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![Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - Wild-type (WT) and NEK7 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25575_rabbit-nek7-mab-hl1348-azide-and-bsa-free-2120249371545.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - Wild-type (WT) and NEK7 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25575_rabbit-nek7-mab-hl1348-azide-and-bsa-free-2120249342794.jpg)
![Western Blot: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25575_rabbit-nek7-mab-hl1348-azide-and-bsa-free-2120249355557.jpg)
![Immunocytochemistry/Immunofluorescence: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25575_rabbit-nek7-mab-hl1348-azide-and-bsa-free-2120249371568.jpg)
![Immunohistochemistry-Paraffin: NEK7 Antibody (HL1348) - Azide and BSA Free [NBP3-25575] - NEK7 antibody [HL1348] detects NEK7 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NEK7 stained by NEK7 antibody [HL1348] (NBP3-25575) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25575_rabbit-nek7-mab-hl1348-azide-and-bsa-free-2120249351347.jpg)
![Western Blot: NEK7 Antibody [NBP1-52115] - (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NEK7-Antibody-Western-Blot-NBP1-52115-img0003.jpg "Western Blot: NEK7 Antibody [NBP1-52115] - (0.05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NEK7 Antibody [NBP1-52115] - 3.75ug/ml staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NEK7-Antibody-Immunohistochemistry-Paraffin-NBP1-52115-img0004.jpg)
![Western Blot: NEK7 Antibody [NBP1-68866] - Analysis of Mouse Lung (A), Mouse Testis (B), Rat Lung (C) and Rat Testis (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NEK7-Antibody-Western-Blot-NBP1-68866-img0002.jpg "Western Blot: NEK7 Antibody [NBP1-68866] - Analysis of Mouse Lung (A), Mouse Testis (B), Rat Lung (C) and Rat Testis (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: NEK7 Antibody [NBP1-68866] - NBP1-68866 (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NEK7-Antibody-Western-Blot-NBP1-68866-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NEK7 Antibody [NBP1-86444] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NEK7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86444-img0006.jpg "Immunocytochemistry/Immunofluorescence: NEK7 Antibody [NBP1-86444] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: NEK7 Antibody [NBP1-86444] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NEK7-Antibody-Immunohistochemistry-Paraffin-NBP1-86444-img0005.jpg)
![Western Blot: NEK7 Antibody [NBP3-37907] - Western blot analysis of various lysates using NEK7 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-37907_rabbit-nek7-pab-912202415161877.jpg "Western Blot: NEK7 Antibody [NBP3-37907] - Western blot analysis of various lysates using NEK7 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.")
![Western Blot: NEK7 Antibody [NBP3-37907] - Western blot analysis of various lysates using NEK7 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-37907_rabbit-nek7-pab-912202415152813.jpg)
![Immunohistochemistry;NEK7 Antibody[NBP3-30658]Detection of human NEK7 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NEK7 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30659_rabbit-nek7-pab-78202410153511.jpg "Immunohistochemistry;NEK7 Antibody[NBP3-30658]Detection of human NEK7 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NEK7 used at a dilution of 1:1,000 . Detection: DAB")
![Western Blot: NEK7 Antibody (7Y3B10) [NBP3-15936] - Western blot analysis of extracts of various cell lines, using NEK7 Rabbit mAb (NBP3-15936) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/NEK7-Antibody-7Y3B10-Western-Blot-NBP3-15936-img0001.jpg "Western Blot: NEK7 Antibody (7Y3B10) [NBP3-15936] - Western blot analysis of extracts of various cell lines, using NEK7 Rabbit mAb (NBP3-15936) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: NEK7 Antibody (S04-7A1) [NBP3-19713] - Western blot detection of NEK7 in Rat Brain, C6 cell lysates using NBP3-19713 (1:1000 diluted). Predicted band size: 35kDa. Observed band size: 35kDa.](http://images.novusbio.com/fullsize/NEK7-Antibody-S04-7A1-Western-Blot-NBP3-19713-img0001.jpg "Western Blot: NEK7 Antibody (S04-7A1) [NBP3-19713] - Western blot detection of NEK7 in Rat Brain, C6 cell lysates using NBP3-19713 (1:1000 diluted). Predicted band size: 35kDa. Observed band size: 35kDa.")
![Immunohistochemistry: NEK7 Antibody [NBP1-86457] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/NEK7-Antibody-Immunohistochemistry-NBP1-86457-img0003.jpg "Immunohistochemistry: NEK7 Antibody [NBP1-86457] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.")
![Western Blot: NEK7 Overexpression Lysate (Adult Normal) [NBL1-13587] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NEK7.](http://images.novusbio.com/fullsize/NEK7-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13587-img0002.jpg "Western Blot: NEK7 Overexpression Lysate (Adult Normal) [NBL1-13587] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NEK7.")
![SDS-Page: Recombinant Human NEK7 Protein [NBP2-23287]](http://images.novusbio.com/fullsize/NEK7-Protein-SDS-Page-NBP2-23287-img0001.jpg "SDS-Page: Recombinant Human NEK7 Protein [NBP2-23287]")