611-P01] - Quality control testing: 12.5% SDS-PAGE stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-PDLIM5-Protein-SDS-Page-H00010611-P01-img0002.jpg "SDS-Page: Recombinant Human PDLIM5 Protein [H00010611-P01] - Quality control testing: 12.5% SDS-PAGE stained with Coomassie Blue.")
![Western Blot: PDLIM5 Antibody [NBP2-19760] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PDLIM5-Antibody-Western-Blot-NBP2-19760-img0001.jpg "Western Blot: PDLIM5 Antibody [NBP2-19760] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: PDLIM5 Overexpression Lysate (Adult Normal) [NBL1-14257] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PDLIM5.](http://images.novusbio.com/fullsize/PDLIM5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14257-img0002.jpg "Western Blot: PDLIM5 Overexpression Lysate (Adult Normal) [NBL1-14257] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PDLIM5.")