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![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Western-Blot-NB200-209-img0019.jpg "<b>Biological Strategies Validation. </b>Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean +/- SEM. Image collected and cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NQO-1 Antibody (A180) [NB200-209] - NQO1 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunocytochemistry-Immunofluorescence-NB200-209-img0010.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunohistochemistry-Paraffin-NB200-209-img0014.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0018.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Western-Blot-NB200-209-img0007.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded human kidney tissue](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Immunohistochemistry-Paraffin-NB200-209-img0012.jpg)
![Flow (Intracellular): NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U87MG cells with NQO1 (A180) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Intracellular-NB200-209-img0015.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO-1 in Human A549 Cell Line by Flow Cytometry. Human A549 cell line was stained with Mouse Anti- NQO-1 Monoclonal Antibody (Catalog # NB200-209, filled histogram), or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0016.jpg)
![Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Flow-Cytometry-NB200-209-img0017.jpg)
![Simple Western: NQO-1 Antibody (A180) [NB200-209] - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NQO-1-Antibody-A180-Simple-Western-NB200-209-img0013.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of HO-1, NQO1 & GCLm proteins at 6, 12 & 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean ± SEM. Image collected & cropped by CiteAb from the following publication (//respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202415175255.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Equol attenuated ER stress by activating Nrf2 in vitro & in vivo.(A) HUVECs were incubated with different concentrations (1, 10 & 100 nM) of equol for 24 h before treatment with t-BHP (50 μM) for another 6 h. The expression of Nrf2 & NQO1 was detected by western blotting. (C) Total tissue lysates from the thoracic & abdominal aorta of apoE-/- mice with or without equol treatment were immunoblotted with anti-Nrf2 & anti-beta -actin antibodies. (E) Cells were transfected with Nrf2 siRNA for 5~6 h; the medium was then replaced with fresh culture medium, followed by incubation for another 24 h. Thereafter, the cells were treated with equol (100 nM) for 24 h & then were incubated with t-BHP (50 μM) for an additional 6 h. The cells were collected & lysed, & western blot analysis was performed. (B)(D)(F) The bar charts show the quantification of the indicated proteins. (G) HUVECs were transfected with Nrf2 siRNA & treated as described in (C), & cell apoptosis was assayed using the Cell Death Detection ELISAplus Kit. Values are presented as means ± SD. n = 3, *p < 0.05, **p < 0.01, ***p < 0.001 versus the control group, RC group or siRNA treated with no drug group; #p < 0.01, ##p < 0.001 versus the t-BHP-treated group & siRNA plus t-BHP treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27907038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416212320.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - S-(-)equol primarily up-regulated Nrf2 protein expression activating ARE-dependent response.(A) Whole EA.hy926 cells lysates of cells treated with 250 nM S-(-)equol, 500 nM daidzein, or 50 µM tBHQ for 24 h were subjected to immunoblot analysis with anti-Nrf2, anti-HO-1, anti-NQO1, & anti-beta -tubulin antibodies. (B) Total HUVECs lysates of cell treated with 250 nM S-(-)equol for 24 h were immunoblotted with anti-Nrf2 & anti-beta -tubulin antibodies. (C) EA.hy926 cells were transfected with plasmids containing an HA-tagged Nrf2 open reading frame & the renilla firefly luciferase gene. Medium was changed after 6 hours of incubation. Where indicated, extracts from HA-Nrf2 EA hy.92 cells were additionally probed with anti-HA antibody, anti-Nrf2, anti-HO-1, anti-NQO1 & anti-beta -tubulin antibodies. Values are means of three independent experiments with standard deviations represented by vertical bars. Mean values were significantly different compared to controls (*p<0.05). Total RNA was extracted from EA.hy926 cells or HUVECs treated as indicated, & Nrf2 (D), HO-1 (E), & NQO1 (F) mRNA was measured by real-time RT-PCR analysis. The values shown represent the mean ± SD obtained for three independent experiments (*p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205135.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - S-(-)equol primarily up-regulated Nrf2 protein expression activating ARE-dependent response.(A) Whole EA.hy926 cells lysates of cells treated with 250 nM S-(-)equol, 500 nM daidzein, or 50 µM tBHQ for 24 h were subjected to immunoblot analysis with anti-Nrf2, anti-HO-1, anti-NQO1, & anti-beta -tubulin antibodies. (B) Total HUVECs lysates of cell treated with 250 nM S-(-)equol for 24 h were immunoblotted with anti-Nrf2 & anti-beta -tubulin antibodies. (C) EA.hy926 cells were transfected with plasmids containing an HA-tagged Nrf2 open reading frame & the renilla firefly luciferase gene. Medium was changed after 6 hours of incubation. Where indicated, extracts from HA-Nrf2 EA hy.92 cells were additionally probed with anti-HA antibody, anti-Nrf2, anti-HO-1, anti-NQO1 & anti-beta -tubulin antibodies. Values are means of three independent experiments with standard deviations represented by vertical bars. Mean values were significantly different compared to controls (*p<0.05). Total RNA was extracted from EA.hy926 cells or HUVECs treated as indicated, & Nrf2 (D), HO-1 (E), & NQO1 (F) mRNA was measured by real-time RT-PCR analysis. The values shown represent the mean ± SD obtained for three independent experiments (*p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-31020241620372.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Phf2 protects the liver from high fat & high sucrose diet-induced fibrogenesis during obesity. GFP or Phf2 were overexpressed through AAV strategy specifically in the liver of 7-week-old male C57Bl/6 J mice. Mice were then fed with either a chow diet (NCD) or with a high fat & high sucrose diet (HFHSD) for 16 weeks. Mice were studied in the fed state. a Representative western blot analysis showing the contribution of Phf2 overexpression to the regulation of the PI3K/Akt signaling & pro-fibrogenic pathways in liver extracts (n = 15 per group). b (Left) Oral glucose tolerance test & insulin levels during the OGTT test. (right) Insulin tolerance test (n = 15 per group). c Liver sections stained with hematoxylin & eosin (H&E), trichrome masson & sirius red are shown. Scale bars = 100 μm (n = 10 per group). d Percentage of fibrotic area & percentage of apoptotic hepatocytes (n = 15 per group). e Relative oxidized GSSG content & measurement of Gpx activity (n = 10 per group). f Levels of carbonylated proteins (n = 10 mice per group). All error bars represent mean ± SEM. Statistical analyses were made using unpaired t-test (b) or Anova, followed by Bonferonni’s test (d, e). *P < 0.01 HFHSD/GFP compared to HFHSD/Phf2, $P < 0.01 HFHSD/GFP compared to NCD/GFP, £P < 0.01 HFHSD/Phf2 compared to HFHSD/GFP Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-04361-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-31020241621823.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Activation of Nrf2 signaling pathway by FST in RAW 264.7 mouse macrophage-like cells. Cells were treated with FST with or without 100 ng/mL RANKL for 5 days. (A) Total cellular proteins were isolated from cells & the expression levels of Nrf2 & its regulatory proteins were assessed by Western blot analysis. beta -actin was used as the internal control. (C) The expression of nuclear & cytosol Nrf2 were determined by Western blotting. Lamin B & beta -actin were used as internal controls for the nuclear & cytosolic fractions, respectively. The results shown are representative of three independent experiments. (B,D) Statistical analyses were conducted using analysis of variances between groups. * p < 0.05 & *** p < 0.0001 when compared to control. # p < 0.05 & ### p < 0.0001 when compared to RANKL treatment. RANKL: receptor of activator of nuclear factor kappa-B ligand; FST: fermented sea tangle extract; Nrf2: nuclear factor-erythroid 2-related factor 2; p-Nrf2: phosphorylated nuclear factor-erythroid 2-related factor 2; HO-1: heme oxygenase-1; NQO-1: NAD(P)H quinone oxidoreductase 1; +: cells treated the reagent; -: cells untreated the reagent. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31357503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205186.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Nrf2-pathway activation by hybrids: nuclear translocation & targets induction. (A) Nuclear cellular extracts of SH-SY5Y cells were treated for 3 hours with compounds at 5 µM, 500 nM, & 50 nM or with 20, 10, & 5 µM dimethyl fumarate (DMF). Nrf2 protein content in the nucleus was determined by Western blot. Anti-lamin A/C was used as a protein loading control. Results are shown as ratio Nrf2/lamin A/C ± SEM; *p < 0.05, **p < 0.01 & ****p < 0.0001 versus CTR; Dunnett’s multiple comparison test (F ratio = 6.797, n≥3). (B–C) RNA from total cellular extracts of SH-SY5Y cells, treated for 24 hours with 5 μM compounds or 20 μM DMF, were analyzed for NQO1 (B) & HO-1 (C) mRNA expression by RT-qPCR. GAPDH was used as housekeeping gene. Results are shown as mean ± SEM; *p < 0.05, **p < 0.01, & ***p < 0.001 versus CTR; Dunnett’s multiple comparison test (B, n≥3, F ratio = 10.44; C, n≥3, F ratio = 13.95). (D–E) Cellular extracts of SH-SY5Y cells treated for 24 hours with compounds at 5 μM or 20 μM DMF were analyzed for NQO1 (D) & HO-1 (E) protein levels by Western blot. Anti-actin was used as protein loading control. Results are shown as ratio (% of CTR) ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, & ****p < 0.0001 versus CTR; Dunnett’s multiple comparison test (D, n ≥ 3, F ratio = 5.144; E, n≥3, F ratio = 17.26). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32047434), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205137.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - The effects of PI3K/Akt & ER inhibitors on S-(-)equol-induced Nrf2 activation in endothelial cells.(A) EA.hy926 cells or HUVECs transfected w/ ARE-dependent firefly luciferase reporter gene (F) & the renilla firefly luciferase gene (R), & treated w/ 250 nM S-(-)equol in the presence or absence of 10 µM LY294002 or 100 nM ICI182,780 for 16 h. The potency of induction is expressed as the relative luminescence (R/F) measured using the dual luciferase reporter assay system (mean ± SD, n = 3). *p<0.05 versus w/ control group, #p<0.05 versus w/ S-(-)equol treated group. (B) EA.hy926 cells or HUVECs incubated w/ or w/out 10 µM LY294002 or 100 nM ICI182,780 for 30 min & then w/ or w/out 250 nM S-(-)equol or 500 nM daidzein for 24 h. Whole cell lysates then immunoblotted w/ antibodies against Nrf2, HO-1, NQO1, & beta -tubulin. Values are means of three independent experiments w/ standard deviations represented by vertical bars. Mean values significantly different compared w/ controls (*p<0.05). (C) EA.hy926 cells or HUVECs cotransfected w/ the HA-Nrf2 & renilla firefly luciferase expression plasmids treated w/ 250 nM S-(-)equol in the presence or absence of 10 µM LY294002 or 100 nM ICI182,780 for 16 h, & HA-Nrf2 localization analyzed by confocal microscopy. (D) EA.hy926 cells incubated w/ 250 nM S-(-)equol, 500 nM daidzein w/ or w/out 10 µM LY294002 or 100 nM ICI182,780 for 16 h & then immunostained w/ an antibody against Nrf2, & Nrf2 localization analyzed by confocal microscopy. Values (intensity of nuclear versus cytoplasmic) are means of counting 100 cells w/ standard deviations represented by vertical bars. *p<0.05 versus w/ control group, #p<0.05 versus w/ S-(-)equol treated group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416205110.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - S-(-)equol primarily up-regulated Nrf2 protein expression activating ARE-dependent response.(A) Whole EA.hy926 cells lysates of cells treated with 250 nM S-(-)equol, 500 nM daidzein, or 50 µM tBHQ for 24 h were subjected to immunoblot analysis with anti-Nrf2, anti-HO-1, anti-NQO1, & anti-beta -tubulin antibodies. (B) Total HUVECs lysates of cell treated with 250 nM S-(-)equol for 24 h were immunoblotted with anti-Nrf2 & anti-beta -tubulin antibodies. (C) EA.hy926 cells were transfected with plasmids containing an HA-tagged Nrf2 open reading frame & the renilla firefly luciferase gene. Medium was changed after 6 hours of incubation. Where indicated, extracts from HA-Nrf2 EA hy.92 cells were additionally probed with anti-HA antibody, anti-Nrf2, anti-HO-1, anti-NQO1 & anti-beta -tubulin antibodies. Values are means of three independent experiments with standard deviations represented by vertical bars. Mean values were significantly different compared to controls (*p<0.05). Total RNA was extracted from EA.hy926 cells or HUVECs treated as indicated, & Nrf2 (D), HO-1 (E), & NQO1 (F) mRNA was measured by real-time RT-PCR analysis. The values shown represent the mean ± SD obtained for three independent experiments (*p<0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24260155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416212388.jpg)
![Western Blot: NQO-1 Antibody (A180) [NB200-209] - Activation of Nrf2 signaling pathway by FST in RAW 264.7 mouse macrophage-like cells. Cells were treated with FST with or without 100 ng/mL RANKL for 5 days. (A) Total cellular proteins were isolated from cells & the expression levels of Nrf2 & its regulatory proteins were assessed by Western blot analysis. beta -actin was used as the internal control. (C) The expression of nuclear & cytosol Nrf2 were determined by Western blotting. Lamin B & beta -actin were used as internal controls for the nuclear & cytosolic fractions, respectively. The results shown are representative of three independent experiments. (B,D) Statistical analyses were conducted using analysis of variances between groups. * p < 0.05 & *** p < 0.0001 when compared to control. # p < 0.05 & ### p < 0.0001 when compared to RANKL treatment. RANKL: receptor of activator of nuclear factor kappa-B ligand; FST: fermented sea tangle extract; Nrf2: nuclear factor-erythroid 2-related factor 2; p-Nrf2: phosphorylated nuclear factor-erythroid 2-related factor 2; HO-1: heme oxygenase-1; NQO-1: NAD(P)H quinone oxidoreductase 1; +: cells treated the reagent; -: cells untreated the reagent. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31357503), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-209_mouse-monoclonal-nqo-1-antibody-a180-310202416203710.jpg)
![Knockdown Validated: NQO-1 Antibody [NBP1-31355] - (b) Immunohistochemical analyses of RKO/pNQO1, RKO/shCont, and RKO/pshNQO1 xenograft tumours. The sections were stained for NQO1. Scale bar, 50 um. (c) Quantification of NQO1 in RKO/pNQO1, RKO/shCont and RKO/pshNQO1 xenograft tumours (n=3 each group). n=5 in each tumour. Two-tail t-test. *P<0.05. All error bars represent the mean +/- s.e.m. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13593) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Knockdown-Validated-NBP1-31355-img0025.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: NQO-1 Antibody [NBP1-31355] - (b) Immunohistochemical analyses of RKO/pNQO1, RKO/shCont, and RKO/pshNQO1 xenograft tumours. The sections were stained for NQO1. Scale bar, 50 um. (c) Quantification of NQO1 in RKO/pNQO1, RKO/shCont and RKO/pshNQO1 xenograft tumours (n=3 each group). n=5 in each tumour. Two-tail t-test. *P<0.05. All error bars represent the mean +/- s.e.m. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13593) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: NQO-1 Antibody [NBP1-31355] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NQO1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP1-31355-img0021.jpg)
![Immunohistochemistry: NQO-1 Antibody [NBP1-31355] - IHC detection of high-level expression of NQO1 (n=11) compared with the low-level expression of NQO1 (n=9). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13593) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-NBP1-31355-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NBP1-31355] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31355-img0016.jpg)
![Western Blot: NQO-1 Antibody [NBP1-31355] - A. 50 ug mouse heart lysate/extract 12% SDS-PAGE NQO1 antibody [C2C3], C-term dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP1-31355-img0017.jpg)
![Western Blot: NQO-1 Antibody [NBP1-31355] - A. 50 ug rat heart lysate/extract 12% SDS-PAGE NQO1 antibody [C2C3], C-term (GTX100235) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP1-31355-img0018.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-31355] - Human rectal carcinoma. NQO1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31355-img0019.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-31355] - Mouse kidney. NQO1 stained by NQO1 antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31355-img0022.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-31355] - Mouse kidney. NQO1 stained by NQO1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31355-img0023.jpg)
![Immunohistochemistry: NQO-1 Antibody [NBP1-31355] - NQO1 promotesin vivo tumour growth.(a) RKO/pNQO1, RKO/shCont & RKO/pshNQO1 cells injected subcutaneously into right flank of athymic, 7-week-old female BALB/C nude mice, & tumour growth assessed. Tumour volume (TV) calculated by using following formula: TV=length × (width)2 × 0.5. Each group contained 12 animals. (**P<0.01 w/ unpaired t-test). (b) Immunohistochemical analyses of RKO/pNQO1, RKO/shCont, & RKO/pshNQO1 xenograft tumours. The sections stained for NQO1, HIF-1 alpha , proliferation (Ki67) & apoptosis (CC3) using 3,3′-DAB. Scale bar, 50 μm. (c) Quantification of NQO1, proliferative marker Ki67 & apoptotic marker CC3 in RKO/pNQO1, RKO/shCont & RKO/pshNQO1 xenograft tumours (n=3 each group). n=5 in each tumour. Two-tail t-test. **P<0.01, *P<0.05. #, not significant. All error bars represent mean±s.e.m. (d) RKO/pshCont1/pshCont2, RKO/pNQO1/pshCont2 & RKO/pNQO1/pshHIF-1 alpha injected subcutaneously into right flank of athymic, 7-week-old female BALB/C nude mice, & tumour growth assessed. Tumour volume (TV) calculated by using following formula: TV=length × (width)2 × 0.5. Each group contained 10 animals (*P<0.05 w/ unpaired t-test). (e) Immunohistochemical analyses of RKO/pshCont1/pshCont2, RKO/pNQO1/pshCont2 & RKO/pNQO1/pshHIF-1 alpha xenograft tumours for HIF-1 alpha , proliferation (Ki67), apoptosis (CC3) & vasculature (CD34) using 3,3′-DAB. Arrowheads denote blood vessels. Scale bar, 50 μm. (f) RKO/pNQO1/pshCont or RKO/pNQO1/pshHIF-1 alpha xenograft tumours (n=3–5 each group) quantified for proliferation (Ki67), apoptosis (CC3) & vascularization (CD34). n=5 in each tumour. Two tailed t-test. **P<0.01, *P<0.05. #, not significant. All error bars represent mean±s.e.m. (g) Schematic model showing how NQO1 stabilizes HIF-1 alpha in cancer cells. Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/27966538), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31355_rabbit-polyclonal-nqo-1-antibody-310202416154614.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0005.jpg "Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of U251 (A) and HepG2 (B) cell lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NB100-1005] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1005-img0001.jpg)
![Immunohistochemistry: NQO-1 Antibody [NB100-1005] - (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-NB100-1005-img0002.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Rat Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0003.jpg)
![Western Blot: NQO-1 Antibody [NB100-1005] - (0.3ug/ml) staining of Human Kidney (A), Lung (B) and Duodenum (C) lysate. (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NB100-1005-img0004.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining in human stomach and lymph node tissues.. Corresponding NQO1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining in human stomach and lymph node tissues.. Corresponding NQO1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NQO-1 Antibody [NBP1-85223] - Analysis in human cell lines A-549 and HEK293 using Anti-NQO1 antibody. Corresponding NQO1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP1-85223-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody [NBP1-85223] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85223-img0008.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0017.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0015.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0016.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody [NBP1-85223] - Staining of human lymph node shows no positivity in non - germinal center cells as expected.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85223-img0018.jpg)
![Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody (M01), clone 1E3-A6.Lane 1: NQO1 transfected lysate(30.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Western-Blot-H00001728-M01-img0013.jpg "Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody (M01), clone 1E3-A6.Lane 1: NQO1 transfected lysate(30.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of monoclonal antibody to NQO1 on HepG2 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Immunocytochemistry-Immunofluorescence-H00001728-M01-img0008.jpg)
![Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 over-expressed 293 cell line, cotransfected with NQO1 Validated Chimera RNAi ( Cat # H00001728-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NQO1 monoclonal antibody (M01), clone 1E3-A6 (Cat # H00001728-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Western-Blot-H00001728-M01-img0010.jpg)
![Western Blot: NQO-1 Antibody (1E3-A6) [H00001728-M01] - NQO1 monoclonal antibody (M01), clone 1E3-A6 Analysis of NQO1 expression in HepG2.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Western-Blot-H00001728-M01-img0012.jpg)
![Immunoprecipitation: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Analysis of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NQO1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Immunoprecipitation-H00001728-M01-img0009.jpg)
![Sandwich ELISA: NQO-1 Antibody (1E3-A6) [H00001728-M01] - Detection limit for recombinant GST tagged NQO1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NQO-1-Antibody-1E3-A6-Sandwich-ELISA-H00001728-M01-img0011.jpg)
![NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of extracts of various lysates, using NQO1 antibody at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15793_rabbit-nqo-1-mab-1o2n3-knockout-validated-4520238114..jpg "NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of extracts of various lysates, using NQO1 antibody at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of extracts from wild type(WT) and NQO1 knockdown (KD) HeLa cells, using NQO1 antibody at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15793_rabbit-nqo-1-mab-1o2n3-knockdown-validated-45202381317..jpg)
![NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of Rat stomach using NQO1 antibody at dilution of 1:300 (40x lens).Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15793_rabbit-nqo-1-mab-1o2n3-immunohistochemistry-4520238153..jpg)
![NQO-1 Antibody (1O2N3) [NBP3-15793] -Analysis of Mouse stomach using NQO1 antibody at dilution of 1:300 (40x lens).Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15793_rabbit-nqo-1-mab-1o2n3-immunohistochemistry-45202381637..jpg)
![Western Blot: NQO-1 Antibody (13A1) [NBP2-80395] - Analysis of NQO-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: SiHa cell lysate, untreated Lane 2: A549 cell lysate, untreated](http://images.novusbio.com/fullsize/NQO-1-Antibody-13A1-Western-Blot-NBP2-80395-img0002.jpg "Western Blot: NQO-1 Antibody (13A1) [NBP2-80395] - Analysis of NQO-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: SiHa cell lysate, untreated Lane 2: A549 cell lysate, untreated")
![Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-NQO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/NQO-1-Antibody-13A1-Immunohistochemistry-Paraffin-NBP2-80395-img0003.jpg)
![Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395] - Analysis in human kidney tissue using NQO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with](http://images.novusbio.com/fullsize/NQO-1-Antibody-13A1-Immunohistochemistry-Paraffin-NBP2-80395-img0001.jpg)
![Western Blot: NQO-1 Antibody [NBP2-19573] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NQO-1-Antibody-Western-Blot-NBP2-19573-img0002.jpg "Western Blot: NQO-1 Antibody [NBP2-19573] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: NQO1 Overexpression Lysate (Adult Normal) [NBL1-13761] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NQO1.](http://images.novusbio.com/fullsize/NQO1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13761-img0002.jpg "Western Blot: NQO1 Overexpression Lysate (Adult Normal) [NBL1-13761] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NQO1.")