| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-108SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2675-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2675-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95213-0.02ml | |||
![Western Blot: NTH1 Antibody [NB100-108] - Detection of NTH1 (37 kDa) from Hela & K562 cell extracts using NB100-108 (1:500). WB data courtesy of Mark Kelly, Indiana Univ.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-108-img0005.jpg "Western Blot: NTH1 Antibody [NB100-108] - Detection of NTH1 (37 kDa) from Hela & K562 cell extracts using NB100-108 (1:500). WB data courtesy of Mark Kelly, Indiana Univ.")
![Immunocytochemistry/Immunofluorescence: NTH1 Antibody [NB100-108] - NTH1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-108-img0006.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human colon adenocarcinoma using NTH1 antibody at 5ug/ml concentration (1:200 dilution). The primary antibody binding to NTH1/ NTHL1 antigen was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. The NTH1 antibody generated an expected nuclear cytoplasmic staining of NTH1 protein in colon cancer cells as well as the cells of tumor stroma including cancer associated fibroblasts. The nuclear staining of NTH1 was very strong in a sub-set of colon cancer cells.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0014.jpg)
![Western Blot: NTH1 Antibody [NB100-108] - Analysis of NTH1 expression in 1) HeLa, 2) A-431, 3) MCF7 whole cell lysates.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-108-img0002.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lymph node cancer using 5 ug/ml concentration of NTH1 antibody. The representative image shows strong nuclear and cytoplasmic positivity of NTH1 protein in the lymph node cancer cells.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0013.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human kidney cancer using 5 ug/ml concentration of NTH1 antibody. The renal cancer cells showed nuclear and cytoplasmic positivity for NTH1 protein, whereas, the tumor stroma was largely negative for immunostaining.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0012.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal cancer using 5 ug/ml concentration of NTH1 antibody. The rectal cancer cells as well as the goblet cells in glandular areas showed nuclear-cytoplasmic positivity for NTH1 protein. The cells of tumor stroma did not develop immunostaining for this protein.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0011.jpg)
![Immunohistochemistry-Paraffin: NTH1 Antibody [NB100-108] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human rectal cancer using 5 ug/ml concentration of NTH1 antibody. This representative image at high resolution depicts nuclear-cytoplasmic positivity for NTH1 protein in rectal cancer cells and the goblet cells. The cells of tumor stroma did not develop any immunostaining for this protein.](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunohistochemistry-Paraffin-NB100-108-img0010.jpg)
![Western Blot: NTH1 Antibody [NB100-302] - Analysis of A549 cells using NB100-302. A band is seen at 35 kDa representing NTH1.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NB100-302-img0003.jpg "Western Blot: NTH1 Antibody [NB100-302] - Analysis of A549 cells using NB100-302. A band is seen at 35 kDa representing NTH1.")
![Immunocytochemistry/Immunofluorescence: NTH1 Antibody [NB100-302] - The NTH1 antibody was tested in Hela cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/NTH1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-302-img0004.jpg)
![Immunohistochemistry: NTH1 Antibody (22H10) [NBP3-26456] - Image of NTH1 Antibody (22H10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26456_rabbit-nth1-mab-22h10-2722024831375.jpg "Immunohistochemistry: NTH1 Antibody (22H10) [NBP3-26456] - Image of NTH1 Antibody (22H10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: NTH1 Antibody (22H10) [NBP3-26456] - Positive Western Blot detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, PC-3 whole cell lysate. All lanes: NTH1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 35, 34, 33 kDa Observed band size: 35 kDa](http://images.novusbio.com/fullsize/nbp3-26456_rabbit-nth1-mab-22h10-28220241148582.jpg)
![Immunocytochemistry/Immunofluorescence: NTH1 Antibody (22H10) [NBP3-26456] - Staining of PC-3 cell with NTH1 Antibody (22H10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 604-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26456_rabbit-nth1-mab-22h10-28220241323620.jpg)
![Flow Cytometry: NTH1 Antibody (22H10) [NBP3-26456] - Overlay Peak curve showing HepG2 cells stained with NTH1 Antibody (22H10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26456_rabbit-nth1-mab-22h10-282202414312721.jpg)
![Western Blot: NTH1 Antibody (S08-8E9) [NBP3-19722] - Western blot detection of NTH1 in K562, Hela cell lysates using NBP3-19722 (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.](http://images.novusbio.com/fullsize/NTH1-Antibody-S08-8E9-Western-Blot-NBP3-19722-img0001.jpg "Western Blot: NTH1 Antibody (S08-8E9) [NBP3-19722] - Western blot detection of NTH1 in K562, Hela cell lysates using NBP3-19722 (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.")
![SDS-Page: Recombinant Human NTH1 His Protein [NBP1-72459] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-NTH1-His-Protein-SDS-Page-NBP1-72459-img0002.jpg "SDS-Page: Recombinant Human NTH1 His Protein [NBP1-72459] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![Western Blot: NTH1 Antibody [NBP2-95213] - Analysis of extracts of various cell lines, using NTH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/NTH1-Antibody-Western-Blot-NBP2-95213-img0001.jpg "Western Blot: NTH1 Antibody [NBP2-95213] - Analysis of extracts of various cell lines, using NTH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.")