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![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Leukotriene A4 hydrolasemonoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Western-Blot-NBP1-47829-img0022.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Leukotriene A4 hydrolasemonoclonal antibody.")
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded prostate tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0020.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Leukotriene A4 hydrolase(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Leukotriene A4 hydrolase.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Western-Blot-NBP1-47829-img0021.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0013.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0014.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded endometrium tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0018.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded Ovary tissue using anti-Leukotriene A4 hydrolase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0017.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Staining of paraffin-embedded pancreas tissue using anti-Leukotriene A4 hydrolasemouse monoclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunohistochemistry-Paraffin-NBP1-47829-img0019.jpg)
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody (OTI1E9) [NBP1-47829] - Immunoprecipitation of LTA4H by using TrueMab monoclonal anti-LTA4H antibody (Negative control: IP without adding anti-LTA4H antibody). For each experiment, 500ul of DDK tagged LTA4H overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant-LTA4H antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-OTI1E9-Immunoprecipitation-NBP1-47829-img0023.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90368] - Analysis using Anti-LTA4H antibody NBP1-90368 (A) shows similar pattern to independent antibody NBP1-90375 (B).](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Western-Blot-NBP1-90368-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90368] - Analysis using Anti-LTA4H antibody NBP1-90368 (A) shows similar pattern to independent antibody NBP1-90375 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90368] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90368-img0006.jpg)
![Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90368] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Immunohistochemistry-Paraffin-NBP1-90368-img0002.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-201-Western-Blot-NBP2-89374-img0002.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa")
![Immunocytochemistry/Immunofluorescence: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Staining of Human LTA4H in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LTA4H monoclonal antibody (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-201-Immunocytochemistry-Immunofluorescence-NBP2-89374-img0001.jpg)
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-LTA4H rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LTA4H rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 110 kDa Observed band size: 110 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-201-Immunoprecipitation-NBP2-89374-img0003.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (SR2135) [NBP3-22100] - Western blot analysis using NBP3-22100 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22100_rabbit-leukotriene-a4-hydrolase-lta4h-mab-sr2135-5720231784610.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (SR2135) [NBP3-22100] - Western blot analysis using NBP3-22100 in A549 cell lysate.")
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90375] - Analysis using Anti-LTA4H antibody NBP1-90375 (A) shows similar pattern to independent antibody NBP1-90368 (B).](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Western-Blot-NBP1-90375-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90375] - Analysis using Anti-LTA4H antibody NBP1-90375 (A) shows similar pattern to independent antibody NBP1-90368 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP1-90375] - Staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Immunohistochemistry-Paraffin-NBP1-90375-img0001.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Western-Blot-NBP2-99029-img0003.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).")
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Western-Blot-NBP2-99029-img0002.jpg)
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Leukotriene A4 Hydrolase/LTA4H was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 2 ul anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Immunoprecipitation-NBP2-99029-img0001.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (6G7) [NBP3-26161] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, 293T whole cell lysate, PC-3 whole cell lysate, Mouse Brain whole cell lysate. All lanes: Leukotriene A4 Hydrolase/LTA4H Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70, 60, 58, 67 kDa Observed band size: 70 kDa](http://images.novusbio.com/fullsize/nbp3-26161_rabbit-leukotriene-a4-hydrolase-lta4h-mab-6g7-282202411442629.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (6G7) [NBP3-26161] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, 293T whole cell lysate, PC-3 whole cell lysate, Mouse Brain whole cell lysate. All lanes: Leukotriene A4 Hydrolase/LTA4H Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 70, 60, 58, 67 kDa Observed band size: 70 kDa")
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-19347] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Western-Blot-NBP2-19347-img0002.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-19347] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Leukotriene A4 hydrolase Antibody [NBP2-19348] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Leukotriene-A4-hydrolase-Antibody-Western-Blot-NBP2-19348-img0001.jpg "Western Blot: Leukotriene A4 hydrolase Antibody [NBP2-19348] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Leukotriene A4 hydrolase Overexpression Lysate (Adult Normal) [NBL1-12736] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Leukotriene A4 hydrolase.](http://images.novusbio.com/fullsize/Leukotriene-A4-hydrolase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12736-img0002.jpg "Western Blot: Leukotriene A4 hydrolase Overexpression Lysate (Adult Normal) [NBL1-12736] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Leukotriene A4 hydrolase.")