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![Western Blot: EB3 Antibody [NB100-56441] - Analysis on 15 ug of human brain lysate.](http://images.novusbio.com/fullsize/EB3-Antibody-Western-Blot-NB100-56441-img0002.jpg "Western Blot: EB3 Antibody [NB100-56441] - Analysis on 15 ug of human brain lysate.")
![Western Blot: EB3 Antibody (7J9V3) [NBP3-15904] - Western blot analysis of extracts of various cell lines, using EB3 Rabbit mAb (NBP3-15904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/EB3-Antibody-7J9V3-Western-Blot-NBP3-15904-img0004.jpg "Western Blot: EB3 Antibody (7J9V3) [NBP3-15904] - Western blot analysis of extracts of various cell lines, using EB3 Rabbit mAb (NBP3-15904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of U-2 OS cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112146..jpg)
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of C6 cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112350..jpg)
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (7J9V3) [NBP3-15904] -Confocal imaging of C2C12 cells using EB3/MAPRE3 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15904_rabbit-eb3-mab-7j9v3-immunocytochemistry-immunofluorescence-1652024112518..jpg)
![Western Blot: EB3 Antibody (KT36) [NBP3-23294] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.](http://images.novusbio.com/fullsize/nbp3-23294_rat-eb3-mab-kt36-11920231633169.jpg "Western Blot: EB3 Antibody (KT36) [NBP3-23294] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.")
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (KT36) [NBP3-23294] - Immunocytochemical labeling of EB3 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (green) and rat monoclonal anti-EB3 (NBP3-23294) (red). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat:DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23294_rat-eb3-mab-kt36-19920231856937.jpg)
![Western Blot: EB3 Antibody [NBP1-84924] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/EB3-Antibody-Western-Blot-NBP1-84924-img0006.jpg "Western Blot: EB3 Antibody [NBP1-84924] - Analysis in human cell line U-87 MG.")
![Immunohistochemistry-Paraffin: EB3 Antibody [NBP1-84924] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/EB3-Antibody-Immunohistochemistry-Paraffin-NBP1-84924-img0001.jpg)
![Western Blot: EB3 Antibody (KT53) [NBP3-23293] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.](http://images.novusbio.com/fullsize/nbp3-23293_rat-eb3-mab-kt53-199202312523647.jpg "Western Blot: EB3 Antibody (KT53) [NBP3-23293] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.")
![Western Blot: EB3 [p Ser176] Antibody [NBP3-23300] - Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (NBP3-23294) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).](http://images.novusbio.com/fullsize/nbp3-23300_rabbit-eb3-p-ser176-pab-19920231992810.jpg "Western Blot: EB3 [p Ser176] Antibody [NBP3-23300] - Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (NBP3-23294) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).")
![Western Blot: EB3 [p Ser162] Antibody [NBP3-23299] - Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (NBP3-23294) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).](http://images.novusbio.com/fullsize/nbp3-23299_rabbit-eb3-p-ser162-pab-19920231915419.jpg "Western Blot: EB3 [p Ser162] Antibody [NBP3-23299] - Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (NBP3-23294) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).")
![Western Blot: EB3 Antibody [NBP3-23298] - Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (NBP3-23294) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).](http://images.novusbio.com/fullsize/nbp3-23298_rabbit-eb3-pab-19920231856922.jpg "Western Blot: EB3 Antibody [NBP3-23298] - Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (NBP3-23294) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).")
![Western Blot: EB3 Antibody [NBP2-87319] - Host: Rabbit. Target Name: MAPRE3. Sample Tissue: Human Stomach Tumor. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/EB3-Antibody-Western-Blot-NBP2-87319-img0001.jpg "Western Blot: EB3 Antibody [NBP2-87319] - Host: Rabbit. Target Name: MAPRE3. Sample Tissue: Human Stomach Tumor. Antibody Dilution: 1.0ug/ml")
![Western Blot: EB3 Overexpression Lysate (Adult Normal) [NBL1-12893] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EB3.](http://images.novusbio.com/fullsize/EB3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12893-img0002.jpg "Western Blot: EB3 Overexpression Lysate (Adult Normal) [NBL1-12893] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EB3.")
![Western Blot: EB3 Antibody [NBP2-92839] - Analysis of extracts of various cell lines, using EB3 .Exposure time: 90s.](http://images.novusbio.com/fullsize/EB3-Antibody-Western-Blot-NBP2-92839-img0002.jpg "Western Blot: EB3 Antibody [NBP2-92839] - Analysis of extracts of various cell lines, using EB3 .Exposure time: 90s.")